Improving enzymes by using them in organic solvents

scientific article

Improving enzymes by using them in organic solvents is …
instance of (P31):
review articleQ7318358
scholarly articleQ13442814

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P6179Dimensions Publication ID1007017175
P356DOI10.1038/35051719
P698PubMed publication ID11196652

P2093author name stringKlibanov AM
P2860cites workEnzyme-catalyzed processes in organic solventsQ24567816
Crystal structure of triosephosphate isomerase from Trypanosoma cruzi in hexaneQ27619540
X-ray crystal structure of gamma-chymotrypsin in hexaneQ27730806
Enzyme crystal structure in a neat organic solventQ27732014
The crystal structure of subtilisin Carlsberg in anhydrous dioxane and its comparison with those in water and acetonitrileQ27736243
X-ray studies on two forms of bovine beta-trypsin crystals in neat cyclohexaneQ27766741
Solid-state nuclear magnetic resonance investigation of solvent dependence of tyrosyl ring motion in an enzymeQ31161921
Enzymatic synthesis and modification of polymers in nonaqueous solventsQ33543838
Lyophilization-induced reversible changes in the secondary structure of proteinsQ33746835
Enzymatic catalysis in anhydrous organic solventsQ34502157
Striking activation of oxidative enzymes suspended in nonaqueous mediaQ36421089
Hydration of Proteins and PolypeptidesQ36521540
Solvent dielectric effects on protein dynamicsQ37047257
Preparation of deglycosylated egg white avidinQ38297151
The solvent dependence of enzyme specificityQ40753893
Why are enzymes less active in organic solvents than in water?Q41396033
Kinetics and specificity of serine proteases in peptide synthesis catalyzed in organic solventsQ42195682
Can conformational changes be responsible for solvent and excipient effects on the catalytic behavior of subtilisin Carlsberg in organic solvents?Q47862187
Remarkable activation of enzymes in nonaqueous media by denaturing organic cosolventsQ47864442
Enzyme thermoinactivation in anhydrous organic solventsQ47869092
Enzyme memory. What is remembered and why?Q52053463
Trapping of different lipase conformers in water-restricted environments.Q53984629
How do organic solvents affect peroxidase structure and function?Q67486653
Enzyme catalysis in organic solvents with low water content at high temperatures. The adenosinetriphosphatase of submitochondrial particlesQ68417286
A colorimetric method for the enzymatic analysis of gases: the determination of ethanol and formaldehyde vapors using solid alcohol oxidaseQ69562134
Enzymatic catalysis in organic media at 100 degrees CQ71372467
'Organic phase buffers' control biocatalyst activity independent of initial aqueous pHQ71994649
Enzymic catalysis in heterogeneous eutectic mixtures of substratesQ72273140
On enzymatic activity in organic solvents as a function of enzyme historyQ74659909
Enzymatic resolution of racemic amines in a continuous reactor in organic solventsQ81580248
Dramatic enhancement of enzymatic activity in organic solvents by lyoprotectantsQ81600948
Transition state stabilization of subtilisins in organic mediaQ81614198
P433issue6817
P407language of work or nameEnglishQ1860
P1104number of pages6
P304page(s)241-246
P577publication date2001-01-01
P1433published inNatureQ180445
P1476titleImproving enzymes by using them in organic solvents
P478volume409

Reverse relations

cites work (P2860)
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Q40764785Characterization and Crystal Structure of a Robust Cyclohexanone Monooxygenase.
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Q34836756Characterization of a cold-adapted and salt-tolerant esterase from a psychrotrophic bacterium Psychrobacter pacificensis
Q41883456Characterization of a novel lipase from Bacillus sp. isolated from tannery wastes
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Q44644900Characterization of a thermally stable and organic solvent-adaptative NAD+ -dependent formate dehydrogenase from Bacillus sp. F1.
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Q111659069Chemoenzymatic Synthesis of Original Stilbene Dimers Possessing Wnt Inhibition Activity in Triple-Negative Breast Cancer Cells Using the Enzymatic Secretome of Botrytis cinerea Pers.
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Q40112601Circular dichroism and UV resonance raman study of the impact of alcohols on the Gibbs free energy landscape of an alpha-helical peptide
Q39804873Cloning of a nitrilase gene from the cyanobacterium Synechocystis sp. strain PCC6803 and heterologous expression and characterization of the encoded protein
Q53807830Cloning, expression, purification and characterization of lipase from Bacillus licheniformis, isolated from hot spring of Himachal Pradesh, India.
Q58208866Co-oxidation of β-carotene in biphasic media
Q42423731Combination of oxyanion Gln114 mutation and medium engineering to influence the enantioselectivity of thermophilic lipase from Geobacillus zalihae
Q56950651Combining regio- and enantioselectivity of lipases for the preparation of (R)-4-chloro-2-butanol
Q39044080Comparison of lipases and glycoside hydrolases as catalysts in synthesis reactions
Q33188500Comparison of theoretical and experimental data to evaluate substrate diffusional limitations for crown ether- and methyl-beta-cyclodextrin-activated serine protease subtilisin Carlsberg in tetrahydrofuran
Q56948565Computational design of a lipase for catalysis of the Diels-Alder reaction
Q44124236Computer-aided real-time estimation of reaction conversion for lipase-catalyzed esterification in solvent-free systems
Q83948842Conditioning action of the environment on the protein dynamics studied through elastic neutron scattering
Q54381194Conformation and catalytic properties studies of Candida rugosa Lip7 via enantioselective esterification of ibuprofen in organic solvents and ionic liquids.
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Q43027755Construction of an Immobilized Thermophilic Esterase on Epoxy Support for Poly(ε-caprolactone) Synthesis
Q35100838Construction of the yeast whole-cell Rhizopus oryzae lipase biocatalyst with high activity
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Q97094561DMSO tolerant NAD(P)H recycler enzyme from a pathogenic bacterium, Burkholderia dolosa PC543: effect of N-/C-terminal His Tag extension on protein solubility and activity
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Q57258646Droplet microfluidics: A tool for biology, chemistry and nanotechnology
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Q50267723Effect of Linker Length and Flexibility on the Clostridium thermocellum Esterase Displayed on Bacillus subtilis Spores
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Q43017061Effect of organic solvents on the activity and stability of halophilic alcohol dehydrogenase (ADH2) from Haloferax volcanii
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Q30432286Effects of organic solvents and substrate binding on trypsin in acetonitrile and hexane media
Q44186056Effects of water content on the tetrahedral intermediate of chymotrypsin - trifluoromethylketone in polar and non-polar media: observations from molecular dynamics simulation
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Q56950758Efficient kinetic resolution of (R,S)-1-trimethylsilylethanol via lipase-mediated enantioselective acylation in ionic liquids
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Q46333162Enzymatic Activation of the Emerging Drug Resveratrol
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Q42168966Enzymatic regioselective levulinylation of 2'-deoxyribonucleosides and 2'-o-methylribonucleosides
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Q34441915Enzyme activity in liquid lipase melts as a step towards solvent-free biology at 150 °C.
Q35025990Enzyme catalysis in ionic liquids
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Q64897286Evaluation of the Candida sp. 99-125 Lipase Positional Selectivity for 1,3-Diolein Synthesis.
Q37974847Expanding the organic toolbox: a guide to integrating biocatalysis in synthesis
Q27684377Expansion of access tunnels and active-site cavities influence activity of haloalkane dehalogenases in organic cosolvents
Q91344779Exploration of anti-insect potential of trypsin inhibitor purified from seeds of Sapindus mukorossi against Bactrocera cucurbitae
Q48137379Exploration of the expeditious potential of Pseudomonas fluorescens lipase in the kinetic resolution of racemic intermediates and its validation through molecular docking.
Q52985718Exploring mild enzymatic sustainable routes for the synthesis of bio-degradable aromatic-aliphatic oligoesters.
Q55262871Expression and characterization of a 9-cis-epoxycarotenoid dioxygenase from Serratia sp. ATCC 39006 capable of biotransforming isoeugenol and 4-vinylguaiacol to vanillin.
Q43025487Expression and display of a novel thermostable esterase from Clostridium thermocellum on the surface of Bacillus subtilis using the CotB anchor protein
Q34904182Extreme halophilic enzymes in organic solvents
Q35165269Extremophiles as a source for novel enzymes
Q33638911Factors that influence helical preferences for singly charged gas-phase peptide ions: the effects of multiple potential charge-carrying sites
Q89509524Fine Modulation of the Catalytic Properties of Rhizomucor miehei Lipase Driven by Different Immobilization Strategies for the Selective Hydrolysis of Fish Oil
Q44589997Fluorescent organophosphonates as inhibitors of microbial lipases
Q33305799Fragrance release from the surface of branched poly (amide)s.
Q34174518Gene cloning and characterization of a novel highly organic solvent tolerant lipase from Proteus sp. SW1 and its application for biodiesel production.
Q97684623Generation of Stilbene Antimicrobials against Multiresistant Strains of Staphylococcus aureus through Biotransformation by the Enzymatic Secretome of Botrytis cinerea
Q30777925Generation of a broad esterolytic subtilisin using combined molecular evolution and periplasmic expression
Q43022176Glutaraldehyde cross-linking of immobilized thermophilic esterase on hydrophobic macroporous resin for application in poly(ε-caprolactone) synthesis.
Q56927777Glycerol carbonate: a novel biosolvent with strong ionizing and dissociating powers
Q89069959Glycyrrhetinic Acid Liposomes Containing Mannose-Diester Lauric Diacid-Cholesterol Conjugate Synthesized by Lipase-Catalytic Acylation for Liver-Specific Delivery
Q64935339Graphene oxide enabled long-term enzymatic transesterification in an anhydrous gas flux.
Q24298741Guide molecule-driven stereospecific degradation of alpha-methylpolyamines by polyamine oxidase
Q43032413Haloarchaea: worth exploring for their biotechnological potential
Q48295847Hemin-micelles immobilized in alginate hydrogels as artificial enzymes with peroxidase-like activity and substrate selectivity.
Q58306575Hemoglobin Modified Carbon Paste Electrode: Direct Electrochemistry and Electrocatalysis
Q45802898Heterologous expression and biochemical characterization of acetyl xylan esterase from Coprinopsis cinerea
Q43018284Heterologous overexpression, purification and characterisation of an alcohol dehydrogenase (ADH2) from Halobacterium sp. NRC-1.
Q58587291High-level expression and molecular characterization of a recombinant prolidase from NovaBlue
Q34021716High-resolution NMR spectroscopy of encapsulated proteins dissolved in low-viscosity fluids
Q36976667Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method
Q48280177Highly Productive and Enantioselective Enzyme Catalysis under Continuous Supported Liquid-Liquid Conditions Using a Hybrid Monolithic Bioreactor
Q35348425Highly efficient and enzymatic regioselective undecylenoylation of gastrodin in 2-methyltetrahydrofuran-containing systems
Q44359777Highly sensitive active-site titration of lipase in microscale culture media using fluorescent organophosphorus ester
Q56336048How Does Solvation Layer Mobility Affect Protein Structural Dynamics?
Q34186736Hydration of enzyme in nonaqueous media is consistent with solvent dependence of its activity
Q33767676Hydration potential of lysozyme: protein dehydration using a single microparticle technique
Q33382788Hydrogen/deuterium exchange study of subtilisin Carlsberg during prolonged exposure to organic solvents
Q28468553Identification and Molecular Characterization of Genes Coding Pharmaceutically Important Enzymes from Halo-Thermo Tolerant Bacillus
Q54342446Identification and reconstitution of genetic regulatory networks for improved microbial tolerance to isooctane.
Q36918325Immobilization and Biochemical Properties of the Enantioselective Recombinant NStcI Esterase of Aspergillus nidulans.
Q35566066Immobilization and characterization of a new regioselective and enantioselective lipase obtained from a metagenomic library
Q42848494Immobilization of a Commercial Lipase from Penicillium camembertii (Lipase G) by Different Strategies.
Q34298757Immobilization of lipase B within micron-sized poly-N-isopropylacrylamide hydrogel particles by solvent exchange
Q44935173Immobilization of lipases on different carriers and their use in synthesis of pentyl isovalerates
Q35749453Immobilization of trypsin in organic and aqueous media for enzymatic peptide synthesis and hydrolysis reactions
Q35720111Immobilization, Regiospecificity Characterization and Application of Aspergillus oryzae Lipase in the Enzymatic Synthesis of the Structured Lipid 1,3-Dioleoyl-2-Palmitoylglycerol
Q46161631Impact of orientation and flexibility of peptide linkers on T. maritima lipase Tm1350 displayed on Bacillus subtilis spores surface using CotB as fusion partner.
Q91832350Improved synthesis of isomaltooligosaccharides using immobilized α-glucosidase in organic-aqueous media
Q46839756Improved synthesis of phosphatidylserine using bio-based solvents, limonene and p-cymene
Q33995892Improving the activity and stability of GL-7-ACA acylase CA130 by site-directed mutagenesis
Q84553713In situ enzymatic screening (ISES): a tool for catalyst discovery and reaction development
Q45159152Inactivation of yeast inorganic pyrophosphatase by organic solvents
Q92923821Increasing Performance and Thermostability of D-Phenylglycine Aminotransferase in Miscible Organic Solvents
Q38199980Increasing the activity and enantioselectivity of lipases by sol-gel immobilization: further advancements of practical interest
Q62160378Influence of different silica derivatives in the immobilization and stabilization of a Bacillus licheniformis protease (Subtilisin Carlsberg)
Q35227898Influence of organic solvents on catalytic behaviors and cell morphology of whole-cell biocatalysts for synthesis of 5'-arabinocytosine laurate
Q28398029Inhalation delivery of proteins from ethanol suspensions
Q93154617Inhibition of Pancreatic α-amylase by Resveratrol Derivatives: Biological Activity and Molecular Modelling Evidence for Cooperativity between Viniferin Enantiomers
Q84786945Integration of a Fluorescent Molecular Biosensor into Self‐Assembled Protein Nanowires: A Large Sensitivity Enhancement
Q44107854Interaction of organic solvents with protein structures at protein-solvent interface
Q47393052Interaction of proteins with ionic liquid, alcohol and DMSO and in situ generation of gold nano-clusters in a cell
Q43860078Investigation of spore coat display of Bacillus subtilis β-galactosidase for developing of whole cell biocatalyst
Q34637397Investigations on diffusion limitations of biocatalyzed reactions in amphiphilic polymer conetworks in organic solvents.
Q46567485Ion-ion and ion-molecule reactions at the surface of proteins produced by nanospray. Information on the number of acidic residues and control of the number of ionized acidic and basic residues
Q57663321Ionic liquids microemulsions: the key to Candida antarctica lipase B superactivity
Q28731574Isolation and characterization of EstC, a new cold-active esterase from Streptomyces coelicolor A3(2)
Q35187865Isolation and characterization of a thermotolerant ene reductase from Geobacillus sp. 30 and its heterologous expression in Rhodococcus opacus
Q43028114Isolation and polyphasic characterization of a novel hyper catalase producing thermophilic bacterium for the degradation of hydrogen peroxide
Q50226596Isolation of an organic solvent-tolerant bacterium Bacillus licheniformis PAL05 that is able to secrete solvent-stable lipase
Q51000481Kinetic biosynthesis of L-ascorbyl acetate by immobilized Thermomyces lanuginosus lipase (Lipozyme TLIM).
Q51438321Kinetic investigation of a solvent-free, chemoenzymatic reaction sequence towards enantioselective synthesis of a β-amino acid ester.
Q58655930Kinetic resolutions of racemic amines and alcohols catalyzed by an industrial glutaryl-7-aminocephalosporanic acid acylase with unexpected broad substrate specificity
Q60660283Langmuir–Blodgett films of lipase for biocatalysis
Q47695398Large scale analysis of protein conformational transitions from aqueous to non-aqueous media.
Q42953609Large-scale ruthenium- and enzyme-catalyzed dynamic kinetic resolution of (rac)-1-phenylethanol.
Q41979701Lid opening and conformational stability of T1 Lipase is mediated by increasing chain length polar solvents
Q45990042Lipase from marine strain using cooked sunflower oil waste: production optimization and application for hydrolysis and thermodynamic studies.
Q41971027Lipase in aqueous-polar organic solvents: activity, structure, and stability.
Q43309675Lipase production from a novel thermo-tolerant and extreme acidophile Bacillus pumilus using palm oil as the substrate and treatment of palm oil-containing wastewater
Q34662058Lipases as practical biocatalysts
Q36797644Lipases from extremophiles and potential for industrial applications
Q43003753Lipolytic potential of Aspergillus japonicus LAB01: production, partial purification, and characterisation of an extracellular lipase
Q45165425Liquid poly(ethylene glycol) and supercritical carbon dioxide as a biphasic solvent system for lipase-catalyzed esterification
Q36694649Low operational stability of enzymes in dry organic solvents: changes in the active site might affect catalysis
Q51822561Low-Transition-Temperature Mixtures (LTTMs) for Dissolving Proteins and for Drug Formulation.
Q38747980Lysozyme in water-acetonitrile mixtures: Preferential solvation at the inner edge of excess hydration
Q44177772Lysozyme viscoelastic matrices in tetramethylurea/water media: a small angle X-ray scattering study.
Q52625283Manipulation of the conformation and enzymatic properties of T1 lipase by site-directed mutagenesis of the protein core.
Q30503588Metabolism and motility in prebiotic structures
Q36227783Metagenomic mining for thermostable esterolytic enzymes uncovers a new family of bacterial esterases
Q26827731Microbial enzymes: tools for biotechnological processes
Q35616794Microbiological contributions to the search for extraterrestrial life.
Q46469075Microcalorimetric study of thermal unfolding of lysozyme in water/glycerol mixtures: an analysis by solvent exchange model.
Q34339389Minimizing frustration by folding in an aqueous environment
Q37273478Mining bacterial genomes for novel arylesterase activity
Q58322325Modulating Enzyme Activity by Altering Protein Dynamics with Solvent
Q38623886Molecular Mechanisms Underlying Inhibitory Binding of Alkylimidazolium Ionic Liquids to Laccase
Q45817477Molecular ageing: free radical initiated epimerization of thymopentin--a case study.
Q33825205Molecular cloning and characterization of a new cold-active esterase from a deep-sea metagenomic library.
Q30395513Molecular mechanism of carbon nanotube to activate Subtilisin Carlsberg in polar and non-polar organic media
Q52891881Nanoparticle cages for enzyme catalysis in organic media.
Q104495359Non-aqueous green solvents improve alpha-amylase induced fiber opening in leather processing
Q89242407Non-aqueous homogenous biocatalytic conversion of polysaccharides in ionic liquids using chemically modified glucosidase
Q86512848Non-aqueous suspensions of antibodies are much less viscous than equally concentrated aqueous solutions
Q57087800Novel antifoulants: inhibition of larval attachment by proteases
Q33267540On the activity loss of hydrolases in organic solvents: II. a mechanistic study of subtilisin Carlsberg
Q56948646On the different roles of anions and cations in the solvation of enzymes in ionic liquids
Q58896430On the hunt for truly biocompatible ionic liquids for lipase-catalyzed reactions
Q30353943On the influence of hydrated imidazolium-based ionic liquid on protein structure stability: a molecular dynamics simulation study.
Q44686502On the influence of hydrated ionic liquids on the dynamical structure of model proteins: a computational study
Q57956308On the mechanism of cold denaturation
Q38162172On the nature of interactions between ionic liquids and small amino-acid-based biomolecules.
Q37649051One-pot synthesis of enantiopure syn-1,3-diacetates from racemic syn/anti mixtures of 1,3-diols by dynamic kinetic asymmetric transformation
Q82389298Online capillary liquid-liquid electroextraction of peptides as fast pre-concentration prior to LC-MS
Q34425020Optimization of technological conditions for one-pot synthesis of (S)-alpha-cyano-3-phenoxybenzyl acetate in organic media
Q51287408Organic solvent tolerant lipases and applications.
Q34196630Partial characterization of a novel amphibian hemoglobin as a model for graduate student investigation on peptide chemistry, mass spectrometry, and atomic force microscopy.
Q42153069Penicillin Acylase-Catalyzed Effective and Stereoselective Acylation of 1-phenylethylamine in Aqueous Medium using Non-Activated Acyl Donor.
Q51637033Periplasmic expression as a basis for whole cell kinetic screening of unnatural enzyme reactivities.
Q53323308Perturbation response scanning specifies key regions in subtilisin serine protease for both function and stability.
Q44122480Phosphite dehydrogenase: a versatile cofactor-regeneration enzyme
Q35032037Photonic activation of plasminogen induced by low dose UVB.
Q37083323Plant products for pharmacology: application of enzymes in their transformations
Q90311303Poly (glycerol adipate) (PGA), an Enzymatically Synthesized Functionalizable Polyester and Versatile Drug Delivery Carrier: A Literature Update
Q56927571Polymersome colloidosomes for enzyme catalysis in a biphasic system
Q40117172Predicting enzyme behavior in nonconventional media: correlating nitrilase function with solvent properties
Q57188131Preparation of protein nanocrystals and their characterization by solid state NMR
Q30629561Probing immobilization mechanism of alpha-chymotrypsin onto carbon nanotube in organic media by molecular dynamics simulation
Q36042722Process optimization for production and purification of a thermostable, organic solvent tolerant lipase from Acinetobacter sp. AU07.
Q48106664Production and Biotechnological Application of Extracellular Alkalophilic Lipase from Marine Macroalga-Associated Shewanella algae to Produce Enriched C20-22 n-3 Polyunsaturated Fatty Acid Concentrate.
Q83138487Production of a novel extracellular acidic lipase from Pseudomonas gessardii using slaughterhouse waste as a substrate
Q51140173Protein Stability: Enhancement and Measurement.
Q35842593Protein and peptide parenteral controlled delivery
Q84587100Protein melts: A new phase for protein chemistry
Q30332548Protein structure and dynamics in nonaqueous solvents: insights from molecular dynamics simulation studies.
Q30342632Protein structure, stability and solubility in water and other solvents.
Q33581606Protein thermostability calculations using alchemical free energy simulations
Q51129149Protein-Coated Microcrystals, Combi-Protein-Coated Microcrystals, and Cross-Linked Protein-Coated Microcrystals of Enzymes for Use in Low-Water Media.
Q90543534Protein-polymer bioconjugates via a versatile oxygen tolerant photoinduced controlled radical polymerization approach
Q30402027Proteins in binary solvents
Q50196511Pure Protein Bilayers and Vesicles from Native Fungal Hydrophobins.
Q43015452Purification and characterization of a halophilic α-amylase with increased activity in the presence of organic solvents from the moderately halophilic Nesterenkonia sp. strain F.
Q50053459Purification and characterization of a novel organic solvent-tolerant and cold-adapted lipase from Psychrobacter sp. ZY124.
Q28600843Purification and characterization of endo β-1,4-d-glucanase from Trichoderma harzianum strain HZN11 and its application in production of bioethanol from sweet sorghum bagasse
Q94502031Purification and characterization of thermostable α-amylase from germinating Sword bean (Canavalia gladiata (Jacq.) DC.) seeds
Q43009096Pyrene-Capped CdSe@ZnS Nanoparticles as Sensitive Flexible Oxygen Sensors in Non-Aqueous Media.
Q90547651Quantification of diacylglycerol and triacylglycerol species in human fecal samples by flow injection Fourier transform mass spectrometry
Q38628086Quantitative MS analysis of therapeutic mAbs and their glycosylation for pharmacokinetics study
Q39201958Radiofrequency treatment enhances the catalytic function of an immobilized nanobiohybrid catalyst
Q80492532Rapid screening of enzymes for the enzymatic hydrolysis of chiral esters in drug discovery
Q26782791Recent Developments in Chemical Synthesis with Biocatalysts in Ionic Liquids
Q30409364Reflections on charge state distributions, protein structure, and the mystical mechanism of electrospray ionization
Q56036119Regioselective Deacetylation of Disaccharides via ImmobilizedAspergillus nigerEsterase(s)-catalyzed Hydrolysis in Aqueous and Non-aqueous Media
Q51655955Removing the Active-Site Flap in Lipase A from Candida antarctica Produces a Functional Enzyme without Interfacial Activation.
Q42177214Resolving anisotropic distributions of correlated vibrational motion in protein hydration water
Q51771189Reversible increase in the redox potential of cytochrome c in methanol.
Q41826779Role of methoxypolyethylene glycol on the hydration, activity, conformation and dynamic properties of a lipase in a dry film.
Q43029429Screening, purification, and characterization of a novel organic solvent-tolerant esterase, Lip2, from Monascus purpureus strain M7.
Q35131940Short-chain flavor ester synthesis in organic media by an E. coli whole-cell biocatalyst expressing a newly characterized heterologous lipase
Q94503640Significantly Enhanced Synthesis of Aromatic Esters of Arbutin Catalyzed by Immobilized Lipase in Co-solvent Systems
Q36173765Site-specific hydration dynamics of globular proteins and the role of constrained water in solvent exchange with amphiphilic cosolvents.
Q36288188Site-specific measurement of water dynamics in the substrate pocket of ketosteroid isomerase using time-resolved vibrational spectroscopy
Q43923938Size as a parameter for solvent effects on Candida antarctica lipase B enantioselectivity
Q52682431Solid-phase synthesis of protein-polymers on reversible immobilization supports.
Q45058318Solid-state nanopore analysis of alcohol-soluble molecules
Q51269628Solvent engineering substantially enhances the chemoenzymatic production of surfactin.
Q26771170Solvents and sustainable chemistry
Q60960514Solvents to Fragments to Drugs: MD Applications in Drug Design
Q57623582Spectroscopic Characterization and Electrochemistry of Poly(ethylene oxide)-Modified Myoglobin in Organic Solvents
Q57368531Sponge-like ionic liquids: a new platform for green biocatalytic chemical processes
Q36217972Stabilization of proteins in solid form
Q57390190State-of-the-Art
Q28661877Strategies for discovery and improvement of enzyme function: state of the art and opportunities
Q41246214Structural and biochemical characterisation of Archaeoglobus fulgidus esterase reveals a bound CoA molecule in the vicinity of the active site
Q42649853Structural determinants of ligand imprinting: a molecular dynamics simulation study of subtilisin in aqueous and apolar solvents
Q45186088Structure and dynamics of Candida rugosa lipase: the role of organic solvent.
Q38219155Sugar ester surfactants: enzymatic synthesis and applications in food industry
Q30779121Suitability of binary mixtures of water with aprotic solvents to turn hydroxyl protons of carbohydrate ligands into conformational sensors in NOE and transferred NOE experiments
Q42037844Supercritical carbon dioxide and its potential as a life-sustaining solvent in a planetary environment
Q47835938Supramolecular Storage and Controlled Photorelease of an Oxidizing Agent using a Bambusuril Macrocycle.
Q43129729Surfactant-stabilized small hydrogel particles in oil: hosts for remarkable activation of enzymes in organic solvents
Q50890445Sustained gastrointestinal activity of dendronized polymer-enzyme conjugates.
Q43031040Synergistic hydrolysis of xylan using novel xylanases, β-xylosidases, and an α-L-arabinofuranosidase from Geobacillus thermodenitrificans NG80-2.
Q42133994Synthesis of chiral cyanohydrins by recombinant Escherichia coli cells in a micro-aqueous reaction system
Q55116628Synthesis of designer triglycerides by enzymatic acidolysis.
Q49692144Synthetically useful variants of industrial lipases from Burkholderia cepacia and Pseudomonas fluorescens
Q43176735Tailored adsorption of His6-tagged protein onto nickel(II)-cyclam grafted mesoporous silica
Q100738549Tailoring Particle-enzyme Nanoconjugates for Biocatalysis at the Organic-organic Interface
Q50944660Temperature-responsive enzyme-polymer nanoconjugates with enhanced catalytic activities in organic media.
Q43808630The NADH oxidase from Pyrococcus furiosus. Implications for the protection of anaerobic hyperthermophiles against oxidative stress.
Q52607524The effect of solvent on the catalytic properties of microperoxidase-11.
Q81589606The effect of ultrasound on lipase-catalyzed hydrolysis of soy oil in solvent-free system
Q55108934The fate of proteins in outer space.
Q28301006The prospect of alien life in exotic forms on other worlds
Q46756136The structure and synthetic capabilities of a catalytic peptide formed by substrate-directed mechanism--implications to prebiotic catalysis
Q31035902The thioesterase domain from a nonribosomal peptide synthetase as a cyclization catalyst for integrin binding peptides
Q30431271Thermal unfolding and refolding of lysozyme in deep eutectic solvents and their aqueous dilutions.
Q26781363Thermophilic and alkaliphilic Actinobacteria: biology and potential applications
Q54271984Thermostability and reactivity in organic solvent of O-phospho-L-serine sulfhydrylase from hyperthermophilic archaeon Aeropyrum pernix K1.
Q30390403To Keep or Not to Keep? The Question of Crystallographic Waters for Enzyme Simulations in Organic Solvent.
Q59133775Tomato GDSL1 is required for cutin deposition in the fruit cuticle
Q47660095Transesterification Synthesis of Chloramphenicol Esters with the Lipase from Bacillus amyloliquefaciens
Q90781445Transforming protein-polymer conjugate purification by tuning protein solubility
Q35759315Transgalactosylation in a water-solvent biphasic reaction system with beta-galactosidase displayed on the surfaces of Bacillus subtilis spores.
Q28703562Transporter engineering for improved tolerance against alkane biofuels in Saccharomyces cerevisiae
Q44815632Trypsin-catalyzed kinetically controlled synthesis of a precursor dipeptide of thymopentin in organic solvents, using a free amino acid as nucleophile
Q43713524Tuning of the product spectrum of vanillyl-alcohol oxidase by medium engineering
Q50428416Ultrasound enhances lipase-catalyzed synthesis of poly (ethylene glutarate).
Q56334665Ultrasound-assisted biosynthesis of novel methotrexate-conjugates
Q89069503Ultrasound-assisted improvements in biocatalytic activity and production of organic-solvent stable protease from Bacillus circulans MTCC 7942
Q80525242Unexpectedly enhanced stereoselectivity of peroxidase-catalyzed sulfoxidation in branched alcohols
Q38684776Unscrambling micro-solvation of -COOH and -NH groups in neat dimethyl sulfoxide: insights from 1H-NMR spectroscopy and computational studies
Q39231051Upgrading Laccase Production and Biochemical Properties: Strategies and Challenges.
Q57378209Urease-Dextran complexes with enhanced enzymatic activity and stability
Q44062203Use of isolated cyclohexanone monooxygenase from recombinant Escherichia coli as a biocatalyst for Baeyer-Villiger and sulfide oxidations.
Q34346895Using ionic liquids in whole-cell biocatalysis for the nucleoside acylation
Q31096633Water Determines the Structure and Dynamics of Proteins
Q39156187Water Dynamics in the Hydration Shells of Biomolecules
Q41764552Water dependent properties of cutinase in nonaqueous solvents: a computational study of enantioselectivity
Q33238563Water dynamics and salt-activation of enzymes in organic media: mechanistic implications revealed by NMR spectroscopy
Q42740070Water dynamics in protein hydration shells: the molecular origins of the dynamical perturbation
Q90001574Water follows polar and nonpolar protein surface domains
Q57124930Water miscible mono alcohols' effect on the proteolytic performance of Bacillus clausii serine alkaline protease
Q77589803Water sorption isotherms and enthalpies of water sorption by lysozyme using the quartz crystal microbalance/heat conduction calorimeter
Q35214071What can we learn by studying enzymes in non-aqueous media?
Q37130180Whole cell microbial transformation in cloud point system
Q60930213Xylan deterioration approach: Purification and catalytic behavior optimization of a novel β-1,4-d-xylanohydrolase from KIBGE-IB29
Q38726560α-chymotrypsin in water-acetone and water-dimethyl sulfoxide mixtures: Effect of preferential solvation and hydration.