Screening for hydrolytic enzymes reveals Ayr1p as a novel triacylglycerol lipase in Saccharomyces cerevisiae

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Screening for hydrolytic enzymes reveals Ayr1p as a novel triacylglycerol lipase in Saccharomyces cerevisiae is …
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scholarly articleQ13442814

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P356DOI10.1074/JBC.M113.509927
P8608Fatcat IDrelease_4xhumdvlxrc5lkt2igacuu6l5a
P3181OpenCitations bibliographic resource ID1352893
P932PMC publication ID3861654
P698PubMed publication ID24187129

P50authorGünther DaumQ30171853
Albin HermetterQ30171862
Birgit PloierQ30171981
P2093author name stringClaudia Schmidt
Gerald Rechberger
Manfred Kollroser
Barbara Koch
Jessica Schatte
Melanie Scharwey
P2860cites workProtein measurement with the Folin phenol reagentQ20900776
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Phospholipid synthesis and lipid composition of subcellular membranes in the unicellular eukaryote Saccharomyces cerevisiaeQ24678561
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Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodQ25938999
A simple method for the isolation and purification of total lipides from animal tissuesQ25939009
Lipases and alpha/beta hydrolase foldQ34447424
A fatty acyl-CoA oxidizing system in rat liver peroxisomes; enhancement by clofibrate, a hypolipidemic drugQ34585642
An intimate collaboration between peroxisomes and lipid bodiesQ36117598
Activity-based proteomics: enzymatic activity profiling in complex proteomes.Q36506336
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Comparative enzymology of beta-oxidationQ39534414
Isolation and biochemical characterization of organelles from the yeast, Saccharomyces cerevisiaeQ40465413
Regulation of the yeast triacylglycerol lipase TGl3p by formation of nonpolar lipids.Q41979758
Oleate inhibits steryl ester synthesis and causes liposensitivity in yeast.Q42079508
Phosphatidylethanolamine synthesized by three different pathways is supplied to peroxisomes of the yeast Saccharomyces cerevisiaeQ43260978
Dehydroepiandrosterone (DHEA) metabolism in Saccharomyces cerevisiae expressing mammalian steroid hydroxylase CYP7B: Ayr1p and Fox2p display 17beta-hydroxysteroid dehydrogenase activityQ44060206
Fluorescent organophosphonates as inhibitors of microbial lipasesQ44589997
The lipid droplet enzyme Tgl1p hydrolyzes both steryl esters and triglycerides in the yeast, Saccharomyces cerevisiaeQ46515916
The lipolytic proteome of mouse adipose tissueQ46622399
Novel fluorescent phosphonic acid esters for discrimination of lipases and esterasesQ46645330
Inhibitor and protein microarrays for activity-based recognition of lipolytic enzymesQ46937969
13 Immunochemical identification of membrane proteins after sodium dodecyl sulfate—polyacrylamide gel electrophoresisQ72802839
Structural and biochemical properties of lipid particles from the yeast Saccharomyces cerevisiaeQ81136509
Analysis of lipid particles from yeastQ84570869
Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiaeQ27861085
The Saccharomyces cerevisiae EHT1 and EEB1 genes encode novel enzymes with medium-chain fatty acid ethyl ester synthesis and hydrolysis capacity.Q27931159
Janus-faced enzymes yeast Tgl3p and Tgl5p catalyze lipase and acyltransferase reactionsQ27931469
Obese yeast: triglyceride lipolysis is functionally conserved from mammals to yeastQ27931759
YEH2/YLR020c encodes a novel steryl ester hydrolase of the yeast Saccharomyces cerevisiaeQ27932534
YMR313c/TGL3 encodes a novel triacylglycerol lipase located in lipid particles of Saccharomyces cerevisiaeQ27932920
Synthesis of triacylglycerols by the acyl-coenzyme A:diacyl-glycerol acyltransferase Dga1p in lipid particles of the yeast Saccharomyces cerevisiaeQ27933343
The biochemistry of peroxisomal beta-oxidation in the yeast Saccharomyces cerevisiaeQ27933605
The Saccharomyces cerevisiae YLL012/YEH1, YLR020/YEH2, and TGL1 genes encode a novel family of membrane-anchored lipases that are required for steryl ester hydrolysisQ27933795
A lecithin cholesterol acyltransferase-like gene mediates diacylglycerol esterification in yeast.Q27933801
The putative Saccharomyces cerevisiae hydrolase Ldh1p is localized to lipid dropletsQ27933948
Tgl4p and Tgl5p, two triacylglycerol lipases of the yeast Saccharomyces cerevisiae are localized to lipid particlesQ27934410
Lpx1p is a peroxisomal lipase required for normal peroxisome morphology.Q27935104
Multiple functions as lipase, steryl ester hydrolase, phospholipase, and acyltransferase of Tgl4p from the yeast Saccharomyces cerevisiaeQ27935197
Lipid particles/droplets of the yeast Saccharomyces cerevisiae revisited: lipidome meets proteomeQ27936020
Identification and characterization of major lipid particle proteins of the yeast Saccharomyces cerevisiaeQ27936107
Identification of Yju3p as functional orthologue of mammalian monoglyceride lipase in the yeast SaccharomycescerevisiaeQ27936792
Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteinsQ27937249
Contribution of Are1p and Are2p to steryl ester synthesis in the yeast Saccharomyces cerevisiaeQ27937712
Involvement of the Saccharomyces cerevisiae hydrolase Ldh1p in lipid homeostasisQ27937766
1-Acyldihydroxyacetone-phosphate reductase (Ayr1p) of the yeast Saccharomyces cerevisiae encoded by the open reading frame YIL124w is a major component of lipid particlesQ27939578
The Saccharomyces cerevisiae TSC10/YBR265w gene encoding 3-ketosphinganine reductase is identified in a screen for temperature-sensitive suppressors of the Ca2+-sensitive csg2Delta mutantQ27940195
Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedureQ28131658
Sterol esterification in yeast: a two-gene processQ28280662
Molecular characterization of the patatin multigene family of potatoQ28297213
Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiaeQ29546523
Lipid droplets and peroxisomes: key players in cellular lipid homeostasis or a matter of fat--store 'em up or burn 'em downQ30425235
The DGA1 gene determines a second triglyceride synthetic pathway in yeast.Q30667251
P433issue50
P407language of work or nameEnglishQ1860
P921main subjectcell biologyQ7141
Saccharomyces cerevisiaeQ719725
Triglyceride lipase YOR084WQ27548611
Acylglycerone-phosphate reductase YIL124WQ27551370
P304page(s)36061-72
P577publication date2013-12-13
P1433published inJournal of Biological ChemistryQ867727
P1476titleScreening for hydrolytic enzymes reveals Ayr1p as a novel triacylglycerol lipase in Saccharomyces cerevisiae
P478volume288

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