Enhanced detergent extraction for analysis of membrane proteomes by two-dimensional gel electrophoresis

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Enhanced detergent extraction for analysis of membrane proteomes by two-dimensional gel electrophoresis is …
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scholarly articleQ13442814

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P6179Dimensions Publication ID1006906166
P356DOI10.1186/1477-5956-3-5
P932PMC publication ID1184097
P698PubMed publication ID15941475
P5875ResearchGate publication ID7801871

P2093author name stringJens R Coorssen
Matthew A Churchward
John C Lang
R Hussain Butt
Kimberly K Hsu
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Postfractionation for enhanced proteomic analyses: routine electrophoretic methods increase the resolution of standard 2D-PAGE.Q48857564
Solubility of hydrophobic surfactant proteins in organic solvent/water mixtures. Structural studies on SP-B and SP-C in aqueous organic solvents and lipidsQ61642691
Dodecyl maltoside detergent improves resolution of hepatic membrane proteins in two-dimensional gelsQ67781964
Fractionation of human red cell membrane proteins by ion-exchange chromatography in detergent on Mono Q, with special reference to the glucose transporterQ70816312
Active unit of solubilized sarcoplasmic reticulum calcium adenosine triphosphatase: an active enzyme centrifugation analysisQ72787253
Modification of the Laemmli sodium dodecyl sulfate-polyacrylamide gel electrophoresis procedure to eliminate artifacts on reducing and nonreducing gelsQ73123147
Membrane proteomics: use of additive main effects with multiplicative interaction model to classify plasma membrane proteins according to their solubility and electrophoretic propertiesQ73192888
A method for global analysis of complex proteomes using sample prefractionation by solution isoelectrofocusing prior to two-dimensional electrophoresisQ74240323
Quantitative femto- to attomole immunodetection of regulated secretory vesicle proteins critical to exocytosisQ74511132
New zwitterionic detergents improve the analysis of membrane proteins by two-dimensional electrophoresisQ77308575
Protein delipidation and precipitation by tri-n-butylphosphate, acetone, and methanol treatment for isoelectric focusing and two-dimensional gel electrophoresisQ78198121
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequencesQ24336098
Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organismsQ24673118
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Q25938983
Subproteomics: identification of plasma membrane proteins from the yeast Saccharomyces cerevisiaeQ27937960
Comparative sequencing of human and chimpanzee MHC class I regions unveils insertions/deletions as the major path to genomic divergenceQ28178300
Molecular diversity of L-type calcium channels. Evidence for alternative splicing of the transcripts of three non-allelic genesQ28242683
Cloning and characterization of band 3, the human erythrocyte anion-exchange protein (AE1)Q28609272
The proteolipid of the A(1)A(0) ATP synthase from Methanococcus jannaschii has six predicted transmembrane helices but only two proton-translocating carboxyl groupsQ30760718
Reduction and alkylation of proteins in preparation of two-dimensional map analysis: why, when, and how?Q30996212
Membrane proteins and proteomics: un amour impossible?Q33901655
Evidence that the spectrin network and a nonosmotic force control the fusion product morphology in electrofused erythrocyte ghostsQ34088073
Overexpression, solubilization and purification of rat and human olfactory receptorsQ34384996
Biochemical and functional studies of cortical vesicle fusion: the SNARE complex and Ca2+ sensitivityQ36328525
Preparative electrophoretic method for the purification of a hydrophobic membrane protein: subunit c of the mitochondrial ATP synthase from rat liverQ40799181
Efficient encapsulation of antisense oligonucleotides in lipid vesicles using ionizable aminolipids: formation of novel small multilamellar vesicle structuresQ41630941
Reconstitution of the L-lactate carrier from rat and rabbit erythrocyte plasma membranesQ42046166
Solubilization of yeast plasma membranes and mitochondria by different types of non-denaturing detergentsQ42247199
Improved sensitivity proteomics by postharvest alkylation and radioactive labelling of proteinsQ43506225
Chaperone-like properties of lysophospholipidsQ43821106
Structure-efficiency relationships of zwitterionic detergents as protein solubilizers in two-dimensional electrophoresis.Q44325354
Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresisQ44355914
Solubilization of trichloroacetic acid (TCA) precipitated microbial proteins via naOH for two-dimensional electrophoresisQ44366139
Amidosulfobetaines, a family of detergents with improved solubilization properties: application for isoelectric focusing under denaturing conditionsQ44567327
Membrane proteome analysis of the green-sulfur bacterium Chlorobium tepidumQ45111619
Analysis of membrane proteins by two-dimensional electrophoresis: comparison of the proteins extracted from normal or Plasmodium falciparum-infected erythrocyte ghostsQ47857188
Improved protein solubility in two-dimensional electrophoresis using tributyl phosphine as reducing agent.Q47965088
P4510describes a project that usesImageQuantQ112270642
P433issue1
P407language of work or nameEnglishQ1860
P921main subjectelectrophoresisQ185098
P304page(s)5
P577publication date2005-06-07
P1433published inProteome ScienceQ15754354
P1476titleEnhanced detergent extraction for analysis of membrane proteomes by two-dimensional gel electrophoresis
P478volume3

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cites work (P2860)
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