Utility of the clostridial site-specific recombinase TnpX to clone toxic-product-encoding genes and selectively remove genomic DNA fragments

scientific article

Utility of the clostridial site-specific recombinase TnpX to clone toxic-product-encoding genes and selectively remove genomic DNA fragments is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1128/AEM.04285-13
P932PMC publication ID4054144
P698PubMed publication ID24682304
P5875ResearchGate publication ID261220968

P50authorJulian I. RoodQ37375861
Glen P. CarterQ47504414
Dena LyrasQ56437415
Milena AwadQ57053787
Vicki AdamsQ57416190
P2093author name stringJackie K Cheung
Joel Nicholson
Kate E Mackin
Lauren Stevenson
Radhika Bantwal
P2860cites workToxin B is essential for virulence of Clostridium difficileQ24647539
Virulence studies on chromosomal alpha-toxin and theta-toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of alpha-toxin in Clostridium perfringens-mediated gas gangreneQ28301220
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extensionQ29547330
Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinantQ29615039
Insertion sequencesQ29617579
Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integrationQ30527850
Targeted and random bacterial gene disruption using a group II intron (targetron) vector containing a retrotransposition-activated selectable markerQ31134589
Chloramphenicol resistance in Clostridium difficile is encoded on Tn4453 transposons that are closely related to Tn4451 from Clostridium perfringensQ33693870
The VirSR two-component signal transduction system regulates NetB toxin production in Clostridium perfringens.Q33963046
The anti-sigma factor TcdC modulates hypervirulence in an epidemic BI/NAP1/027 clinical isolate of Clostridium difficileQ34055302
Construction of an alpha toxin gene knockout mutant of Clostridium perfringens type A by use of a mobile group II intron.Q34142980
Spo0A differentially regulates toxin production in evolutionarily diverse strains of Clostridium difficileQ35043878
The clostridial mobilisable transposonsQ35060037
Major role for FeoB in Campylobacter jejuni ferrous iron acquisition, gut colonization, and intracellular survivalQ35073883
Regulation of virulence by the RevR response regulator in Clostridium perfringens.Q35075830
The virR gene, a member of a class of two-component response regulators, regulates the production of perfringolysin O, collagenase, and hemagglutinin in Clostridium perfringensQ36105952
Isolation and Characterization of Multiply Antibiotic-Resistant Clostridium perfringens Strains from Porcine FecesQ36470130
FeoA and FeoC are essential components of the Vibrio cholerae ferrous iron uptake system, and FeoC interacts with FeoB.Q37253121
Construction and analysis of chromosomal Clostridium difficile mutantsQ38310436
Development and characterization of a xylose-inducible gene expression system for Clostridium perfringensQ39666377
Identification of Tn4451 and Tn4452, chloramphenicol resistance transposons from Clostridium perfringensQ39956349
Clostridium difficile has two parallel and essential Sec secretion systemsQ41914077
Construction and characterization of a lactose-inducible promoter system for controlled gene expression in Clostridium perfringensQ42068193
ClosTron-mediated engineering of ClostridiumQ42412305
Identification and molecular analysis of a locus that regulates extracellular toxin production in Clostridium perfringensQ42599191
The large resolvase TnpX is the only transposon-encoded protein required for transposition of the Tn4451/3 family of integrative mobilizable elements.Q44792484
DNA binding properties of TnpX indicate that different synapses are formed in the excision and integration of the Tn4451 familyQ45014793
Electroporation-mediated transformation of lysostaphin-treated Clostridium perfringensQ45288245
Transposition of Tn4451 and Tn4453 involves a circular intermediate that forms a promoter for the large resolvase, TnpX.Q47816165
Conjugative transfer of RP4-oriT shuttle vectors from Escherichia coli to Clostridium perfringens.Q48039411
Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivativesQ48221989
Genomic analysis of the erythromycin resistance element Tn5398 from Clostridium difficile.Q48340939
The ClosTron: Mutagenesis in Clostridium refined and streamlined.Q50449586
The peptidoglycan hydrolase TcpG is required for efficient conjugative transfer of pCW3 in Clostridium perfringens.Q54344283
Promoter-probe vectors for the analysis of divergently arranged promoters.Q54437836
Cloning and analysis of the Clostridium perfringens tetracycline resistance plasmid, pCW3.Q54451384
Transferable tetracycline resistance in Clostridium perfringens strains of porcine originQ54490698
Two distinct regions of the large serine recombinase TnpX are required for DNA binding and biological functionQ56917470
Clostridium perfringens-Escherichia coli Shuttle Vectors That Carry Single Antibiotic Resistance DeterminantsQ58029428
The ClosTron: a universal gene knock-out system for the genus ClostridiumQ80687956
P433issue12
P407language of work or nameEnglishQ1860
P304page(s)3597-3603
P577publication date2014-06-01
P1433published inApplied and Environmental MicrobiologyQ4781593
P1476titleUtility of the clostridial site-specific recombinase TnpX to clone toxic-product-encoding genes and selectively remove genomic DNA fragments
P478volume80

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cites work (P2860)
Q58028932Crystal structure of TcpK in complex with oriT DNA of the antibiotic resistance plasmid pCW3
Q41867499Functional analysis of a bacitracin resistance determinant located on ICECp1, a novel Tn916-like element from a conjugative plasmid in Clostridium perfringens
Q35100533Two novel membrane proteins, TcpD and TcpE, are essential for conjugative transfer of pCW3 in Clostridium perfringens

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