Identification of an NADH-cytochrome b(5) reductase gene from an arachidonic acid-producing fungus, Mortierella alpina 1S-4, by sequencing of the encoding cDNA and heterologous expression in a fungus, Aspergillus oryzae

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Identification of an NADH-cytochrome b(5) reductase gene from an arachidonic acid-producing fungus, Mortierella alpina 1S-4, by sequencing of the encoding cDNA and heterologous expression in a fungus, Aspergillus oryzae is …
instance of (P31):
scholarly articleQ13442814

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P932PMC publication ID99714
P698PubMed publication ID10473389

P2093author name stringM Kobayashi
S Shimizu
E Sakuradani
P2860cites workRole of cytochrome b5 in hydroxylation by a reconstituted cytochrome P-450-containing systemQ68698548
Microsomal lipid peroxidation: mechanisms of initiation. The role of iron and iron chelatorsQ69174489
Complete amino acid sequence of steer liver microsomal NADH-cytochrome b5 reductaseQ69909160
Role of the electron transfer system in microsomal drug monooxygenase reaction catalyzed by cytochrome P-450Q70492083
Electron transfer by ferredoxin:NADP+ reductase. Rapid-reaction evidence for participation of a ternary complexQ70620888
Biochemical properties of cytochrome b5-dependent microsomal fatty acid elongation and identification of productsQ71401339
Primary Structure of Rabbit Liver Cytochrome b5*Q72186694
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Q25938983
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingQ25938984
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform ExtractionQ25938986
Phthalate dioxygenase reductase: a modular structure for electron transfer from pyridine nucleotides to [2Fe-2S]Q27641437
Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAsQ27860573
Studies on transformation of Escherichia coli with plasmidsQ27860598
Incomplete arrest in the outer membrane sorts NADH-cytochrome b5 reductase to two different submitochondrial compartmentsQ27936992
Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranesQ28131700
A function of cytochrome b5 in fatty acid desaturation by rat liver microsomesQ28253650
Arachidonic acid metabolismQ28287622
Mechanism of C-5 double bond introduction in the biosynthesis of cholesterol by rat liver microsomesQ28321184
Molecular cloning of a cDNA encoding rat NADH-cytochrome b5 reductase and the corresponding geneQ28574368
Specific arrangement of three amino acid residues for flavin-binding barrel structures in NADH-cytochrome b5 reductase and the other flavin-dependent reductasesQ30193585
Atomic structure of ferredoxin-NADP+ reductase: prototype for a structurally novel flavoenzyme familyQ30195728
Identification of Delta5-desaturase from Mortierella alpina by heterologous expression in Bakers' yeast and canolaQ31989196
Soluble cytochrome b 5 reductase from human erythrocytesQ34052175
Characterization of the interaction of amphipathic cytochrome b5 with stearyl coenzyme A desaturase and NADPH:cytochrome P-450 reductaseQ34245964
Crystal structure of the FAD-containing fragment of corn nitrate reductase at 2.5 A resolution: relationship to other flavoprotein reductasesQ34315273
The organization and the complete nucleotide sequence of the human NADH-cytochrome b5 reductase geneQ34418794
Cloning of the nitrate reductase gene (niaD) of Aspergillus nidulans and its use for transformation of Fusarium oxysporumQ35878926
Isolation and complete sequence of CBR, a gene encoding a putative cytochrome b reductase in Saccharomyces cerevisiaeQ36762502
A Novel Delta5-Desaturase-Defective Mutant of Mortierella alpina 1S-4 and Its Dihomo-gamma-Linolenic Acid ProductivityQ39894814
Screening of novel microbial enzymes for the production of biologically and chemically useful compoundsQ41431903
Rat liver microsomal elongation of fatty acids. Possible involvement of cytochrome b5Q41739551
Distribution of the integral membrane protein NADH-cytochrome b5 reductase in rat liver cells, studied with a quantitative radioimmunoblotting assayQ41886497
A single mRNA, transcribed from an alternative, erythroid-specific, promoter, codes for two non-myristylated forms of NADH-cytochrome b5 reductaseQ42597097
Disruption of the Saccharomyces cerevisiae gene for NADPH-cytochrome P450 reductase causes increased sensitivity to ketoconazoleQ45171125
Functional elements of the promoter region of the Aspergillus oryzae glaA gene encoding glucoamylaseQ46269680
Genetic analysis of cytochrome b5 from arachidonic acid-producing fungus, Mortierella alpina 1S-4: cloning, RNA editing and expression of the gene in Escherichia coli, and purification and characterization of the gene productQ47959860
Identification of Delta12-fatty acid desaturase from arachidonic acid-producing mortierella fungus by heterologous expression in the yeast Saccharomyces cerevisiae and the fungus Aspergillus oryzaeQ47970021
Isolation of a Delta5-fatty acid desaturase gene from Mortierella alpinaQ48036153
Deletion analysis of promoter elements of the Aspergillus oryzae agdA gene encoding alpha-glucosidaseQ48058359
Cloning and nucleotide sequence of the ribonuclease T1 gene (rntA) from Aspergillus oryzae and its expression in Saccharomyces cerevisiae and Aspergillus oryzaeQ48070152
Solubilization of NADH-cytochrome b5 reductase from liver microsomes by lysosomal digestion.Q53754066
Purification and properties of NADH-cytochrome b5 reductase solubilized by lysosomes from rat liver microsomes.Q53889646
P433issue9
P407language of work or nameEnglishQ1860
P921main subjectAspergillus oryzaeQ22662068
P304page(s)3873-3879
P577publication date1999-09-01
P1433published inApplied and Environmental MicrobiologyQ4781593
P1476titleIdentification of an NADH-cytochrome b(5) reductase gene from an arachidonic acid-producing fungus, Mortierella alpina 1S-4, by sequencing of the encoding cDNA and heterologous expression in a fungus, Aspergillus oryzae
P478volume65

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cites work (P2860)
Q36018952Analysis of cytochrome b(5) reductase-mediated metabolism in the phytopathogenic fungus Zymoseptoria tritici reveals novel functionalities implicated in virulence.
Q90405271Arachidonic acid production by the oleaginous fungus Mortierella alpina 1S-4: A review
Q35058223Cytochrome b₅ reductase-cytochrome b₅ as an active P450 redox enzyme system in Phanerochaete chrysosporium: atypical properties and in vivo evidence of electron transfer capability to CYP63A2
Q46434068Molecular evidence that the rate-limiting step for the biosynthesis of arachidonic acid in Mortierella alpina is at the level of an elongase

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