A two-step path to inclusion formation of huntingtin peptides revealed by number and brightness analysis

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A two-step path to inclusion formation of huntingtin peptides revealed by number and brightness analysis is …
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scholarly articleQ13442814

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P356DOI10.1016/J.BPJ.2010.02.058
P932PMC publication ID2884247
P698PubMed publication ID20550921
P5875ResearchGate publication ID44675941

P50authorEnrico GrattonQ62059124
Michelle A DigmanQ62062552
P2093author name stringJ Lawrence Marsh
Giulia Ossato
Charity Aiken
Tamas Lukacsovich
P2860cites workSUMO modification of Huntingtin and Huntington's disease pathologyQ24324137
Huntingtin aggregation and toxicity in Huntington's diseaseQ28204163
Protein folding and misfoldingQ28235199
Formation of neuronal intranuclear inclusions underlies the neurological dysfunction in mice transgenic for the HD mutationQ28246858
Huntington's diseaseQ28284355
Inclusion body formation reduces levels of mutant huntingtin and the risk of neuronal deathQ28287762
Common structure of soluble amyloid oligomers implies common mechanism of pathogenesisQ29547501
Inherent toxicity of aggregates implies a common mechanism for protein misfolding diseasesQ29616535
Histone deacetylase inhibitors arrest polyglutamine-dependent neurodegeneration in DrosophilaQ29616737
Aggregation of huntingtin in neuronal intranuclear inclusions and dystrophic neurites in brainQ29617982
Mapping the number of molecules and brightness in the laser scanning microscopeQ30441376
Determination of particle number and brightness using a laser scanning confocal microscope operating in the analog modeQ30444740
Caspase 3-cleaved N-terminal fragments of wild-type and mutant huntingtin are present in normal and Huntington's disease brains, associate with membranes, and undergo calpain-dependent proteolysis.Q33948101
Expanded polyglutamines in Caenorhabditis elegans cause axonal abnormalities and severe dysfunction of PLM mechanosensory neurons without cell deathQ33949349
Huntington's disease age-of-onset linked to polyglutamine aggregation nucleationQ34154622
The photon counting histogram in fluorescence fluctuation spectroscopyQ34170903
Protein misfolding, amyloid formation, and neurodegeneration: a critical role for molecular chaperones?Q34747079
Role of proteolysis in polyglutamine disordersQ35575850
Kinetics and thermodynamics of amyloid fibril assemblyQ36596626
Distinct conformations of in vitro and in vivo amyloids of huntingtin-exon1 show different cytotoxicityQ37209664
Quantitative comparison of different fluorescent protein couples for fast FRET-FLIM acquisition.Q37392567
Aggresomes protect cells by enhancing the degradation of toxic polyglutamine-containing proteinQ40662036
Expanded polyglutamine peptides alone are intrinsically cytotoxic and cause neurodegeneration in DrosophilaQ41705884
Fibrils formed in vitro from alpha-synuclein and two mutant forms linked to Parkinson's disease are typical amyloidQ42620617
Generation and characterization of embryonic striatal conditionally immortalized ST14A cellsQ42818500
Polyglutamine disruption of the huntingtin exon 1 N terminus triggers a complex aggregation mechanismQ43100835
Aggregated polyglutamine peptides delivered to nuclei are toxic to mammalian cellsQ44189907
Pivotal role of oligomerization in expanded polyglutamine neurodegenerative disorders.Q44286601
Polyglutamine expansion and Huntington's diseaseQ45296525
Decreased expression of hypothalamic neuropeptides in Huntington disease transgenic mice with expanded polyglutamine-EGFP fluorescent aggregates.Q45296599
Nuclear and neuropil aggregates in Huntington's disease: relationship to neuropathology.Q45297497
Detection of polyglutamine protein oligomers in cells by fluorescence correlation spectroscopyQ45305431
Reconsidering the mechanism of polyglutamine peptide aggregationQ45306646
Time-lapse analysis of aggregate formation in an inducible PC12 cell model of Huntington's disease reveals time-dependent aggregate formation that transiently delays cell death.Q45307351
A Drosophila ortholog of the human MRJ modulates polyglutamine toxicity and aggregationQ47072548
Huntingtin acts in the nucleus to induce apoptosis but death does not correlate with the formation of intranuclear inclusions.Q48373564
Ectopically Expressed CAG Repeats Cause Intranuclear Inclusions and a Progressive Late Onset Neurological Phenotype in the MouseQ48574817
The mechanism of islet amyloid polypeptide toxicity is membrane disruption by intermediate-sized toxic amyloid particles.Q52534249
Amyloid formation under physiological conditions proceeds via a native-like folding intermediateQ52856652
Mutation of the E6-AP ubiquitin ligase reduces nuclear inclusion frequency while accelerating polyglutamine-induced pathology in SCA1 miceQ54066632
Expression of an expanded polyglutamine domain in yeast causes death with apoptotic markersQ79753117
P433issue12
P407language of work or nameEnglishQ1860
P1104number of pages8
P304page(s)3078-3085
P577publication date2010-06-01
P1433published inBiophysical JournalQ2032955
P1476titleA two-step path to inclusion formation of huntingtin peptides revealed by number and brightness analysis
P478volume98

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cites work (P2860)
Q54960388A Liquid to Solid Phase Transition Underlying Pathological Huntingtin Exon1 Aggregation.
Q59799803A discontinuous Galerkin model for fluorescence loss in photobleaching of intracellular polyglutamine protein aggregates
Q38974551A link between chromatin condensation mechanisms and Huntington's disease: connecting the dots.
Q41513084Aggregation behavior of chemically synthesized, full-length huntingtin exon1.
Q38038981An in vitro perspective on the molecular mechanisms underlying mutant huntingtin protein toxicity
Q30660872Application limits and data correction in number of molecules and brightness analysis
Q35464104Application of three-photon excitation FCS to the study of protein oligomerization
Q36168138Arbitrary-Region Raster Image Correlation Spectroscopy.
Q91584069Assaying Homodimers of NF-κB in Live Single Cells
Q34181169Assessing the contribution of heterogeneous distributions of oligomers to aggregation mechanisms of polyglutamine peptides
Q39093738Backbone Engineering within a Latent β-Hairpin Structure to Design Inhibitors of Polyglutamine Amyloid Formation
Q33675599Biophysical underpinnings of the repeat length dependence of polyglutamine amyloid formation
Q38777301Cellular models for Parkinson's disease.
Q27301252Comparative study of naturally occurring huntingtin fragments in Drosophila points to exon 1 as the most pathogenic species in Huntington's disease
Q36250960Conformational dynamics and self-association of intrinsically disordered Huntingtin exon 1 in cells
Q30724979Delayed emergence of subdiffraction-sized mutant huntingtin fibrils following inclusion body formation
Q35239346Disruption of the nuclear membrane by perinuclear inclusions of mutant huntingtin causes cell-cycle re-entry and striatal cell death in mouse and cell models of Huntington's disease.
Q33663833Distinct cellular toxicity of two mutant huntingtin mRNA variants due to translation regulation.
Q30523072Dynamic Imaging by Fluorescence Correlation Spectroscopy Identifies Diverse Populations of Polyglutamine Oligomers Formed in Vivo
Q36016804Dynamics and Distribution of Klothoβ (KLB) and Fibroblast Growth Factor Receptor-1 (FGFR1) in Living Cells Reveal the Fibroblast Growth Factor-21 (FGF21)-induced Receptor Complex
Q35597773Expression-level dependent perturbation of cell proteostasis and nuclear morphology by aggregation-prone polyglutamine proteins.
Q34536409Fluctuation methods to study protein aggregation in live cells: concanavalin A oligomers formation
Q36043574Folding Landscape of Mutant Huntingtin Exon1: Diffusible Multimers, Oligomers and Fibrils, and No Detectable Monomer
Q30994681Formation and toxicity of soluble polyglutamine oligomers in living cells
Q99233981GFP fluorescence tagging alters dynamin-related protein 1 oligomerization dynamics and creates disassembly-refractory puncta to mediate mitochondrial fission
Q34328850Huntingtin Fragments and SOD1 Mutants Form Soluble Oligomers in the Cell
Q56083219Huntington disease
Q37889820Huntington's disease, calcium, and mitochondria
Q35801979Intact microtubules preserve transient receptor potential vanilloid 1 (TRPV1) functionality through receptor binding
Q33922305Investigating the structural impact of the glutamine repeat in huntingtin assembly
Q36620955Lessons in fluctuation correlation spectroscopy
Q35016698Ligand-induced dynamics of neurotrophin receptors investigated by single-molecule imaging approaches
Q35650935Live Cell Characterization of DNA Aggregation Delivered through Lipofection
Q35965461Live-cell observation of cytosolic HIV-1 assembly onset reveals RNA-interacting Gag oligomers
Q28655103Measuring protein dynamics in live cells: protocols and practical considerations for fluorescence fluctuation microscopy
Q98181137Nascent mutant Huntingtin exon 1 chains do not stall on ribosomes during translation but aggregates do recruit machinery involved in ribosome quality control and RNA
Q39021697Number and Brightness analysis of alpha-synuclein oligomerization and the associated mitochondrial morphology alterations in live cells
Q35731664Number and brightness analysis of sFRP4 domains in live cells demonstrates vesicle association signal of the NLD domain and dynamic intracellular responses to Wnt3a
Q30503536Number and brightness image analysis reveals ATF-induced dimerization kinetics of uPAR in the cell membrane
Q38107677Pharmacological protein targets in polyglutamine diseases: Mutant polypeptides and their interactors
Q37981477Physical Chemistry of Polyglutamine: Intriguing Tales of a Monotonous Sequence
Q39124557Proteins Containing Expanded Polyglutamine Tracts and Neurodegenerative Disease
Q38178152Quantifying intracellular dynamics using fluorescence fluctuation spectroscopy
Q21284320Quantitative fluorescence loss in photobleaching for analysis of protein transport and aggregation.
Q35020201Scanning fluorescence correlation spectroscopy techniques to quantify the kinetics of DNA double strand break repair proteins after γ-irradiation and bleomycin treatment
Q26860528Scanning image correlation spectroscopy
Q46324287Sedimentation Velocity Analysis with Fluorescence Detection of Mutant Huntingtin Exon 1 Aggregation in Drosophila melanogaster and Caenorhabditis elegans.
Q36600026Slow amyloid nucleation via α-helix-rich oligomeric intermediates in short polyglutamine-containing huntingtin fragments.
Q48138939Spatiotemporal Analysis of K-Ras Plasma Membrane Interactions Reveals Multiple High Order Homo-oligomeric Complexes.
Q55287168State-of-the-Art Fluorescence Fluctuation-Based Spectroscopic Techniques for the Study of Protein Aggregation.
Q34978135Structural formation of huntingtin exon 1 aggregates probed by small-angle neutron scattering.
Q38165760Studying neurodegenerative diseases in culture models.
Q36749672Studying polyglutamine aggregation in Caenorhabditis elegans using an analytical ultracentrifuge equipped with fluorescence detection.
Q35810050Supramolecular Non-Amyloid Intermediates in the Early Stages of α-Synuclein Aggregation
Q39377946Tracking protein aggregation and mislocalization in cells with flow cytometry
Q52344126Two-detector number and brightness analysis reveals spatio-temporal oligomerization of proteins in living cells.
Q30530785Two-photon time-lapse microscopy of BODIPY-cholesterol reveals anomalous sterol diffusion in chinese hamster ovary cells
Q42130355Unveiling LOX-1 receptor interplay with nanotopography: mechanotransduction and atherosclerosis onset.
Q63359409Using enhanced number and brightness to measure protein oligomerization dynamics in live cells

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