Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus

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Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus is …
instance of (P31):
scholarly articleQ13442814

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P6179Dimensions Publication ID1044047861
P356DOI10.1186/1471-2199-14-17
P932PMC publication ID3751577
P698PubMed publication ID23919831
P5875ResearchGate publication ID255693123

P50authorJoanna ŻebrowskaQ62653320
Joanna Jeżewska-FrąckowiakQ82808279
Agnieszka Zylicz-StachulaQ92415169
Piotr SkowronQ62027585
P2093author name stringArvydas Lubys
Danute Ramanauskaite
Goda Mitkaite
Jolanta Vitkute
P2860cites workChemically-induced affinity star restriction specificity: a novel TspGWI/sinefungin endonuclease with theoretical 3-bp cleavage frequencyQ84585360
Characterization and crystal structure of the type IIG restriction endonuclease RM.BpuSIQ27670651
Structure of restriction endonuclease bamhi phased at 1.95 A resolution by MAD analysisQ27730914
Related bifunctional restriction endonuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsoI with distinct specificitiesQ31054232
Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermus sp. enzyme familyQ33456218
Genome comparison and context analysis reveals putative mobile forms of restriction-modification systems and related rearrangementsQ33783220
Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving.Q34091757
A new method for the rapid identification of genes encoding restriction and modification enzymesQ35761450
Diversity of DNA methyltransferases that recognize asymmetric target sequencesQ37698622
Class-IIS restriction enzymes--a reviewQ37765759
TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5'-ACGGA(N11/9)-3'.Q39538514
A new Thermus sp. class-IIS enzyme sub-family: isolation of a 'twin' endonuclease TspDTI with a novel specificity 5'-ATGAA(N(11/9))-3', related to TspGWI, TaqII and Tth111II.Q39798515
A second type II restriction endonuclease from Thermus aquaticus with an unusual sequence specificity.Q40461065
Nucleotide sequence of the BsuRI restriction-modification system.Q40471693
A second site specific endonuclease from Thermus thermophilus 111, Tth111IIQ40481824
Rapid shotgun cloning utilizing the two base recognition endonuclease CviJI.Q40533180
Improvement of the strain for the rapid identification of genes encoding restriction and modification enzymes.Q54646241
Cloning the modification methylase gene of Bacillus sphaericus R in Escherichia coliQ60505834
P921main subjectThermus scotoductusQ26297002
P304page(s)17
P577publication date2013-08-06
P1433published inBMC Molecular BiologyQ15760954
P1476titleThree-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus
P478volume14

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cites work (P2860)
Q52881851A putative Type IIS restriction endonuclease GeoICI from Geobacillus sp.--A robust, thermostable alternative to mezophilic prototype BbvI.
Q41887023Characterization of cleavage intermediate and star sites of RM.Tth111II.
Q38544663Cofactor analogue-induced chemical reactivation of endonuclease activity in a DNA cleavage/methylation deficient TspGWI N₄₇₃A variant in the NPPY motif
Q50879383Engineering TaqII bifunctional endonuclease DNA recognition fidelity: the effect of a single amino acid substitution within the methyltransferase catalytic site.
Q41874787Modified 'one amino acid-one codon' engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase
Q64084068Novel parameter describing restriction endonucleases: Secondary-Cognate-Specificity and chemical stimulation of TsoI leading to substrate specificity change
Q41626758The third restriction-modification system from Thermus aquaticus YT-1: solving the riddle of two TaqII specificities.
Q99634713Type II Restriction-Modification System from Gardnerella vaginalis ATCC 14018

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