Expression of functional influenza virus RNA polymerase in the methylotrophic yeast Pichia pastoris

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Expression of functional influenza virus RNA polymerase in the methylotrophic yeast Pichia pastoris is …
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scholarly articleQ13442814

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P356DOI10.1128/JVI.74.9.4074-4084.2000
P932PMC publication ID111921
P698PubMed publication ID10756019
P5875ResearchGate publication ID12560432

P2093author name stringYamada K
Honda A
Ishihama A
Nakade K
Hwang JS
P2860cites workTwo separate sequences of PB2 subunit constitute the RNA cap-binding site of influenza virus RNA polymeraseQ27863658
RNA polymerase of influenza virus: role of NP in RNA chain elongationQ27865281
In vitro transcription and polymerase binding studies of the termini of influenza A virus cRNA: evidence for a cRNA panhandleQ27865296
Purification and characterization of the SRS2 DNA helicase of the yeast Saccharomyces cerevisiaeQ27935799
Purification, thioredoxin renaturation, and reconstituted activity of the three subunits of the influenza A virus RNA polymeraseQ33659692
Purification and molecular structure of RNA polymerase from influenza virus A/PR8.Q34339090
Expression of the three influenza virus polymerase proteins in a single cell allows growth complementation of viral mutantsQ35600988
Mutational analysis identifies functional domains in the influenza A virus PB2 polymerase subunitQ35856754
Globin mRNAs are primers for the transcription of influenza viral RNA in vitroQ35994818
Promoter analysis of influenza virus RNA polymeraseQ36832112
Identification of a nuclear localization signal of a yeast ribosomal proteinQ37550497
Nucleotides 9 to 11 of the influenza A virion RNA promoter are crucial for activity in vitroQ38323890
Sequence-specific binding of the influenza virus RNA polymerase to sequences located at the 5' ends of the viral RNAs.Q40038550
Recent advances in the expression of foreign genes in Pichia pastorisQ40533559
Review: methylotrophic yeasts as factories for the production of foreign proteinsQ40960629
Recombinant Bordetella pertussis pertactin (P69) from the yeast Pichia pastoris: high-level production and immunological propertiesQ41136918
Molecular assembly of the influenza virus RNA polymerase: determination of the subunit-subunit contact sitesQ41171919
Mutational analysis of influenza virus promoter elements in vivoQ41327528
A multi-functional enzyme with RNA polymerase and RNase activities: molecular anatomy of influenza virus RNA polymeraseQ41470126
Expression of functional influenza virus A polymerase proteins and template from cloned cDNAS in recombinant vaccinia virus infected cellsQ41473829
Application of yeasts in gene expression studies: a comparison of Saccharomyces cerevisiae, Hansenula polymorpha and Kluyveromyces lactis -- a reviewQ41499876
Replication in vitro of the influenza virus genome: selective dissociation of RNA replicase from virus-infected cell ribonucleoprotein complexesQ41500991
The molecular anatomy of influenza virus RNA polymeraseQ41532781
Influenza virus naked RNA can be expressed upon transfection into cells co-expressing the three subunits of the polymerase and the nucleoprotein from simian virus 40 recombinant viruses.Q41571116
An In Vivo Study of the Replication Origin in the Influenza Virus Complementary RNA1Q41588011
Transcription of a recombinant influenza virus RNA in cells that can express the influenza virus RNA polymerase and nucleoprotein genesQ41620677
Reconstitution of influenza virus RNA polymerase from three subunits expressed using recombinant baculovirus systemQ41636089
Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genesQ41668095
Recombinant-baculovirus-expressed PB2 subunit of the influenza A virus RNA polymerase binds cap groups as an isolated subunitQ42064222
High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastorisQ42798376
Enzymatic characterization of purified NS3 serine proteinase of hepatitis C virus expressed in Escherichia coliQ43047406
Synthesis and cellular location of the ten influenza polypeptides individually expressed by recombinant vaccinia virusesQ43756587
In vitro synthesis of influenza viral RNA: biochemical complementation assay of factors required for influenza virus replicationQ44157678
RNA polymerase of influenza virus. III. Isolation of RNA polymerase-RNA complexes from influenza virus PR8.Q45345421
Protection of mice against a lethal influenza virus challenge after immunization with yeast-derived secreted influenza virus hemagglutininQ45750536
Identification of the 5' terminal structure of influenza virus genome RNA by a newly developed enzymatic methodQ45753968
Variation in ATP requirement during influenza virus transcriptionQ45755931
RNA-dependent replication, transcription, and persistence of brome mosaic virus RNA replicons in S. cerevisiaeQ45773637
Mutational analysis of the influenza virus vRNA promoterQ45776505
Synthesis of biologically active influenza virus core proteins using a vaccinia virus-T7 RNA polymerase expression systemQ45780894
Purification and enzymatic properties of an RNA polymerase-RNA complex from influenza virusQ45828953
RNA polymerase of influenza virus. Dinucleotide-primed initiation of transcription at specific positions on viral RNA.Q45831589
Protection of mice against a lethal influenza challenge by immunization with yeast-derived recombinant influenza neuraminidaseQ49093623
High-level secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast, Pichia pastorisQ49167905
Mutations that alter both localization and production of a yeast nuclear proteinQ61631665
Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoterQ63408523
P433issue9
P407language of work or nameEnglishQ1860
P921main subjectPichia pastorisQ148697
P304page(s)4074-4084
P577publication date2000-05-01
P1433published inJournal of VirologyQ1251128
P1476titleExpression of functional influenza virus RNA polymerase in the methylotrophic yeast Pichia pastoris
P478volume74

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cites work (P2860)
Q90419546Alternative Experimental Models for Studying Influenza Proteins, Host-Virus Interactions and Anti-Influenza Drugs
Q45902241Avian influenza A/H5N1 neuraminidase expressed in yeast with a functional head domain.
Q41919256Biochemical characterization of recombinant influenza A polymerase heterotrimer complex: Polymerase activity and mechanisms of action of nucleotide analogs
Q24548325Definition of the minimal viral components required for the initiation of unprimed RNA synthesis by influenza virus RNA polymerase
Q43617488Differential roles of viral RNA and cRNA in functional modulation of the influenza virus RNA polymerase
Q40732397Fine mapping of the subunit binding sites of influenza virus RNA polymerase
Q39669004Influenza A virus polymerase inhibits type I interferon induction by binding to interferon beta promoter stimulator 1.
Q38303101Influenza virus RNA polymerase PA subunit is a novel serine protease with Ser624 at the active site
Q40638142Inhibition of the protease activity of influenza virus RNA polymerase PA subunit by viral matrix protein.
Q34191987Minimum molecular architectures for transcription and replication of the influenza virus
Q39684028The RNA polymerase of influenza a virus is stabilized by interaction with its viral RNA promoter
Q21089768The ambiguous base-pairing and high substrate efficiency of T-705 (Favipiravir) Ribofuranosyl 5'-triphosphate towards influenza A virus polymerase