Directly probing the mechanical properties of the spindle and its matrix

scientific article

Directly probing the mechanical properties of the spindle and its matrix is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1083/JCB.200907110
P932PMC publication ID2828919
P698PubMed publication ID20176922
P5875ResearchGate publication ID41532332

P50authorGaudenz DanuserQ60658035
P2093author name stringTimothy J Mitchison
Edward D Salmon
Jesse C Gatlin
Alexandre Matov
P2860cites workSpatiotemporal control of mitosis by the conserved spindle matrix protein MegatorQ24319051
Formation of spindle poles by dynein/dynactin-dependent transport of NuMAQ24670127
Dynein/dynactin regulate metaphase spindle length by targeting depolymerizing activities to spindle polesQ25257678
Yeast kinesin-8 depolymerizes microtubules in a length-dependent mannerQ27939784
Mouvement brownien d'un ellipsoide - I. Dispersion diélectrique pour des molécules ellipsoidalesQ29011621
Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extractsQ29616137
Micromanipulation studies of chromosome movement. I. Chromosome-spindle attachment and the mechanical properties of chromosomal spindle fibersQ30442441
Regional variation of microtubule flux reveals microtubule organization in the metaphase meiotic spindleQ30483135
Spindle fusion requires dynein-mediated sliding of oppositely oriented microtubulesQ30488756
Spinning disk confocal microscope system for rapid high-resolution, multimode, fluorescence speckle microscopy and green fluorescent protein imaging in living cellsQ31134384
Roles of polymerization dynamics, opposed motors, and a tensile element in governing the length of Xenopus extract meiotic spindlesQ33841491
Antagonistic microtubule-sliding motors position mitotic centrosomes in Drosophila early embryosQ33880076
Mechanical properties of Xenopus egg cytoplasmic extracts.Q34188623
Mitosis, microtubules, and the matrixQ34318145
A mitotic lamin B matrix induced by RanGTP required for spindle assemblyQ34502865
Cell cycle extractsQ34590228
Slide-and-cluster models for spindle assemblyQ34663861
A new method reveals microtubule minus ends throughout the meiotic spindleQ36118983
Spindle microtubules and their mechanical associations after micromanipulation in anaphaseQ36207064
Anaphase A chromosome movement and poleward spindle microtubule flux occur At similar rates in Xenopus extract spindlesQ36255338
Skeletor, a novel chromosomal protein that redistributes during mitosis provides evidence for the formation of a spindle matrixQ36293680
Eg5 is static in bipolar spindles relative to tubulin: evidence for a static spindle matrixQ36294036
The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindlesQ36322575
Direct observation of microtubule dynamics at kinetochores in Xenopus extract spindles: implications for spindle mechanicsQ36322983
Modeling mitosisQ36365798
Poleward microtubule flux mitotic spindles assembled in vitroQ36529365
Poleward transport of Eg5 by dynein-dynactin in Xenopus laevis egg extract spindlesQ36843155
Mechanisms of mitotic spindle assembly and functionQ37085042
Synchronizing chromosome segregation by flux-dependent force equalization at kinetochores.Q37267720
Dynein antagonizes eg5 by crosslinking and sliding antiparallel microtubulesQ37440564
Megator, an essential coiled-coil protein that localizes to the putative spindle matrix during mitosis in Drosophila.Q37594854
Dynein, Lis1 and CLIP-170 counteract Eg5-dependent centrosome separation during bipolar spindle assembly.Q39914545
Length control of the metaphase spindleQ40348496
Probing the mechanical architecture of the vertebrate meiotic spindle.Q42591440
Compression regulates mitotic spindle length by a mechanochemical switch at the polesQ43076243
The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitroQ46699281
Titin in insect spermatocyte spindle fibers associates with microtubules, actin, myosin and the matrix proteins skeletor, megator and chromator.Q50467735
Poly(ADP-ribose) is required for spindle assembly and structure.Q50668611
Mitosis: spindle evolution and the matrix model.Q51789282
Chromosome micromanipulation. I. The mechanics of chromosome attachment to the spindle.Q52494481
Architectural dynamics of the meiotic spindle revealed by single-fluorophore imaging.Q53522078
NuMA is a component of an insoluble matrix at mitotic spindle polesQ74658920
Fluorescent speckle microscopy of spindle microtubule assembly and motility in living cellsQ77801943
P433issue4
P407language of work or nameEnglishQ1860
P1104number of pages9
P304page(s)481-489
P577publication date2010-02-01
P1433published inJournal of Cell BiologyQ1524550
P1476titleDirectly probing the mechanical properties of the spindle and its matrix
P478volume188

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cites work (P2860)
Q37718329A computational model for the formation of lamin-B mitotic spindle envelope and matrix.
Q36610077Active contraction of microtubule networks
Q30547575Chromosome position at the spindle equator is regulated by chromokinesin and a bipolar microtubule array
Q37832979Do nuclear envelope and intranuclear proteins reorganize during mitosis to form an elastic, hydrogel-like spindle matrix?
Q34059454Functional states of kinetochores revealed by laser microsurgery and fluorescent speckle microscopy
Q38737481High-quality frozen extracts of Xenopus laevis eggs reveal size-dependent control of metaphase spindle micromechanics
Q34630610Insights into the micromechanical properties of the metaphase spindle
Q27309203Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore.
Q38687624Mapping Load-Bearing in the Mammalian Spindle Reveals Local Kinetochore Fiber Anchorage that Provides Mechanical Isolation and Redundancy
Q37621507Mechanical properties of spindle poles are symmetrically balanced
Q37728181Metaphase Spindle Assembly
Q37621924Micromechanics of the vertebrate meiotic spindle examined by stretching along the pole-to-pole axis
Q90455007Microneedle manipulation of the mammalian spindle reveals specialized, short-lived reinforcement near chromosomes
Q33980347Microneedle-based analysis of the micromechanics of the metaphase spindle assembled in Xenopus laevis egg extracts
Q38218123Modelling chromosome dynamics in mitosis: a historical perspective on models of metaphase and anaphase in eukaryotic cells
Q24338855Phase transition of spindle-associated protein regulate spindle apparatus assembly
Q34772202Spindle pole mechanics studied in mitotic asters: dynamic distribution of spindle forces through compliant linkages.
Q35668564The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle.
Q24633928Towards a quantitative understanding of mitotic spindle assembly and mechanics
Q34138127Using micromanipulation to analyze control of vertebrate meiotic spindle size
Q47709213XMAP215 activity sets spindle length by controlling the total mass of spindle microtubules

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