Scm3 deposits a (Cse4-H4)2 tetramer onto DNA through a Cse4-H4 dimer intermediate

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Scm3 deposits a (Cse4-H4)2 tetramer onto DNA through a Cse4-H4 dimer intermediate is …
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scholarly articleQ13442814

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P356DOI10.1093/NAR/GKU205
P932PMC publication ID4027189
P698PubMed publication ID24623811
P5875ResearchGate publication ID260761213

P50authorKarolin LugerQ6373153
P2093author name stringMekonnen Lemma Dechassa
Katharina Wyns
P2860cites workCrystal structure of the nucleosome core particle at 2.8 A resolutionQ22122355
Dimerization of the CENP-A assembly factor HJURP is required for centromeric nucleosome depositionQ24295179
Structure of a CENP-A-histone H4 heterodimer in complex with chaperone HJURPQ24299299
Centromere-specific assembly of CENP-a nucleosomes is mediated by HJURP.Q24321321
HJURP is a cell-cycle-dependent maintenance and deposition factor of CENP-A at centromeresQ24321377
Structure of the yeast nucleosome core particle reveals fundamental changes in internucleosome interactionsQ24535791
The structure of (CENP-A-H4)(2) reveals physical features that mark centromeresQ24599635
Structural basis for recognition of centromere histone variant CenH3 by the chaperone Scm3Q27667279
Recognition of the centromere-specific histone Cse4 by the chaperone Scm3Q27667962
Crystal structure of the human centromeric nucleosome containing CENP-AQ27670750
Identification of Functionally Conserved Regions in the Structure of the Chaperone/CenH3/H4 ComplexQ27675219
High-resolution mapping of changes in histone-DNA contacts of nucleosomes remodeled by ISW2Q27930945
Histone chaperone specificity in Rtt109 activationQ27931083
Scm3, an essential Saccharomyces cerevisiae centromere protein required for G2/M progression and Cse4 localizationQ27932322
A mutation in CSE4, an essential gene encoding a novel chromatin-associated protein in yeast, causes chromosome nondisjunction and cell cycle arrest at mitosisQ27936451
Nonhistone Scm3 and histones CenH3-H4 assemble the core of centromere-specific nucleosomesQ27936719
The budding yeast point centromere associates with two Cse4 molecules during mitosis.Q27936860
Scm3 is essential to recruit the histone h3 variant cse4 to centromeres and to maintain a functional kinetochoreQ27939322
Cse4 is part of an octameric nucleosome in budding yeastQ27939957
Histone H3 localizes to the centromeric DNA in budding yeastQ27940022
The human CENP-A centromeric nucleosome-associated complexQ28235264
Tetrameric structure of centromeric nucleosomes in interphase Drosophila cellsQ28469268
Assembly of Drosophila centromeric chromatin proteins during mitosisQ28478120
Fission yeast Scm3: A CENP-A receptor required for integrity of subkinetochore chromatinQ28752491
Centromeres and kinetochores: from epigenetics to mitotic checkpoint signalingQ29547850
Esperanto for histones: CENP-A, not CenH3, is the centromeric histone H3 variantQ30419902
In vitro centromere and kinetochore assembly on defined chromatin templates.Q30427862
HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochoreQ30428662
Double-strand DNA breaks recruit the centromeric histone CENP-A.Q30436771
Domain architectures of the Scm3p protein provide insights into centromere function and evolutionQ33294405
Efficient yeast ChIP-Seq using multiplex short-read DNA sequencingQ33401685
HJURP binds CENP-A via a highly conserved N-terminal domain and mediates its deposition at centromeresQ33667469
"Point" centromeres of Saccharomyces harbor single centromere-specific nucleosomesQ42561272
A thermodynamic model for Nap1-histone interactionsQ43242877
Octameric CENP-A nucleosomes are present at human centromeres throughout the cell cycleQ44974271
Incorporation of Drosophila CID/CENP-A and CENP-C into centromeres during early embryonic anaphaseQ47072785
Nucleotide sequence comparisons and functional analysis of yeast centromere DNAsQ48404737
Nonhistone Scm3 binds to AT-rich DNA to organize atypical centromeric nucleosome of budding yeast.Q53227972
Sulfyhydryl-Reactive Site-Directed Cross-Linking as a Method for Probing the Tetrameric Structure of Histones H3 and H4Q60489218
New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioningQ74352513
Quantifying chromatin-associated interactions: the HI-FI systemQ84838486
Expression and purification of recombinant histones and nucleosome reconstitutionQ33914576
Epigenetic analysis of kinetochore assembly on variant human centromeresQ34240260
Cdk activity couples epigenetic centromere inheritance to cell cycle progression.Q34240608
Reconstitution of nucleosome core particles from recombinant histones and DNA.Q34296889
The octamer is the major form of CENP-A nucleosomes at human centromeresQ34342975
Chaperone Nap1 shields histone surfaces used in a nucleosome and can put H2A-H2B in an unconventional tetrameric form.Q34366314
Chaperone-mediated assembly of centromeric chromatin in vitroQ34522243
Biophysical characterization of the centromere-specific nucleosome from budding yeastQ34537134
A NASP (N1/N2)-related protein, Sim3, binds CENP-A and is required for its deposition at fission yeast centromeres.Q34730114
Scm3 is a centromeric nucleosome assembly factorQ34752031
The histone chaperones Nap1 and Vps75 bind histones H3 and H4 in a tetrameric conformationQ34978251
Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null miceQ35008528
Structure and Scm3-mediated assembly of budding yeast centromeric nucleosomesQ35038924
H3.3 is deposited at centromeres in S phase as a placeholder for newly assembled CENP-A in G₁ phaseQ35079154
The activity of the histone chaperone yeast Asf1 in the assembly and disassembly of histone H3/H4-DNA complexes.Q35120741
Human centromere protein A (CENP-A) can replace histone H3 in nucleosome reconstitution in vitroQ35156261
CENP-C recruits M18BP1 to centromeres to promote CENP-A chromatin assemblyQ35514331
Fluorescence strategies for high-throughput quantification of protein interactionsQ35823643
Histone chaperone FACT coordinates nucleosome interaction through multiple synergistic binding eventsQ35842262
Perfect and imperfect nucleosome positioning in yeastQ35980831
Centromere identity is specified by a single centromeric nucleosome in budding yeastQ35990276
Propagation of centromeric chromatin requires exit from mitosis.Q36117994
A 17-kD centromere protein (CENP-A) copurifies with nucleosome core particles and with histonesQ36215978
Yeast CAF-1 assembles histone (H3-H4)2 tetramers prior to DNA depositionQ36368830
CAF-1-induced oligomerization of histones H3/H4 and mutually exclusive interactions with Asf1 guide H3/H4 transitions among histone chaperones and DNA.Q36478043
Cell-cycle-dependent structural transitions in the human CENP-A nucleosome in vivoQ36669215
Assembly in G1 phase and long-term stability are unique intrinsic features of CENP-A nucleosomesQ36717384
A 125-base-pair CEN6 DNA fragment is sufficient for complete meiotic and mitotic centromere functions in Saccharomyces cerevisiaeQ36758788
CENP-A confers a reduction in height on octameric nucleosomesQ36912565
Fission yeast Scm3 mediates stable assembly of Cnp1/CENP-A into centromeric chromatinQ37180182
Epigenetic inheritance of centromeresQ37806861
Nucleosome formation activity of human somatic nuclear autoantigenic sperm protein (sNASP).Q38346358
Preparation of nucleosome core particle from recombinant histonesQ40947701
Cell-cycle-coupled structural oscillation of centromeric nucleosomes in yeastQ41811815
Centromeric nucleosomes induce positive DNA supercoilsQ42113262
P275copyright licenseCreative Commons Attribution-NonCommercial 3.0 UnportedQ18810331
P6216copyright statuscopyrightedQ50423863
P433issue9
P407language of work or nameEnglishQ1860
P304page(s)5532-5542
P577publication date2014-03-12
P1433published inNucleic Acids ResearchQ135122
P1476titleScm3 deposits a (Cse4-H4)2 tetramer onto DNA through a Cse4-H4 dimer intermediate
P478volume42

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cites work (P2860)
Q58088567Conformational flexibility of histone variant CENP-A regulated by histone H4: a mechanism to stabilise soluble Cse4
Q39236417Histone H4 Facilitates the Proteolysis of the Budding Yeast CENP-ACse4 Centromeric Histone Variant.
Q37656813Histone chaperone networks shaping chromatin function
Q33795322Imaging the fate of histone Cse4 reveals de novo replacement in S phase and subsequent stable residence at centromeres
Q37397394In Vitro Chromatin Assembly: Strategies and Quality Control.
Q89808313The ATAD2/ANCCA homolog Yta7 cooperates with Scm3HJURP to deposit Cse4CENP-A at the centromere in yeast
Q34931192The centromere: epigenetic control of chromosome segregation during mitosis

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