Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications

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Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications is …
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scholarly articleQ13442814

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P6179Dimensions Publication ID1049334553
P356DOI10.1186/1472-6750-7-76
P932PMC publication ID2213645
P698PubMed publication ID17996102
P5875ResearchGate publication ID5850989

P2093author name stringFei Mao
Xing Xin
Wai-Yee Leung
P2860cites workApplication of the DNA-specific dye EvaGreen for the routine quantification of DNA in microplatesQ80234846
Faster quantitative real-time PCR protocols may lose sensitivity and show increased variabilityQ24536294
Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implicationsQ24563152
Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella/mammalian microsome reverse mutation assay (Ames test)Q28137696
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assaysQ28140044
Simultaneous amplification and detection of specific DNA sequencesQ28185655
Amplicon melting analysis with labeled primers: a closed-tube method for differentiating homozygotes and heterozygotesQ28211431
Faster, simpler, more-specific methods for improved molecular detection of Phytophthora ramorum in the fieldQ28298799
Product differentiation by analysis of DNA melting curves during the polymerase chain reactionQ28305051
Real time quantitative PCRQ29616373
Genetic mutation analysis by CE with LIF detection using inverse-flow derivatization of DNA fragmentsQ33255780
Ethidium bromide and SYBR Green I enhance the genotoxicity of UV-irradiation and chemical mutagens in E. coliQ33948572
Amplification efficiency of thermostable DNA polymerasesQ33972753
Combining nucleic acid amplification and detectionQ34134912
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.Q34146463
High-resolution genotyping by amplicon melting analysis using LCGreenQ34199727
Real-time PCR detection of Brucella abortus: a comparative study of SYBR green I, 5'-exonuclease, and hybridization probe assaysQ35210597
Enhancement of abscisic acid sensitivity and reduction of water consumption in Arabidopsis by combined inactivation of the protein phosphatases type 2C ABI1 and HAB1.Q39419425
A new minor groove binding asymmetric cyanine reporter dye for real-time PCR.Q39747890
Demonstration of preferential binding of SYBR Green I to specific DNA fragments in real-time multiplex PCRQ40240947
Novel mfgl2 antisense plasmid inhibits murine fgl2 expression and ameliorates murine hepatitis virus type 3-induced fulminant hepatitis in BALB/cJ miceQ40267642
A rapid detection of PCR amplification product using a new fluorescent intercalator; the pyrylium dye, P2.Q40393809
Evaluation of a homemade SYBR green I reaction mixture for real-time PCR quantification of gene expressionQ43180859
DNA melting in the presence of fluorescent intercalating oxazole yellow dyes measured with a gel-based assayQ43791450
Real-time RT-PCR and SYBR Green I melting curve analysis for the identification of Plum pox virus strains C, EA, and W: effect of amplicon size, melt rate, and dye translocationQ44753708
Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridizationQ46960543
Evaluation of post-polymerase chain reaction melting temperature analysis for meat species identification in mixed DNA samples.Q50712332
Capillary electrophoresis of double-stranded DNA fragments using a new fluorescence intercalating dye EvaGreen.Q50723861
Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis.Q50773308
An integrated fluorescence detection system for lab-on-a-chip applications.Q51582587
DNA quantification using EvaGreen and a real-time PCR instrument.Q54595392
DNA melting analysis for detection of single nucleotide polymorphismsQ60628077
Binding of ethidium bromide to double-stranded ribonucleic acidQ69380100
Binding of symmetrical cyanine dyes into the DNA minor grooveQ73047962
Effects of ethidium bromide and SYBR Green I on different polymerase chain reaction systemsQ73385512
P304page(s)76
P577publication date2007-11-09
P1433published inBMC BiotechnologyQ15753225
P1476titleCharacterization of EvaGreen and the implication of its physicochemical properties for qPCR applications
P478volume7

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