Site-directed fluorescence labeling reveals a revised N-terminal membrane topology and functional periplasmic residues in the Escherichia coli cell division protein FtsK

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Site-directed fluorescence labeling reveals a revised N-terminal membrane topology and functional periplasmic residues in the Escherichia coli cell division protein FtsK is …
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scholarly articleQ13442814

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P356DOI10.1074/JBC.M114.569624
P932PMC publication ID4156081
P698PubMed publication ID25002583

P2093author name stringAlison M Berezuk
Cezar M Khursigara
Mara Goodyear
P2860cites work3D-SIM super resolution microscopy reveals a bead-like arrangement for FtsZ and the division machinery: implications for triggering cytokinesisQ21145732
Condensation of FtsZ filaments can drive bacterial cell divisionQ24646706
Reconstitution of contractile FtsZ rings in liposomesQ24650388
Asymmetry of chromosome Replichores renders the DNA translocase activity of FtsK essential for cell division and cell shape maintenance in Escherichia coliQ27313431
Structural and mutational analysis of the cell division protein FtsQQ27649970
Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide GelsQ27860531
The HMMTOP transmembrane topology prediction serverQ28190632
Conformational parameters for amino acids in helical, beta-sheet, and random coil regions calculated from proteinsQ28240446
Prediction of protein conformationQ28240456
FtsZ ring structure associated with division in Escherichia coliQ28245159
Principles governing amino acid composition of integral membrane proteins: application to topology predictionQ28285162
FtsK Is a DNA motor protein that activates chromosome dimer resolution by switching the catalytic state of the XerC and XerD recombinasesQ28427479
Three functional subdomains of the Escherichia coli FtsQ protein are involved in its interaction with the other division proteinsQ28485214
FtsZ in bacterial cytokinesis: cytoskeleton and force generator all in one.Q30497729
Cytokinesis in bacteriaQ33186319
From the regulation of peptidoglycan synthesis to bacterial growth and morphology.Q33694168
Fully efficient chromosome dimer resolution in Escherichia coli cells lacking the integral membrane domain of FtsK.Q33697032
Localization of cell division protein FtsK to the Escherichia coli septum and identification of a potential N-terminal targeting domainQ33726106
Only the N-terminal domain of FtsK functions in cell divisionQ33736636
Role of the C terminus of FtsK in Escherichia coli chromosome segregationQ33743902
Transmembrane protein topology mapping by the substituted cysteine accessibility method (SCAM(TM)): application to lipid-specific membrane protein topogenesisQ33922207
Membrane-associated DNA transport machinesQ33931986
Topology of OxlT, the oxalate transporter of Oxalobacter formigenes, determined by site-directed fluorescence labelingQ33995996
Distinct penicillin binding proteins involved in the division, elongation, and shape of Escherichia coli K12Q35085145
A new Escherichia coli cell division gene, ftsKQ35597632
Inactivation of FtsI inhibits constriction of the FtsZ cytokinetic ring and delays the assembly of FtsZ rings at potential division sitesQ35954752
Escherichia coli cell division protein FtsZ is a guanine nucleotide binding proteinQ36087722
Diverse paths to midcell: assembly of the bacterial cell division machineryQ36187570
ZipA is required for FtsZ-dependent preseptal peptidoglycan synthesis prior to invagination during cell division.Q36276314
Evidence for involvement of penicillin-binding protein 3 in murein synthesis during septation but not during cell elongationQ36323172
Cell division and peptidoglycan assembly in Escherichia coliQ36405514
Evidence for functional overlap among multiple bacterial cell division proteins: compensating for the loss of FtsK.Q36592350
In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acidsQ36660084
FtsK, a literate chromosome segregation machineQ36826102
Force generation by a dynamic Z-ring in Escherichia coli cell divisionQ37068245
TheEscherichia colicell cycle: one cycle or multiple independent processes that are co-ordinated?Q37197628
The N-terminal membrane-spanning domain of the Escherichia coli DNA translocase FtsK hexamerizes at midcellQ37408449
Synchronization of chromosome dynamics and cell division in bacteriaQ37698253
Bacterial cytokinesis: From Z ring to divisomeQ38034197
The FtsK Family of DNA Pumps.Q38060580
KOPS: DNA motifs that control E. coli chromosome segregation by orienting the FtsK translocaseQ39491027
FtsZ dynamics during the division cycle of live Escherichia coli cellsQ39565511
New insights into the developmental history of the bacterial cell division siteQ39706148
Effects of multiple deletions of murein hydrolases on viability, septum cleavage, and sensitivity to large toxic molecules in Escherichia coliQ39741336
A mutation in the ftsK gene of Escherichia coli affects cell-cell separation, stationary-phase survival, stress adaptation, and expression of the gene encoding the stress protein UspA.Q39847240
FtsL, an essential cytoplasmic membrane protein involved in cell division in Escherichia coliQ39939982
Rate and topography of peptidoglycan synthesis during cell division in Escherichia coli: concept of a leading edgeQ39949309
FtsZ ring formation in fts mutantsQ40024436
Identification of new genes in a cell envelope-cell division gene cluster of Escherichia coli: cell division gene ftsQ.Q40338122
FtsZ, a prokaryotic homolog of tubulin?Q40595303
Functional and structural characterization of polysaccharide co-polymerase proteins required for polymer export in ATP-binding cassette transporter-dependent capsule biosynthesis pathwaysQ42098473
Dancing around the divisome: asymmetric chromosome segregation in Escherichia coliQ42548452
Creation of a Fully Functional Cysteine-Less Variant of Osmosensor and Proton-Osmoprotectant Symporter ProP from Escherichia coli and Its Application to Assess the Transporter's Membrane OrientationQ44608212
A complex of the Escherichia coli cell division proteins FtsL, FtsB and FtsQ forms independently of its localization to the septal regionQ44912239
Cell cycle-dependent duplication and bidirectional migration of SeqA-associated DNA-protein complexes in E. coliQ47858624
The cytoplasmic domain of FtsK protein is required for resolution of chromosome dimers.Q52179251
FtsK--a bacterial cell division checkpoint?Q53283360
Multiple regions along the Escherichia coli FtsK protein are implicated in cell divisionQ53288341
The Escherichia coli FtsK functional domains involved in its interaction with its divisome protein partners.Q53457170
FtsK and SpoIIIE: the tale of the conserved tails.Q54431845
Use of a two-hybrid assay to study the assembly of a complex multicomponent protein machinery: bacterial septosome differentiation.Q54514229
Double-Stranded DNA Translocation: Structure and Mechanism of Hexameric FtsKQ57910320
Bacillus subtilis spoIIIE protein required for DNA segregation during asymmetric cell divisionQ57990639
Membrane topology of the N-terminus of the Escherichia coli FtsK division proteinQ74099086
FtsQ, FtsL and FtsI require FtsK, but not FtsN, for co-localization with FtsZ during Escherichia coli cell divisionQ77155405
FtsK is an essential cell division protein that is localized to the septum and induced as part of the SOS responseQ77179856
FtsK is a bifunctional protein involved in cell division and chromosome localization in Escherichia coliQ77179898
A structural model for the osmosensor, transporter, and osmoregulator ProP of Escherichia coliQ81637305
P4510describes a project that usesImageJQ1659584
P433issue34
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
fluorescence labellingQ124308791
P304page(s)23287-23301
P577publication date2014-07-07
P1433published inJournal of Biological ChemistryQ867727
P1476titleSite-directed fluorescence labeling reveals a revised N-terminal membrane topology and functional periplasmic residues in the Escherichia coli cell division protein FtsK
P478volume289

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cites work (P2860)
Q28364545Cell age dependent concentration of Escherichia coli divisome proteins analyzed with ImageJ and ObjectJ
Q45931672Comparative Analyses of Transport Proteins Encoded within the Genomes of Bdellovibrio bacteriovorus HD100 and Bdellovibrio exovorus JSS.
Q39429979How bacterial cell division might cheat turgor pressure - a unified mechanism of septal division in Gram-positive and Gram-negative bacteria
Q52723279Imaging Bacterial Cell Wall Biosynthesis.
Q50551122Mapping of Membrane Protein Topology by Substituted Cysteine Accessibility Method (SCAM™).
Q58699719Outer membrane lipoprotein RlpA is a novel periplasmic interaction partner of the cell division protein FtsK in Escherichia coli
Q28829075Structure and Mutational Analyses of Escherichia coli ZapD Reveal Charged Residues Involved in FtsZ Filament Bundling
Q49290972Turgor Pressure and Possible Constriction Mechanisms in Bacterial Division
Q39217854Xer Site Specific Recombination: Double and Single Recombinase Systems

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