The neutron structure of urate oxidase resolves a long-standing mechanistic conundrum and reveals unexpected changes in protonation

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The neutron structure of urate oxidase resolves a long-standing mechanistic conundrum and reveals unexpected changes in protonation is …
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scholarly articleQ13442814

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P819ADS bibcode2014PLoSO...986651O
P356DOI10.1371/JOURNAL.PONE.0086651
P3181OpenCitations bibliographic resource ID5000197
P932PMC publication ID3900588
P698PubMed publication ID24466188
P5875ResearchGate publication ID259920633

P50authorUlf RydeQ71347778
Esko OksanenQ79289384
Matthew P BlakeleyQ40364357
P2093author name stringMonika Budayova-Spano
Mohamed El-Hajji
P2860cites workA methodology and an instrument for the temperature-controlled optimization of crystal growth.Q51059371
Kinetic mechanism and cofactor content of soybean root nodule urate oxidase.Q54567537
Spectroscopic characterization of intermediates in the urate oxidase reaction.Q55067778
Free radical metabolite of uric acidQ69816664
The coordination of the catalytic zinc in alcohol dehydrogenase studied by combined quantum-chemical and molecular mechanics calculationsQ71347782
PHENIX: a comprehensive Python-based system for macromolecular structure solutionQ24654617
Near-atomic resolution structures of urate oxidase complexed with its substrate and analogues: the protonation state of the ligandQ27662093
X-ray, ESR, and quantum mechanics studies unravel a spin well in the cofactor-less urate oxidaseQ27667218
Coot: model-building tools for molecular graphicsQ27860505
Refinement of macromolecular structures by the maximum-likelihood methodQ27861011
PHENIX: building new software for automated crystallographic structure determinationQ27861044
MOLREP: an Automated Program for Molecular ReplacementQ29642797
Large crystal growth by thermal control allows combined X-ray and neutron crystallographic studies to elucidate the protonation states in Aspergillus flavus urate oxidase.Q33747171
Identification and purification of hydroxyisourate hydrolase, a novel ureide-metabolizing enzymeQ33880800
Crystal structure of the protein drug urate oxidase-inhibitor complex at 2.05 A resolution.Q36889898
Protein crystallography under xenon and nitrous oxide pressure: comparison with in vivo pharmacology studies and implications for the mechanism of inhaled anesthetic action.Q41048067
A preliminary neutron diffraction study of rasburicase, a recombinant urate oxidase enzyme, complexed with 8-azaxanthinQ42144350
The mechanism of the action of uricaseQ42951953
Intrinsic reactivity of uric acid with dioxygen: Towards the elucidation of the catalytic mechanism of urate oxidase.Q43874619
General base catalysis in the urate oxidase reaction: evidence for a novel Thr-Lys catalytic diadQ44393761
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue1
P407language of work or nameEnglishQ1860
P304page(s)e86651
P577publication date2014-01-01
P1433published inPLOS OneQ564954
P1476titleThe Neutron Structure of Urate Oxidase Resolves a Long-Standing Mechanistic Conundrum and Reveals Unexpected Changes in Protonation
P478volume9

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cites work (P2860)
Q37591354An extended N-H bond, driven by a conserved second-order interaction, orients the flavin N5 orbital in cholesterol oxidase
Q38851427Back-exchange of deuterium in neutron crystallography: characterization by IR spectroscopy.
Q47692181Catalytic Mechanisms for Cofactor-Free Oxidase-Catalyzed Reactions: Reaction Pathways of Uricase-Catalyzed Oxidation and Hydration of Uric Acid.
Q38972429Combining X-ray and neutron crystallography with spectroscopy
Q27698539Crystal structure of Bacillus fastidious uricase reveals an unexpected folding of the C-terminus residues crucial for thermostability under physiological conditions
Q30400856Neutron Crystallography for the Study of Hydrogen Bonds in Macromolecules.
Q26743427New insight into cofactor-free oxygenation from combined experimental and computational approaches
Q53107458On the Permeation by Dioxygen of Urate Oxidase from Aspergillus flavus in Complex with Xanthine Anion: Dioxygen Pathways and a Portrait of the Enzyme Cavities from Molecular Dynamics Simulations in Water Solution.
Q27696320Perdeuteration: improved visualization of solvent structure in neutron macromolecular crystallography
Q40364045Production, crystallization and neutron diffraction of fully deuterated human myelin peripheral membrane protein P2.
Q36931131Structure of a prereaction complex between the nerve agent sarin, its biological target acetylcholinesterase, and the antidote HI-6.
Q26801556Sub-atomic resolution X-ray crystallography and neutron crystallography: promise, challenges and potential
Q36517043Uricase alkaline enzymosomes with enhanced stabilities and anti-hyperuricemia effects induced by favorable microenvironmental changes
Q27316736XModeScore: a novel method for accurate protonation/tautomer-state determination using quantum-mechanically driven macromolecular X-ray crystallographic refinement

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