A refinement protocol to determine structure, topology, and depth of insertion of membrane proteins using hybrid solution and solid-state NMR restraints.

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A refinement protocol to determine structure, topology, and depth of insertion of membrane proteins using hybrid solution and solid-state NMR restraints. is …
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scholarly articleQ13442814

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P356DOI10.1007/S10858-009-9328-9
P932PMC publication ID2824793
P698PubMed publication ID19597943
P5875ResearchGate publication ID26666590

P50authorGianluigi VegliaQ91832414
P2093author name stringLei Shi
Alessandro Cembran
Jiali Gao
Nathaniel J Traaseth
Raffaello Verardi
P2860cites workNMR solution structure and topological orientation of monomeric phospholamban in dodecylphosphocholine micellesQ27642223
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A structural mechanism for MscS gating in lipid bilayersQ30435591
Combined NMR and EPR spectroscopy to determine structures of viral fusion domains in membranesQ30443827
Micelle-induced curvature in a water-insoluble HIV-1 Env peptide revealed by NMR dipolar coupling measurement in stretched polyacrylamide gel.Q30679157
Comparison of protein solution structures refined by molecular dynamics simulation in vacuum, with a generalized Born model, and with explicit waterQ32111367
Mapping the interaction surface of a membrane protein: unveiling the conformational switch of phospholamban in calcium pump regulation.Q33935080
An innovative procedure using a sublimable solid to align lipid bilayers for solid-state NMR studiesQ34177836
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(1)H/(15)N heteronuclear NMR spectroscopy shows four dynamic domains for phospholamban reconstituted in dodecylphosphocholine micelles.Q34186620
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Improving the quality of NMR and crystallographic protein structures by means of a conformational database potential derived from structure databasesQ36279917
Solution NMR of membrane proteins in bilayer mimics: small is beautiful, but sometimes bigger is betterQ36454928
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Transmembrane helix uniformity examined by spectral mapping of torsion angles.Q36731230
Isotropic bicelles stabilize the functional form of a small multidrug-resistance pump for NMR structural studiesQ36864826
Small multidrug resistance proteins: a multidrug transporter family that continues to grow.Q36973879
Structural and dynamic basis of phospholamban and sarcolipin inhibition of Ca(2+)-ATPaseQ37032523
Protein-protein interactions in the membrane: sequence, structural, and biological motifsQ37210616
Asymmetric methyl group labeling as a probe of membrane protein homo-oligomers by NMR spectroscopyQ37236029
United we stand: combining structural methodsQ37255416
High-resolution NMR spectroscopy of a GPCR in aligned bicellesQ39740463
Solid-state NMR reveals structural and dynamical properties of a membrane-anchored electron-carrier protein, cytochrome b5.Q42054317
Application of solid-state NMR restraint potentials in membrane protein modelingQ42146940
A structure refinement protocol combining NMR residual dipolar couplings and small angle scattering restraintsQ44689437
Serine 16 phosphorylation induces an order-to-disorder transition in monomeric phospholambanQ46377172
Structural fitting of PISEMA spectra of aligned proteinsQ46423370
De novo folding of membrane proteins: an exploration of the structure and NMR properties of the fd coat proteinQ47954872
Intensity and mosaic spread analysis from PISEMA tensors in solid-state NMR.Q51955914
Complete cross-validation and R-factor calculation of a solid-state NMR derived structure.Q52058332
Determination of three-dimensional structures of proteins by simulated annealing with interproton distance restraints. Application to crambin, potato carboxypeptidase inhibitor and barley serine proteinase inhibitor 2.Q52568701
Structural dynamics and topology of phospholamban in oriented lipid bilayers using multidimensional solid-state NMRQ57099285
Membrane-bound dimer structure of a beta-hairpin antimicrobial peptide from rotational-echo double-resonance solid-state NMRQ57099586
Experimental aspects of multidimensional solid-state NMR correlation spectroscopyQ57102590
Ez, a Depth-dependent Potential for Assessing the Energies of Insertion of Amino Acid Side-chains into Membranes: Derivation and Applications to Determining the Orientation of Transmembrane and Interfacial HelicesQ57837597
Improvements and Extensions in the Conformational Database Potential for the Refinement of NMR and X-ray Structures of Proteins and Nucleic AcidsQ57904759
Combining experimental information from crystal and solution studies: joint X-ray and NMR refinementQ57904841
A target function for quaternary structural refinement from small angle scattering and NMR orientational restraintsQ57912338
Assessing the effects of time and spatial averaging in 15N chemical shift/15N-1H dipolar correlation solid state NMR experimentsQ58423969
Solid-state NMR and rigid body molecular dynamics to determine domain orientations of monomeric phospholambanQ74587413
Two-dimensional solid-state NMR reveals two topologies of sarcolipin in oriented lipid bilayersQ80214751
Structure determination of membrane proteins by NMR spectroscopyQ80435122
P433issue4
P407language of work or nameEnglishQ1860
P921main subjectmembrane proteinQ423042
P304page(s)195-205
P577publication date2009-07-14
P1433published inJournal of Biomolecular NMRQ3186900
P1476titleA refinement protocol to determine structure, topology, and depth of insertion of membrane proteins using hybrid solution and solid-state NMR restraints.
P478volume44

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cites work (P2860)
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