Purification of polyethylenimine polyplexes highlights the role of free polycations in gene transfer

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Purification of polyethylenimine polyplexes highlights the role of free polycations in gene transfer is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1002/JGM.598
P8608Fatcat IDrelease_kpgb7ceyyzfnxhsbkagfrpk4tq
P698PubMed publication ID15386739
P5875ResearchGate publication ID8327755

P50authorErnst WagnerQ39051317
P2093author name stringKatharina von Gersdorff
Manfred Ogris
Carsten Culmsee
Sabine Boeckle
Silke van der Piepen
P433issue10
P304page(s)1102-1111
P577publication date2004-10-01
P1433published inJournal of Gene MedicineQ15746377
P1476titlePurification of polyethylenimine polyplexes highlights the role of free polycations in gene transfer
P478volume6

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cites work (P2860)
Q38799201A Cell-Penetrating Peptide with a Guanidinylethyl Amine Structure Directed to Gene Delivery
Q51748614A mechanistic dissection of polyethylenimine mediated transfection of CHO cells: to enhance the efficiency of recombinant DNA utilization.
Q37157373A nanoparticle formulation that selectively transfects metastatic tumors in mice
Q41876243A polyethylenimine-linoleic acid conjugate for antisense oligonucleotide delivery
Q34479518A truncated HGP peptide sequence that retains endosomolytic activity and improves gene delivery efficiencies
Q24794521A versatile reducible polycation-based system for efficient delivery of a broad range of nucleic acids
Q39136603AAV2 production with optimized N/P ratio and PEI-mediated transfection results in low toxicity and high titer for in vitro and in vivo applications
Q38541348Advancing polymeric delivery systems amidst a nucleic acid therapy renaissance
Q39654791Amine-modified poly(vinyl alcohol)s as non-viral vectors for siRNA delivery: effects of the degree of amine substitution on physicochemical properties and knockdown efficiency
Q46408570An acid sensitive ketal-based polyethylene glycol-oligoethylenimine copolymer mediates improved transfection efficiency at reduced toxicity
Q39321848Application of siRNA against SARS in the rhesus macaque model
Q29248141Barriers to inhaled gene therapy of obstructive lung diseases: A review
Q35884341Biodegradable poly(2-dimethylamino ethylamino)phosphazene for in vivo gene delivery to tumor cells. Effect of polymer molecular weight
Q34072540Biophysical characterization of copolymer-protected gene vectors.
Q37763677Bioresponsive polymers for the delivery of therapeutic nucleic acids.
Q30560846Cationic lipid-coated PEI/DNA polyplexes with improved efficiency and reduced cytotoxicity for gene delivery into mesenchymal stem cells
Q54259697Cationic polymers for non-viral gene delivery to human T cells.
Q36771471Cell and tissue targeting of nucleic acids for cancer gene therapy
Q58322949Chemistry specificity of DNA–polycation complex salt response: a simulation study of DNA, polylysine and polyethyleneimine
Q38122934Chitosans for delivery of nucleic acids.
Q33399696Combinatorial evaluation of cations, pH-sensitive and hydrophobic moieties for polymeric vector design
Q41585561Comparative study of guanidine-based and lysine-based brush copolymers for plasmid delivery
Q39651726Controlled synthesis of PEI-coated gold nanoparticles using reductive catechol chemistry for siRNA delivery
Q37557225Crosslinked linear polyethylenimine enhances delivery of DNA to the cytoplasm
Q28659480Current progress in gene delivery technology based on chemical methods and nano-carriers
Q45870434Decationized polyplexes for gene delivery.
Q38356303Delivery of CD44 shRNA/nanoparticles within cancer cells: perturbation of hyaluronan/CD44v6 interactions and reduction in adenoma growth in Apc Min/+ MICE.
Q57610427Dendrimer-Based Gene Delivery Systems: Administration Routes andIn VivoEvaluation
Q42723857Differential intracellular distribution of DNA complexed with polyethylenimine (PEI) and PEI-polyarginine PTD influences exogenous gene expression within live COS-7 cells
Q39530004Disconnecting the yin and yang relation of epidermal growth factor receptor (EGFR)-mediated delivery: a fully synthetic, EGFR-targeted gene transfer system avoiding receptor activation
Q39616606Disulfide-based poly(amido amine)s for siRNA delivery: effects of structure on siRNA complexation, cellular uptake, gene silencing and toxicity
Q39631310Di‐ and Triblock siRNA‐PEG Copolymers: PEG Density Effect of Polyelectrolyte Complexes on Cellular Uptake and Gene Silencing Efficiency
Q30493541Drug nanocarriers labeled with near-infrared-emitting quantum dots (quantoplexes): imaging fast dynamics of distribution in living animals
Q58483540Effects of hypoxia and limited diffusion in tumor cell microenvironment on bystander effect of P450 prodrug therapy
Q33862935Elucidating the role of free polycationic chains in polycation gene carriers by free chains of polyethylenimine or N,N,N-trimethyl chitosan plus a certain polyplex
Q61703770Enhanced Lung Gene Expression After Aerosol Delivery of Concentrated pDNA/PEI Complexes
Q58703541Enhanced gene transfection efficiency by low-dose 25 kDa polyethylenimine by the assistance of 1.8 kDa polyethylenimine
Q39173256Enhanced transfection by antioxidative polymeric gene carrier that reduces polyplex-mediated cellular oxidative stress
Q58560469Enhanced transfection of a macromolecular lignin-based DNA complex with low cellular toxicity
Q56894131Evaluation of the transfection property of a peptide ligand for the fibroblast growth factor receptor as part of PEGylated polyethylenimine polyplex
Q36555626Exploring the role of polymer structure on intracellular nucleic acid delivery via polymeric nanoparticles.
Q41444330Formulation of a peptide nucleic acid based nucleic acid delivery construct
Q64241011Free Polyethylenimine Enhances Substrate-Mediated Gene Delivery on Titanium Substrates Modified With RGD-Functionalized Poly(acrylic acid) Brushes
Q82489428Gene expression profiles in mouse lung tissue after administration of two cationic polymers used for nonviral gene delivery
Q34756391Generation of a tumor- and tissue-specific episomal non-viral vector system
Q43419330Generation of magnetic nonviral gene transfer agents and magnetofection in vitro
Q51637633High DNA-Binding Affinity and Gene-Transfection Efficacy of Bioreducible Cationic Nanomicelles with a Fluorinated Core.
Q38541526Highlighting the role of polymer length, carbohydrate size, and nucleic acid type in potency of glycopolycation agents for pDNA and siRNA delivery
Q38759514History of Polymeric Gene Delivery Systems
Q57610433Hydration forces as a tool for the optimization of core–shell nanoparticle vectors for cancer gene therapy
Q51759826Hyperbranched polylysine: a versatile, biodegradable transfection agent for the production of recombinant proteins by transient gene expression and the transfection of primary cells.
Q33279955Identification of novel superior polycationic vectors for gene delivery by high-throughput synthesis and screening of a combinatorial library
Q41987139Influence of the molecular weight of bioreducible oligoethylenimine conjugates on the polyplex transfection properties
Q86486121Insight into polycation chain length affecting transfection efficiency by O-methyl-free N,N,N-trimethyl chitosans as gene carriers
Q34594717Interactions of a charged nanoparticle with a lipid membrane: implications for gene delivery
Q36497229Interfering antiviral immunity: application, subversion, hope?
Q36902000Investigation of Polyethylenimine/DNA Polyplex Transfection to Cultured Cells Using Radiolabeling and Subcellular Fractionation Methods.
Q90019882Kinetic Control in Assembly of Plasmid DNA/Polycation Complex Nanoparticles
Q39565694Layer-by-layer coated gold nanoparticles: size-dependent delivery of DNA into cells.
Q37425606Lipid-based systemic delivery of siRNA.
Q35846845Low molecular weight chitosan nanoparticulate system at low N:P ratio for nontoxic polynucleotide delivery.
Q37633436Lung gene therapy with highly compacted DNA nanoparticles that overcome the mucus barrier
Q51766886Mechanisms of polyethylenimine-mediated DNA delivery: free carrier helps to overcome the barrier of cell-surface glycosaminoglycans.
Q41566501Metabolically stabilized long-circulating PEGylated polyacridine peptide polyplexes mediate hydrodynamically stimulated gene expression in liver.
Q42818425Microfluidic Assembly of Cationic-β-Cyclodextrin:Hyaluronic Acid-Adamantane Host:Guest pDNA Nanoparticles
Q35911766Missing pieces in understanding the intracellular trafficking of polycation/DNA complexes
Q39122756Molecular logistics using cytocleavable polyrotaxanes for the reactivation of enzymes delivered in living cells
Q41760723Non-viral gene therapy that targets motor neurons in vivo
Q40067273Novel biocompatible cationic copolymers based on polyaspartylhydrazide being potent as gene vector on tumor cells
Q45872167Novel degradable oligoethylenimine acrylate ester-based pseudodendrimers for in vitro and in vivo gene transfer
Q26851886Nucleic acid delivery: the missing pieces of the puzzle?
Q38027844Nucleic acid therapeutics: concepts for targeted delivery to solid tumors
Q58384820Opposites attract: influence of the molar mass of branched poly(ethylene imine) on biophysical characteristics of siRNA-based polyplexese
Q42237653Optimization of brush-like cationic copolymers for nonviral gene delivery
Q28476268Optimizing targeted gene delivery: chemical modification of viral vectors and synthesis of artificial virus vector systems
Q39540005PEGylation improves nanoparticle formation and transfection efficiency of messenger RNA.
Q85193035Poly[(5-methyl-5-allyloxycarbonyl-trimethylene carbonate)-co-(5,5-dimethyl-trimethylene carbonate)] with Grafted Polyethylenimine as Biodegradable Polycations for Efficient Gene Delivery
Q39721514Polycation-induced cell membrane permeability does not enhance cellular uptake or expression efficiency of delivered DNA.
Q43448738Polyethyleneimine-poly(ethylene glycol)-star-copolymers as efficient and biodegradable vectors for mammalian cell transfection
Q34805862Polyethylenimine/oligonucleotide polyplexes investigated by fluorescence resonance energy transfer and fluorescence anisotropy
Q40152815Polymer functionalized gold nanoparticles as nonviral gene delivery reagents.
Q39169193Polyplex Evolution: Understanding Biology, Optimizing Performance
Q42251940Pulsed interleaved excitation
Q34165321Recent In Vivo Evidences of Particle-Based Delivery of Small-Interfering RNA (siRNA) into Solid Tumors
Q38074465Recent developments and perspectives on gene therapy using synthetic vectors
Q35618869Reducible HPMA-co-oligolysine copolymers for nucleic acid delivery
Q39106943Reversal of lung cancer multidrug resistance by pH-responsive micelleplexes mediating co-delivery of siRNA and paclitaxel
Q51760334Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells.
Q57343224Salt effects on macrophase separations in non-stoichiometric mixtures of oppositely charged macromolecules: Theory and experiment
Q33907953Self-assembled lipid nanomedicines for siRNA tumor targeting.
Q42921833Solid-phase supported design of carriers for therapeutic nucleic acid delivery
Q39891354Structural and functional consequences of poly(ethylene glycol) inclusion on DNA condensation for gene delivery
Q45249031Structural investigations of DNA-polycation complexes
Q36339369Subcellular trafficking and transfection efficacy of polyethylenimine-polyethylene glycol polyplex nanoparticles with a ligand to melanocortin receptor-1.
Q40340178Surface adsorption of DNA to tissue engineering scaffolds for efficient gene delivery
Q35041201Surface immobilization of plasmid DNA with a cell-responsive tether for substrate-mediated gene delivery
Q36041260Synthesis and application of poly(ethylene glycol)-co-poly(β-amino ester) copolymers for small cell lung cancer gene therapy
Q41852160Synthesis and evaluation of cyclic cationic polymers for nucleic acid delivery
Q45888639Targeted gene delivery to cancer cells with nanometric DNA particles enveloped with folic acid using a polymerisable anchor
Q36293332Targeting of polyplexes: toward synthetic virus vector systems
Q57376717Ternary complexes DNA–polyethylenimine–Fe(iii) with linear and branched polycations: implications on condensation, size, charge and in vitro biocompatibility
Q33999411The effect of N/P ratio on the in vitro and in vivo interaction properties of PEGylated poly[2-(dimethylamino)ethyl methacrylate]-based siRNA complexes
Q40231070The internalization route resulting in successful gene expression depends on both cell line and polyethylenimine polyplex type
Q46548778The kinetics of polyethylenimine-mediated transfection in suspension cultures of Chinese hamster ovary cells
Q33677061The mechanism of polyplex internalization into cells: testing the GM1/caveolin-1 lipid raft mediated endocytosis pathway
Q42541130The possible "proton sponge " effect of polyethylenimine (PEI) does not include change in lysosomal pH.
Q35300626The transduction of Coxsackie and Adenovirus Receptor-negative cells and protection against neutralizing antibodies by HPMA-co-oligolysine copolymer-coated adenovirus
Q57342777Thermoresponsive Gene Carriers Based on Polyethylenimine-graft-Poly[oligo(ethylene glycol) methacrylate]
Q33985556Transfection of rat pancreatic islet tissue by polymeric gene vectors
Q42606717Transient transfection of CHO cells using linear polyethylenimine is a simple and effective means of producing rainbow trout recombinant IFN-γ protein
Q39758973Tuning physical properties of nanocomplexes through microfluidics-assisted confinement
Q57822658Ultra-small graphene oxide functionalized with polyethylenimine (PEI) for very efficient gene delivery in cell and zebrafish embryos
Q38719519Use of a protein engineering strategy to overcome limitations in the production of "Difficult to Express" recombinant proteins
Q39362979Using siRNA in prophylactic and therapeutic regimens against SARS coronavirus in Rhesus macaque
Q34700581Visualizing the attack of RNase enzymes on dendriplexes and naked RNA using atomic force microscopy
Q36684451Well-defined cross-linked antioxidant nanozymes for treatment of ischemic brain injury

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