Attainment of 15-fold higher affinity of a Fusarium-specific single-chain antibody by directed molecular evolution coupled to phage display

scientific article

Attainment of 15-fold higher affinity of a Fusarium-specific single-chain antibody by directed molecular evolution coupled to phage display is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1007/S12033-011-9478-3
P698PubMed publication ID22161226
P5875ResearchGate publication ID51870921

P2093author name stringHe-Ping Li
Jing-Bo Zhang
Yu-Cai Liao
Shu Xing
Sheng Xue
Jin-Long Liu
Zu-Quan Hu
P2860cites workSingle-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistanceQ45627435
Engineering Fusarium head blight resistance in wheat by expression of a fusion protein containing a Fusarium-specific antibody and an antifungal peptideQ46431608
Plant pathology. Deadly wheat fungus threatens world's breadbasketsQ46544045
Coordinate expression of antibody subunit genes yields high levels of functional antibodies in roots of transgenic tobaccoQ48077488
Determination of the trichothecene mycotoxin chemotypes and associated geographical distribution and phylogenetic species of the Fusarium graminearum clade from China.Q51105018
Determination of rate and equilibrium binding constants for macromolecular interactions using surface plasmon resonance: use of nonlinear least squares analysis methods.Q52392958
The effect of high-frequency random mutagenesis on in vitro protein evolution: a study on TEM-1 beta-lactamaseQ56902347
Direct random mutagenesis of gene-sized DNA fragments using polymerase chain reactionQ72141004
The cell wall of Fusarium oxysporumQ78109516
Functional improvement of antibody fragments using a novel phage coat protein III fusion systemQ78454268
Economic and social impacts of fusarium head blight: changing farms and rural communities in the northern great plainsQ79685796
Expression of anti-sclerotinia scFv in transgenic Brassica napus enhances tolerance against stem rotQ85174018
DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolutionQ24562755
In vitro evolution of single-chain antibodies using mRNA displayQ24672839
Directed in vitro evolution and crystallographic analysis of a peptide-binding single chain antibody fragment (scFv) with low picomolar affinityQ27643060
Phage display: practicalities and prospectsQ28202044
Rapid evolution of a protein in vitro by DNA shufflingQ28245873
Selection of phage antibodies to surface epitopes of Phytophthora infestans.Q30810596
Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodiesQ30841593
Directed evolution of an anti-carcinoembryonic antigen scFv with a 4-day monovalent dissociation half-time at 37 degrees C.Q31054095
Fusion proteins comprising a Fusarium-specific antibody linked to antifungal peptides protect plants against a fungal pathogenQ31061707
Affinity maturation of Fab antibody fragments by fluorescent-activated cell sorting of yeast-displayed librariesQ31148151
Engineering the steroid-specificity of an anti-17beta-estradiol Fab by random mutagenesis and competitive phage panningQ32062476
Directed evolution, phage display and combination of evolved mutants: a strategy to recover the neutralization properties of the scFv version of BCF2 a neutralizing monoclonal antibody specific to scorpion toxin Cn2.Q33211955
A strategy for the generation of specific human antibodies by directed evolution and phage display. An example of a single-chain antibody fragment that neutralizes a major component of scorpion venomQ33214905
Directed evolution of an anti-prion protein scFv fragment to an affinity of 1 pM and its structural interpretationQ33256919
Recombinant-antibody-mediated resistance against Tomato yellow leaf curl virus in Nicotiana benthamianaQ33411320
Two-step in vitro antibody affinity maturation enables estradiol-17beta assays with more than 10-fold higher sensitivityQ33521742
Antibody-based resistance to plant pathogensQ34185211
Novel human DNA alkyltransferases obtained by random substitution and genetic selection in bacteriaQ36801155
Grapevine fanleaf virus (GFLV)-specific antibodies confer GFLV and Arabis mosaic virus (ArMV) resistance in Nicotiana benthamiana.Q38357206
Exploring the functional robustness of an enzyme by in vitro evolutionQ41064129
Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attackQ44122920
Stable chelating linkage for reversible immobilization of oligohistidine tagged proteins in the BIAcore surface plasmon resonance detectorQ45155222
Transgenic expression in citrus of single-chain antibody fragments specific to Citrus tristeza virus confers virus resistanceQ45378239
Plantibody-mediated inhibition of the Potato leafroll virus P1 protein reduces virus accumulation.Q45394216
P433issue2
P921main subjectphage displayQ896217
P304page(s)111-122
P577publication date2012-10-01
P1433published inMolecular BiotechnologyQ15761195
P1476titleAttainment of 15-fold higher affinity of a Fusarium-specific single-chain antibody by directed molecular evolution coupled to phage display
P478volume52

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cites work (P2860)
Q46616336An antibody that confers plant disease resistance targets a membrane-bound glyoxal oxidase in Fusarium
Q47425510Antibody Affinity and Stability Maturation by Error-Prone PCR.
Q93068254Antibody discovery and engineering by enhanced CRISPR-Cas9 integration of variable gene cassette libraries in mammalian cells
Q35639609Characterization of single-domain antibodies against Foot and Mouth Disease Virus (FMDV) serotype O from a camelid and imaging of FMDV in baby hamster kidney-21 cells with single-domain antibody-quantum dots probes
Q34353750Generation of a highly reactive chicken-derived single-chain variable fragment against Fusarium verticillioides by phage display
Q35148842Selection and identification of single-domain antibody fragment against capsid protein of porcine circovirus type 2 (PCV2) from C. bactrianus

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