Design and evaluation of a unique SYBR Green real-time RT-PCR assay for quantification of five major cytokines in cattle, sheep and goats

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Design and evaluation of a unique SYBR Green real-time RT-PCR assay for quantification of five major cytokines in cattle, sheep and goats is …
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scholarly articleQ13442814

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P6179Dimensions Publication ID1040255326
P356DOI10.1186/S12917-015-0382-0
P932PMC publication ID4369058
P698PubMed publication ID25889787
P5875ResearchGate publication ID273785000

P2093author name stringCarinne Puech
Isabelle Chantal
Laurence Dedieu
Valérie Rodrigues
P2860cites workTranscriptional profiling of immune genes in bovine monocyte-derived macrophages exposed to bacterial antigens.Q54371961
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TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional propertiesQ29547848
Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data setsQ29615465
Differential cytokine gene expression profiles in the three pathological forms of sheep paratuberculosisQ33294117
Innate gene repression associated with Mycobacterium bovis infection in cattle: toward a gene signature of diseaseQ33304580
A practical approach to RT-qPCR-Publishing data that conform to the MIQE guidelinesQ33538911
An overview of real-time quantitative PCR: applications to quantify cytokine gene expressionQ34532446
Pitfalls of quantitative real-time reverse-transcription polymerase chain reactionQ35872589
Cytokine gene expression in peripheral blood mononuclear cells and tissues of cattle infected with Mycobacterium avium subsp. paratuberculosis: evidence for an inherent proinflammatory gene expression patternQ36575764
Reference gene selection for quantitative PCR studies in sheep neutrophilsQ37007182
A current perspective on availability of tools, resources and networks for veterinary immunologyQ37332495
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Impact of delayed processing of bovine peripheral blood on differential gene expressionQ38499609
Evaluation of internal reference genes for quantitative expression analysis by real-time PCR in ovine whole blood.Q39675248
The validation of housekeeping genes as a reference in quantitative Real Time PCR analysis: application in the milk somatic cells and frozen whole blood of goats infected with caprine arthritis encephalitis virus.Q42203402
New technologies for studying immune regulation in ruminantsQ43487140
Cytokine mRNA expression in cattle infected with different dosages of Mycobacterium bovisQ43546647
Expression profiles of different cytokine genes in peripheral blood mononuclear cells of goats infected experimentally with native strain of Mycobacterium avium subsp. paratuberculosisQ43787202
Identification of internal control genes for quantitative expression analysis by real-time PCR in bovine peripheral lymphocytes.Q44933700
Enhanced proinflammatory cytokine activity during experimental bluetongue virus-1 infection in Indian native sheepQ45361438
Quantitation of ovine cytokine mRNA by real-time RT-PCR.Q46928398
Development of detection methods for ruminant interleukin (IL)-12.Q47569242
Development of an ELISA for bovine IL-10.Q47571174
Development of detection methods for ruminant interleukin (IL)-4.Q47615209
Production and characterization of two monoclonal antibodies to bovine tumour necrosis factor alpha (TNF-alpha) and their cross-reactivity with ovine TNF-alpha.Q47618527
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P407language of work or nameEnglishQ1860
P304page(s)65
P577publication date2015-03-17
P1433published inBMC Veterinary ResearchQ15751340
P1476titleDesign and evaluation of a unique SYBR Green real-time RT-PCR assay for quantification of five major cytokines in cattle, sheep and goats
P478volume11

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