The purified yeast pre-mRNA splicing factor PRP2 is an RNA-dependent NTPase.

scientific article

The purified yeast pre-mRNA splicing factor PRP2 is an RNA-dependent NTPase. is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1002/J.1460-2075.1992.TB05291.X
P932PMC publication ID556699
P698PubMed publication ID1534753

P2093author name stringS H Kim
J Smith
R J Lin
A Claude
P2860cites workA cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene familyQ27929779
PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosomeQ27937219
Interactions of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiaeQ27938316
High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrierQ28131646
Silver staining of proteins in polyacrylamide gelsQ29616239
Messenger RNA Splicing in Yeast: Clues to Why the Spliceosome Is a RibonucleoproteinQ29618253
Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiaeQ29620268
Bidirectional RNA helicase activity of eucaryotic translation initiation factors 4A and 4FQ29620295
Birth of the D-E-A-D boxQ29620352
Yeast mRNA splicing in vitroQ29620824
PRP5: a helicase-like protein required for mRNA splicing in yeastQ33612385
A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicingQ33838525
RNA Processing and the Intervening Sequence ProblemQ34210540
Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteinsQ36216616
PRP18, a protein required for the second reaction in pre-mRNA splicingQ36714216
Biochemical Mechanisms of Constitutive and Regulated Pre-mRNA SplicingQ37132263
Pre-mRNA splicing in yeastQ37716836
The role of small nuclear ribonucleoprotein particles in pre-mRNA splicingQ39481136
The yeast PRP2 protein, a putative RNA-dependent ATPase, shares extensive sequence homology with two other pre-mRNA splicing factorsQ40525857
RNA helicase: a novel activity associated with a protein encoded by a positive strand RNA virusQ40526962
Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomesQ44165085
An eIF-4A-like protein is a suppressor of an Escherichia coli mutant defective in 50S ribosomal subunit assemblyQ44628929
Splicing of yeast nuclear pre-mRNA in vitro requires a functional 40S spliceosome and several extrinsic factorsQ46593105
The isolation and characterization of an RNA helicase from nuclear extracts of HeLa cells.Q50799251
Mechanisms of Alternative Pre-mRNA SplicingQ52446396
RNA helicase activity associated with the human p68 proteinQ58376470
Alive with DEAD proteinsQ59076947
The yeast RNA gene products are essential for mRNA splicing in vitroQ59198319
Identification of the RNA2 protein of Saccharomyces cerevisiaeQ68247706
Splicing of messenger RNA precursorsQ68931516
14 Purification of recA protein from Escherichia coliQ70284322
Identification of ten genes that control ribosome formation in yeastQ71613205
P433issue6
P407language of work or nameEnglishQ1860
P921main subjectDEAH-box RNA-dependent ATPase PRP2 YNR011CQ27552968
P304page(s)2319-2326
P577publication date1992-06-01
P1433published inThe EMBO JournalQ1278554
P1476titleThe purified yeast pre-mRNA splicing factor PRP2 is an RNA-dependent NTPase
P478volume11

Reverse relations

cites work (P2860)
Q33938648A conformational rearrangement in the spliceosome is dependent on PRP16 and ATP hydrolysis
Q34788134A conformational rearrangement in the spliceosome sets the stage for Prp22-dependent mRNA release
Q27939993A link between secretion and pre-mRNA processing defects in Saccharomyces cerevisiae and the identification of a novel splicing gene, RSE1
Q34608096A mutation in a methionine tRNA gene suppresses the prp2-1 Ts mutation and causes a pre-mRNA splicing defect in Saccharomyces cerevisiae
Q35967994A temperature-sensitive allele of a putative mRNA splicing helicase down-regulates many cell wall genes and causes radial swelling in Arabidopsis thaliana.
Q47636352Analysis of small nuclear RNAs in a precatalytic spliceosome.
Q30559916Biased Brownian ratcheting leads to pre-mRNA remodeling and capture prior to first-step splicing
Q24644322Bicaudal D induces selective dynein-mediated microtubule minus end-directed transport
Q45755686Biochemical and immunological characterization of rice homologues of the human immunodeficiency virus-1 Tat binding protein and subunit 4 of human 26S proteasome subunits
Q29619863DEAD-box proteins: the driving forces behind RNA metabolism
Q27934203Dbp5p, a cytosolic RNA helicase, is required for poly(A)+ RNA export
Q27937592Dbp6p is an essential putative ATP-dependent RNA helicase required for 60S-ribosomal-subunit assembly in Saccharomyces cerevisiae
Q27933064Dbp7p, a putative ATP-dependent RNA helicase from Saccharomyces cerevisiae, is required for 60S ribosomal subunit assembly
Q24535957Ddx42p--a human DEAD box protein with RNA chaperone activities
Q34365562Definition of a spliceosome interaction domain in yeast Prp2 ATPase
Q40400441Detection of dsRNA-binding domains in RNA helicase A and Drosophila maleless: implications for monomeric RNA helicases
Q34362876Dominant negative mutants of the yeast splicing factor Prp2 map to a putative cleft region in the helicase domain of DExD/H-box proteins.
Q40805562Effects of secondary structure on pre-mRNA splicing: hairpins sequestering the 5' but not the 3' splice site inhibit intron processing in Nicotiana plumbaginifolia.
Q37059609Exon ligation is proofread by the DExD/H-box ATPase Prp22p
Q27931519Fal1p is an essential DEAD-box protein involved in 40S-ribosomal-subunit biogenesis in Saccharomyces cerevisiae
Q34825409Formation of the yeast splicing complex A1 and association of the splicing factor PRP19 with the pre-mRNA are independent of the 3' region of the intron
Q88532204Functional and Biochemical Characterization of Dib1's Role in Pre-Messenger RNA Splicing
Q27939025Functional association of essential splicing factor(s) with PRP19 in a protein complex
Q34706824GPKOW is essential for pre-mRNA splicing in vitro and suppresses splicing defect caused by dominant-negative DHX16 mutation in vivo
Q33963175Genetic interactions between the yeast RNA helicase homolog Prp16 and spliceosomal snRNAs identify candidate ligands for the Prp16 RNA-dependent ATPase
Q38358262Genetic studies of the PRP17 gene of Saccharomyces cerevisiae: a domain essential for function maps to a nonconserved region of the protein
Q56657141Helicases: amino acid sequence comparisons and structure-function relationships
Q48069855Homeless is required for RNA localization in Drosophila oogenesis and encodes a new member of the DE-H family of RNA-dependent ATPases
Q24318692Human helicase gene SKI2W in the HLA class III region exhibits striking structural similarities to the yeast antiviral gene SKI2 and to the human gene KIAA0052: emergence of a new gene family
Q24338181Identification of DHX33 as a mediator of rRNA synthesis and cell growth
Q24324207Identification of a putative RNA helicase (HRH1), a human homolog of yeast Prp22
Q24546120Identification of an RNA-dependent ATPase activity in mammalian U5 snRNPs
Q40419046Interaction between a G-patch protein and a spliceosomal DEXD/H-box ATPase that is critical for splicing
Q27938081Mechanical devices of the spliceosome: motors, clocks, springs, and things
Q47738832Mechanistic insights into precursor messenger RNA splicing by the spliceosome
Q27932768Mutational analysis of the PRP4 protein of Saccharomyces cerevisiae suggests domain structure and snRNP interactions
Q27485864Mutational analysis of the hepatitis C virus RNA helicase
Q34604696Mutational analysis of the yeast DEAH-box splicing factor Prp16.
Q38293067Mutational analysis of vaccinia virus nucleoside triphosphate phosphohydrolase II, a DExH box RNA helicase
Q42397657Mutations in PRP43 that uncouple RNA-dependent NTPase activity and pre-mRNA splicing function
Q27936960Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome
Q40395216Overexpression of DEAD box protein pMSS116 promotes ATP-dependent splicing of a yeast group II intronin vitro
Q24539259PRP16, a DEAH-box RNA helicase, is recruited to the spliceosome primarily via its nonconserved N-terminal domain
Q36676876PRP19: a novel spliceosomal component
Q72063308Pre-mRNA splicing
Q27937849Pre-mRNA splicing within an assembled yeast spliceosome requires an RNA-dependent ATPase and ATP hydrolysis
Q48081875Prevalence and distribution of introns in non-ribosomal protein genes of yeast
Q27935431Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS.
Q27934705Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing
Q24601157Purification of splicing factor SF1, a heat-stable protein that functions in the assembly of a presplicing complex
Q37323177RAD25 (SSL2), the yeast homolog of the human xeroderma pigmentosum group B DNA repair gene, is essential for viability
Q40388300RNA helicase dynamics in pre-mRNA splicing
Q35236525RNA splicing in lower eukaryotes
Q27930438RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2
Q24336431RNA-unwinding and RNA-folding activities of RNA helicase II/Gu--two activities in separate domains of the same protein
Q41533943Roles of PRP8 protein in the assembly of splicing complexes
Q40789125SLU7 and a novel activity, SSF1, act during the PRP16-dependent step of yeast pre-mRNA splicing
Q27939803Snt309p, a component of the Prp19p-associated complex that interacts with Prp19p and associates with the spliceosome simultaneously with or immediately after dissociation of U4 in the same manner as Prp19p
Q27930317Spb4p, an essential putative RNA helicase, is required for a late step in the assembly of 60S ribosomal subunits in Saccharomyces cerevisiae.
Q27931041Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing
Q27939472Splicing Factor Cwc22 Is Required for the Function of Prp2 and for the Spliceosome To Escape from a Futile Pathway
Q74453898Splicing factor Prp8 governs U4/U6 RNA unwinding during activation of the spliceosome
Q41288678Splicing of precursors to mRNA in higher plants: mechanism, regulation and sub-nuclear organisation of the spliceosomal machinery
Q92889690Structural analysis of the intrinsically disordered splicing factor Spp2 and its binding to the DEAH-box ATPase Prp2
Q40027412Structural and functional analysis of the RNA helicase Prp43 from the thermophilic eukaryote Chaetomium thermophilum
Q64069104Structural basis for RNA translocation by DEAH-box ATPases
Q27491211Structural basis for the function of DEAH helicases
Q39408998Studying structure and function of spliceosomal helicases
Q27938748The DEAH-box protein PRP22 is an ATPase that mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
Q46378287The DEAH-box splicing factor Prp16 unwinds RNA duplexes in vitro
Q27933083The DExD/H-box ATPase Prp2p destabilizes and proofreads the catalytic RNA core of the spliceosome
Q27938864The G-patch protein Spp2 couples the spliceosome-stimulated ATPase activity of the DEAH-box protein Prp2 to catalytic activation of the spliceosome
Q24324586The HeLa 200 kDa U5 snRNP-specific protein and its homologue in Saccharomyces cerevisiae are members of the DEXH-box protein family of putative RNA helicases
Q39873742The QRxGRxGRxxxG motif of the vaccinia virus DExH box RNA helicase NPH-II is required for ATP hydrolysis and RNA unwinding but not for RNA binding
Q50058804The RES complex is required for efficient transformation of the precatalytic B spliceosome into an activated Bact complex
Q34364667The conserved central domain of yeast U6 snRNA: importance of U2-U6 helix Ia in spliceosome assembly
Q27939776The final stages of spliceosome maturation require Spp2p that can interact with the DEAH box protein Prp2p and promote step 1 of splicing
Q40409686The fission yeast prp4+ gene involved in pre-mRNA splicing codes for a predicted serine/threonine kinase and is essential for growth
Q24323409The human U5-200kD DEXH-box protein unwinds U4/U6 RNA duplices in vitro
Q36435201The interaction of Prp2 with a defined region of the intron is required for the first splicing reaction
Q34466803The nucleoside triphosphatase and helicase activities of vaccinia virus NPH-II are essential for virus replication
Q27931001The splicing factor PRP2, a putative RNA helicase, interacts directly with pre-mRNA
Q36676911The yeast PRP19 protein is not tightly associated with small nuclear RNAs, but appears to associate with the spliceosome after binding of U2 to the pre-mRNA and prior to formation of the functional spliceosome
Q34932142The zebrafish maternal-effect gene mission impossible encodes the DEAH-box helicase Dhx16 and is essential for the expression of downstream endodermal genes
Q71942854Two-dimensional gel protein database of Saccharomyces cerevisiae
Q37302557Vaccinia virus RNA helicase: an essential enzyme related to the DE-H family of RNA-dependent NTPases
Q27937247Yeast precursor mRNA processing protein PRP19 associates with the spliceosome concomitant with or just after dissociation of U4 small nuclear RNA.
Q40776330Yeast protein splicing factors involved in nuclear pre-mRNA splicing

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