Coupling DNA unwinding activity with primer synthesis in the bacteriophage T4 primosome

scientific article

Coupling DNA unwinding activity with primer synthesis in the bacteriophage T4 primosome is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1038/NCHEMBIO.236
P932PMC publication ID2784132
P698PubMed publication ID19838204
P5875ResearchGate publication ID38019351

P50authorVincent CroquetteQ3559559
Zhihao ZhuangQ73784616
P2093author name stringMaria Manosas
Michelle M Spiering
Stephen J Benkovic
P2860cites workArchitecture of the replication complex and DNA loops at the fork generated by the bacteriophage t4 proteinsQ73154934
Trading places on DNA--a three-point switch underlies primer handoff from primase to the replicative DNA polymeraseQ78170535
Interactions of bacteriophage T4-coded primase (gp61) with the T4 replication helicase (gp41) and DNA in primosome formationQ78239985
The oligomeric T4 primase is the functional form during replicationQ81752468
The control mechanism for lagging strand polymerase recycling during bacteriophage T4 DNA replicationQ82325512
Magnetic tweezers: micromanipulation and force measurement at the molecular levelQ24537528
Real-time observation of bacteriophage T4 gp41 helicase reveals an unwinding mechanismQ24669910
The primase active site is on the outside of the hexameric bacteriophage T7 gene 4 helicase-primase ringQ30307816
Reconstruction of bacteriophage T4 DNA replication apparatus from purified components: rolling circle replication following de novo chain initiation on a single-stranded circular DNA templateQ30828944
Assembly of the bacteriophage T4 helicase: architecture and stoichiometry of the gp41-gp59 complexQ31047098
Assembly of the bacteriophage T4 primosome: single-molecule and ensemble studiesQ33927559
Replisome-mediated DNA replication.Q33950250
A zinc ribbon protein in DNA replication: primer synthesis and macromolecular interactions by the bacteriophage T4 primaseQ34104288
DNA primase acts as a molecular brake in DNA replicationQ34490702
Single-molecule studies of fork dynamics in Escherichia coli DNA replicationQ37118273
Effects of the bacteriophage T4 gene 41 and gene 32 proteins on RNA primer synthesis: coupling of leading- and lagging-strand DNA synthesis at a replication forkQ38341960
Affinity and sequence specificity of DNA binding and site selection for primer synthesis by Escherichia coli primaseQ38359930
DNA replication with bacteriophage T4 proteins. Purification of the proteins encoded by T4 genes 41, 45, 44, and 62 using a complementation assayQ41049316
RNA primer handoff in bacteriophage T4 DNA replication: the role of single-stranded DNA-binding protein and polymerase accessory proteinsQ42347824
Stoichiometry and DNA unwinding by the bacteriophage T4 41:59 helicaseQ43443348
Bacteriophage T4 DNA primase-helicase. Characterization of oligomer synthesis by T4 61 protein alone and in conjunction with T4 41 proteinQ43906529
Distribution of growing points in DNa of bacteriophage T4.Q44456803
Purification and characterization of bacteriophage T4 gene 59 protein. A DNA helicase assembly protein involved in DNA replication.Q46080119
Accessory proteins function as matchmakers in the assembly of the T4 DNA polymerase holoenzymeQ49320874
The utility of a two-color fluorescence electrophoretic mobility shift assay procedure for the analysis of DNA replication complexes.Q50796151
Dissociative properties of the proteins within the bacteriophage T4 replisome.Q53650566
Studies of the DNA helicase-RNA primase unit from bacteriophage T4. A trinucleotide sequence on the DNA template starts RNA primer synthesisQ68960758
Trypsin cleavage in the COOH terminus of the bacteriophage T4 gene 41 DNA helicase alters the primase-helicase activities of the T4 replication complex in vitroQ69057589
Characterization of the bacteriophage T4 gene 41 DNA helicaseQ69334696
Two types of replication proteins increase the rate at which T4 DNA polymerase traverses the helical regions in a single-stranded DNA templateQ72087411
Characterization of the DNA-dependent GTPase activity of T4 gene 41 protein, an essential component of the T4 bacteriophage DNA replication apparatusQ72457053
P433issue12
P921main subjectbacteriophageQ165028
P304page(s)904-912
P577publication date2009-10-18
P1433published inNature Chemical BiologyQ904026
P1476titleCoupling DNA unwinding activity with primer synthesis in the bacteriophage T4 primosome
P478volume5

Reverse relations

cites work (P2860)
Q38534013A Replisome's journey through the bacterial chromosome
Q34236588A Specific Docking Site for DNA Polymerase α-Primase on the SV40 Helicase Is Required for Viral Primosome Activity, but Helicase Activity Is Dispensable
Q49604466A conserved structural element in the RNA helicase UPF1 regulates its catalytic activity in an isoform-specific manner
Q39285531A mechanistic study of helicases with magnetic traps
Q24610541Active and passive mechanisms of helicases
Q38095298Bacterial replication, transcription and translation: mechanistic insights from single-molecule biochemical studies
Q43526830Biochemistry: Molecular hurdles cleared with ease
Q30521445Collaborative coupling between polymerase and helicase for leading-strand synthesis
Q26991929Coordinated DNA Replication by the Bacteriophage T4 Replisome
Q58107303Correction-free force calibration for magnetic tweezers experiments
Q22121971DNA replication at the single-molecule level
Q35046681DNA unwinding by ring-shaped T4 helicase gp41 is hindered by tension on the occluded strand
Q36024282Dda helicase tightly couples translocation on single-stranded DNA to unwinding of duplex DNA: Dda is an optimally active helicase
Q37382539Direct observation of stalled fork restart via fork regression in the T4 replication system.
Q26998987Dynamic coupling between the motors of DNA replication: hexameric helicase, DNA polymerase, and primase
Q35007108E. coli DNA replication in the absence of free β clamps
Q34149339Efficient preparation of internally modified single-molecule constructs using nicking enzymes
Q47547792HTLV-1 Tax plugs and freezes UPF1 helicase leading to nonsense-mediated mRNA decay inhibition
Q61807894Hexameric helicase G40P unwinds DNA in single base pair steps
Q30658061Human Upf1 is a highly processive RNA helicase and translocase with RNP remodelling activities
Q37777437Insight into helicase mechanism and function revealed through single-molecule approaches
Q37175055Insights into Okazaki fragment synthesis by the T4 replisome: the fate of lagging-strand holoenzyme components and their influence on Okazaki fragment size
Q30436328Lab-on-a-chip technologies for single-molecule studies
Q38891864Magnetic tweezers for the study of DNA tracking motors.
Q30521444Mechanism of strand displacement synthesis by DNA replicative polymerases
Q64230099Mechanistic characterization of the DEAD-box RNA helicase Ded1 from yeast as revealed by a novel technique using single-molecule magnetic tweezers
Q38893312Modular magnetic tweezers for single-molecule characterizations of helicases
Q34103581Mutations altering the interplay between GkDnaC helicase and DNA reveal an insight into helicase unwinding
Q47172875Noise in the Machine: Alternative Pathway Sampling is the Rule During DNA Replication
Q30459218On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves
Q92223824Parallelized DNA tethered bead measurements to scrutinize DNA mechanical structure
Q36914672Pif1 is a force-regulated helicase
Q58930231Prime-time looping
Q35758114Probing DNA helicase kinetics with temperature-controlled magnetic tweezers
Q38784742RNA primer-primase complexes serve as the signal for polymerase recycling and Okazaki fragment initiation in T4 phage DNA replication
Q30545725RecG and UvsW catalyse robust DNA rewinding critical for stalled DNA replication fork rescue
Q38123787Replication-fork dynamics.
Q33698530Replisome mechanics: lagging strand events that influence speed and processivity
Q57752932Single molecule kinetics uncover roles for E. coli RecQ DNA helicase domains and interaction with SSB
Q37706259Single-molecule fluorescence reveals the unwinding stepping mechanism of replicative helicase.
Q24597472Single-molecule mechanical identification and sequencing
Q38860416Stalled replication fork rescue requires a novel DNA helicase
Q27660253Structure of a DNA Polymerase  -Primase Domain That Docks on the SV40 Helicase and Activates the Viral Primosome
Q38059632Studying genomic processes at the single-molecule level: introducing the tools and applications
Q34762664Substrate-selective repair and restart of replication forks by DNA translocases
Q27673737The hexameric helicase DnaB adopts a nonplanar conformation during translocation.
Q34973846The mini-chromosome maintenance (Mcm) complexes interact with DNA polymerase α-primase and stimulate its ability to synthesize RNA primers
Q39755005The rate of polymerase release upon filling the gap between Okazaki fragments is inadequate to support cycling during lagging strand synthesis
Q33911177Timing, coordination, and rhythm: acrobatics at the DNA replication fork.
Q39136293Tweezing of Magnetic and Non-Magnetic Objects with Magnetic Fields
Q46296300Understanding DNA replication by the bacteriophage T4 replisome
Q39040191Watching cellular machinery in action, one molecule at a time.

Search more.