Nucleocytoplasmic distribution and dynamics of the autophagosome marker EGFP-LC3

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Nucleocytoplasmic distribution and dynamics of the autophagosome marker EGFP-LC3 is …
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scholarly articleQ13442814

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P819ADS bibcode2010PLoSO...5.9806D
P356DOI10.1371/JOURNAL.PONE.0009806
P3181OpenCitations bibliographic resource ID3162586
P932PMC publication ID2843706
P698PubMed publication ID20352102
P5875ResearchGate publication ID42639956

P2093author name stringAnne K Kenworthy
Kimberly R Drake
Minchul Kang
P2860cites workQuantitative FRAP in analysis of molecular binding dynamics in vivo.Q50652873
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Microtubule involvement in translational regulation of fibronectin expression by light chain 3 of microtubule-associated protein 1 in vascular smooth muscle cells.Q52531811
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The human homolog of Saccharomyces cerevisiae Apg7p is a Protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3Q24290562
HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugatesQ24296107
Phosphatidylserine in addition to phosphatidylethanolamine is an in vitro target of the mammalian Atg8 modifiers, LC3, GABARAP, and GATE-16Q24296712
The TP53INP2 protein is required for autophagy in mammalian cellsQ24321519
GATE-16 and GABARAP are authentic modifiers mediated by Apg7 and Apg3Q24338023
Mobility measurement by analysis of fluorescence photobleaching recovery kineticsQ24536888
LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processingQ24597817
The Atg16L complex specifies the site of LC3 lipidation for membrane biogenesis in autophagyQ24647053
Formation process of autophagosome is traced with Apg8/Aut7p in yeastQ24670615
The reversible modification regulates the membrane-binding state of Apg8/Aut7 essential for autophagy and the cytoplasm to vacuole targeting pathwayQ24678032
Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved regionQ24685870
Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusionQ27933991
Dynamic binding of histone H1 to chromatin in living cellsQ28141055
Beclin 1 contains a leucine-rich nuclear export signal that is required for its autophagy and tumor suppressor functionQ28213267
Molecular characterization of light chain 3. A microtubule binding subunit of MAP1A and MAP1BQ28237483
Solution structure of microtubule-associated protein light chain 3 and identification of its functional subdomainsQ28247288
LC3 conjugation system in mammalian autophagyQ28278334
Microtubules facilitate autophagosome formation and fusion of autophagosomes with endosomesQ28572782
Impairment of starvation-induced and constitutive autophagy in Atg7-deficient miceQ28588080
Methods in mammalian autophagy researchQ29547276
CRM1 is responsible for intracellular transport mediated by the nuclear export signalQ29547861
Autophagy as a regulated pathway of cellular degradationQ29547914
Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3Q29614176
Analysis and prediction of leucine-rich nuclear export signalsQ29619228
Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factorsQ29620194
An Atg4B mutant hampers the lipidation of LC3 paralogues and causes defects in autophagosome closureQ30484302
Intracellular macromolecular mobility measured by fluorescence recovery after photobleaching with confocal laser scanning microscopes.Q30500543
Studying protein dynamics in living cellsQ30674118
Analyzing intracellular binding and diffusion with continuous fluorescence photobleachingQ30921299
Fluorescence recovery after photobleaching (FRAP) methods for visualizing protein dynamics in living mammalian cell nucleiQ33197877
A generalization of theory for two-dimensional fluorescence recovery after photobleaching applicable to confocal laser scanning microscopesQ33498663
Crossing the nuclear envelope: hierarchical regulation of nucleocytoplasmic transportQ34008468
The crystal structure of microtubule-associated protein light chain 3, a mammalian homologue of Saccharomyces cerevisiae Atg8.Q34334691
Fluorescence recovery after photobleaching: application to nuclear proteinsQ34440301
The DNA binding activity of p53 displays reaction-diffusion kinetics.Q34680338
LC3 and AutophagyQ34772075
Methods for monitoring autophagyQ35869399
Nucleocytoplasmic shuttling revealed by FRAP and FLIP technologiesQ36049365
Depalmitoylated Ras traffics to and from the Golgi complex via a nonvesicular pathwayQ36320736
Evidence for a common mode of transcription factor interaction with chromatin as revealed by improved quantitative fluorescence recovery after photobleaching.Q36510561
Maturation of autophagic vacuoles in Mammalian cellsQ36549471
The itinerary of autophagosomes: from peripheral formation to kiss-and-run fusion with lysosomesQ36580007
The Nup358-RanGAP complex is required for efficient importin alpha/beta-dependent nuclear importQ36631203
Methods for monitoring autophagy from yeast to humanQ36708691
Microtubule-associated protein 1 light chain 3 is a fibronectin mRNA-binding protein linked to mRNA translation in lamb vascular smooth muscle cellsQ39795903
Non-autophagic GFP-LC3 puncta induced by saponin and other detergentsQ40085197
Cytosolic LC3 ratio as a sensitive index of macroautophagy in isolated rat hepatocytes and H4-II-E cellsQ40109908
LC3, an autophagosome marker, can be incorporated into protein aggregates independent of autophagy: caution in the interpretation of LC3 localizationQ40154056
Green fluorescent protein-tagging reduces the nucleocytoplasmic shuttling specifically of unphosphorylated STAT1.Q40173250
Real-time imaging of nuclear permeation by EGFP in single intact cellsQ40230214
The dynamics of autophagy visualized in live cells: from autophagosome formation to fusion with endo/lysosomesQ40250494
Nucleo-cytoplasmic distribution of beta-catenin is regulated by retention.Q40300945
Live Cell Imaging of ERK and MEK: simple binding equilibrium explains the regulated nucleocytoplasmic distribution of ERK.Q40491305
Light Chain 3 associates with a Sos1 guanine nucleotide exchange factor: its significance in the Sos1-mediated Rac1 signaling leading to membrane rufflingQ42809573
Utilizing flow cytometry to monitor autophagy in living mammalian cellsQ44821204
Measurement of dynamic protein binding to chromatin in vivo, using photobleaching microscopyQ47211494
Caldendrin but not calmodulin binds to light chain 3 of MAP1A/B: an association with the microtubule cytoskeleton highlighting exclusive binding partners for neuronal Ca(2+)-sensor proteins.Q47737220
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue3
P407language of work or nameEnglishQ1860
P921main subjectautophagosomeQ14882441
P304page(s)e9806
P577publication date2010-03-23
P1433published inPLOS OneQ564954
P1476titleNucleocytoplasmic distribution and dynamics of the autophagosome marker EGFP-LC3
P478volume5

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