Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype, and components of the type 2 Shiga toxin family by multiplex PCR.

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Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype, and components of the type 2 Shiga toxin family by multiplex PCR. is …
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scholarly articleQ13442814

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P356DOI10.1128/JCM.40.10.3613-3619.2002
P932PMC publication ID130888
P698PubMed publication ID12354854

P2093author name stringClifford G Clark
Frank G Rodgers
Gehua Wang
P2860cites workThe Epidemiology of Infections Caused by Escherichia coli O157: H7, Other Enterohemorrhagic E. coli, and the Associated Hemolytic Uremic SyndromeQ33375970
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Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157.Q33501643
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Sequence diversity of the Escherichia coli H7 fliC genes: implication for a DNA-based typing scheme for E. coli O157:H7Q33966837
Molecular cloning and nucleotide sequence of another variant of the Escherichia coli Shiga-like toxin II familyQ34050888
Escherichia coli ehl1 gene-positive serotype O18ac:H31 associated with an outbreak of diarrhea in a neonatal nursery in Neuquén City, ArgentinaQ34455886
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The specific activities of Shiga-like toxin type II (SLT-II) and SLT-II-related toxins of enterohemorrhagic Escherichia coli differ when measured by Vero cell cytotoxicity but not by mouse lethalityQ35773392
Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swineQ36215407
Nucleotide sequence and promoter mapping of the Escherichia coli Shiga-like toxin operon of bacteriophage H-19B.Q36265701
Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strainsQ36543530
Molecular characterization of the gene encoding H antigen in Escherichia coli and development of a PCR-restriction fragment length polymorphism test for identification of E. coli O157:H7 and O157:NMQ36575087
Two copies of Shiga-like toxin II-related genes common in enterohemorrhagic Escherichia coli strains are responsible for the antigenic heterogeneity of the O157:H- strain E32511Q36974749
Differentiation of genes coding for Escherichia coli verotoxin 2 and the verotoxin associated with porcine edema disease (VTe) by the polymerase chain reaction.Q37191358
Identification of verotoxin type 2 variant B subunit genes in Escherichia coli by the polymerase chain reaction and restriction fragment length polymorphism analysisQ37239269
Multiplex PCRs for identification of Escherichia coli virulence genesQ39457606
A new Shiga toxin 2 variant (Stx2f) from Escherichia coli isolated from pigeons.Q39485510
Identification of new verocytotoxin type 2 variant B-subunit genes in human and animal Escherichia coli isolatesQ39556186
Comparative toxicity and virulence of Escherichia coli clones expressing variant and chimeric Shiga-like toxin type II operonsQ39822350
Use of digoxigenin-labelled oligonucleotide DNA probes for VT2 and VT2 human variant genes to differentiate Vero cytotoxin-producing Escherichia coli strains of serogroup O157.Q40188030
Development of verotoxin 2- and verotoxin 2 variant (VT2v)-specific oligonucleotide probes on the basis of the nucleotide sequence of the B cistron of VT2v from Escherichia coli E32511 and B2F1Q40214420
Cloning and nucleotide sequence analysis of the genes determining verocytotoxin production in a porcine edema disease isolate of Escherichia coliQ42653384
Cloning and nucleotide sequence of a variant Shiga-like toxin II gene from Escherichia coli OX3:H21 isolated from a case of sudden infant death syndromeQ48160670
A multiplex polymerase chain reaction assay for rapid detection and identification of Escherichia coli O157:H7 in foods and bovine feces.Q50509991
A multiplex PCR for identifying Shiga-like toxin-producing Escherichia coli O157:H7.Q54570060
Specific identification of Escherichia coli O157:H7 using a multiplex PCR assay.Q54573630
A cloned pathogenicity island from enteropathogenic Escherichia coli confers the attaching and effacing phenotype on E. coli K-12.Q54574143
Amplification by the polymerase chain reaction of a specific target sequence in the gene coding for Escherichia coli verotoxin (VTe variant)Q68331006
Detection of various variant verotoxin genes in Escherichia coli by polymerase chain reactionQ72584167
Multiplex PCR for detection of trait and virulence factors in enterohemorrhagic Escherichia coli serotypesQ73220571
Rapid and sensitive detection of Escherichia coli O157:H7 in bovine faeces by a multiplex PCRQ73423973
Characterization of shiga toxin type 2 variant B-subunit in Escherichia coli strains from asymptomatic human carriers by PCR-RFLPQ74266754
Virulence genes of Shiga toxin-producing Escherichia coli isolated from food, animals and humansQ77931726
P433issue10
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
P304page(s)3613-3619
P577publication date2002-10-01
P1433published inJournal of Clinical MicrobiologyQ4041880
P1476titleDetection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype, and components of the type 2 Shiga toxin family by multiplex PCR.
P478volume40

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