Use of fluorescent techniques to study the in vitro movement of myosins

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Use of fluorescent techniques to study the in vitro movement of myosins is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1007/978-3-0348-0856-9_9
P932PMC publication ID4178934
P698PubMed publication ID25095996

P50authorChristopher N ToepferQ59536414
P2093author name stringJames R Sellers
P2860cites workDirect observation of molecular motility by light microscopyQ67950317
Factors affecting movement of F-actin filaments propelled by skeletal muscle heavy meromyosinQ68077086
Polarity and velocity of sliding filaments: control of direction by actin and of speed by myosinQ68870306
Characterization of in vitro motility assays using smooth muscle and cytoplasmic myosinsQ68905336
In Vitro Motility Analysis of Smooth Muscle Caldesmon Control of Actin-Tropomyosin Filament MovementQ71996652
In vitro motility analysis of actin-tropomyosin regulation by troponin and calcium. The thin filament is switched as a single cooperative unitQ72149260
Smooth, cardiac and skeletal muscle myosin force and motion generation assessed by cross-bridge mechanical interactions in vitroQ72417450
The role of water in the mechanism of muscular contractionQ72798802
Direct observation of processive movement by individual myosin V moleculesQ73838381
Kinetic tuning of myosin via a flexible loop adjacent to the nucleotide binding pocketQ74299321
Loop I can modulate ADP affinity, ATPase activity, and motility of different scallop myosins. Transient kinetic analysis of S1 isoformsQ74540838
Regulation of myosin V processivity by calcium at the single molecule levelQ80141852
Myosin VI steps via a hand-over-hand mechanism with its lever arm undergoing fluctuations when attached to actinQ80341101
Full-length myosin VI dimerizes and moves processively along actin filaments upon monomer clusteringQ82457987
Drawing the tree of eukaryotic life based on the analysis of 2,269 manually annotated myosins from 328 speciesQ21092862
Myosin VI is an actin-based motor that moves backwardsQ22010643
In vitro reconstitution of a transport complex containing Rab27a, melanophilin and myosin VaQ24307247
Skeletal muscle expression and abnormal function of beta-myosin in hypertrophic cardiomyopathyQ24309554
Myosin VI is a processive motor with a large step sizeQ24555089
Actin structure-dependent stepping of myosin 5a and 10 during processive movementQ27306218
The yeast class V myosins, Myo2p and Myo4p, are nonprocessive actin-based motorsQ27935400
Structure of the actin-myosin complex and its implications for muscle contractionQ28259888
Altered cardiac troponin T in vitro function in the presence of a mutation implicated in familial hypertrophic cardiomyopathyQ28283047
Myosin V: regulation by calcium, calmodulin, and the tail domainQ28588566
Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localizationQ29615477
Actin, a central player in cell shape and movementQ29616426
Adaptability of myosin V studied by simultaneous detection of position and orientationQ30477232
Dimerized Drosophila myosin VIIa: a processive motorQ30477237
Myosin Va maneuvers through actin intersections and diffuses along microtubulesQ30479183
Myosin V and Kinesin act as tethers to enhance each others' processivityQ30481620
A myosin motor that selects bundled actin for motilityQ30482736
Unconventional myosin traffic in cells reveals a selective actin cytoskeletonQ30488428
Single-molecule stepping and structural dynamics of myosin X.Q30494681
Structured post-IQ domain governs selectivity of myosin X for fascin-actin bundlesQ30496161
The light chain binding domain of expressed smooth muscle heavy meromyosin acts as a mechanical leverQ30652709
Differential labeling of myosin V heads with quantum dots allows direct visualization of hand-over-hand processivityQ33212840
The molecular basis of frictional loads in the in vitro motility assay with applications to the study of the loaded mechanochemistry of molecular motorsQ33535276
Myosins: a diverse superfamilyQ33866577
In vitro actin filament sliding velocities produced by mixtures of different types of myosinQ33915322
The Stepping Pattern of Myosin X Is Adapted for Processive Motility on Bundled ActinQ34134758
Nanometer localization of single green fluorescent proteins: evidence that myosin V walks hand-over-hand via telemark configurationQ34187072
Development and use of fluorescent protein markers in living cellsQ34188528
Walking to work: roles for class V myosins as cargo transportersQ34238805
Direct observation of motion of single F-actin filaments in the presence of myosinQ34263051
Movement of myosin-coated fluorescent beads on actin cables in vitroQ34266105
Single myosin molecule mechanics: piconewton forces and nanometre stepsQ34338098
The cargo-binding domain regulates structure and activity of myosin 5Q35752269
Engineered myosin VI motors reveal minimal structural determinants of directionality and processivity.Q35990181
Collective dynamics of elastically coupled myosin V motorsQ36201901
Calmodulin dissociation regulates brush border myosin I (110-kD-calmodulin) mechanochemical activity in vitroQ36222736
Walking with myosin V.Q36354001
Kinetic characterization of nonmuscle myosin IIb at the single molecule levelQ36508784
Myosin VI has a one track mind versus myosin Va when moving on actin bundles or at an intersectionQ36542334
Molecular mechanics of cardiac myosin-binding protein C in native thick filamentsQ36579217
Tropomyosin is essential for processive movement of a class V myosin from budding yeastQ36604050
Temperature dependent measurements reveal similarities between muscle and non-muscle myosin motilityQ36883114
Nonmuscle myosin IIA with a GFP fused to the N-terminus of the regulatory light chain is regulated normallyQ37205755
The tail binds to the head-neck domain, inhibiting ATPase activity of myosin VIIAQ37208900
More than just a cargo adapter, melanophilin prolongs and slows processive runs of myosin VaQ37226089
Cargo binding induces dimerization of myosin VI.Q37366472
Coupled myosin VI motors facilitate unidirectional movement on an F-actin networkQ37387839
Fluorescent actin filaments move on myosin fixed to a glass surfaceQ37396490
Direct observation of the mechanochemical coupling in myosin Va during processive movementQ37416808
A mechanochemical mechanism for muscle contractionQ37472279
The SAH domain extends the functional length of the myosin leverQ37477144
Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC).Q39994903
The globular tail domain of myosin Va functions as an inhibitor of the myosin Va motorQ40270903
A monomeric myosin VI with a large working strokeQ40573331
Myosin-specific adaptations of the motility assay.Q40782881
Actin-binding proteins regulate the work performed by myosin II motors on single actin filamentsQ41110131
Myosin VI walks "wiggly" on actin with large and variable tiltingQ42149706
Inhibition of the relative movement of actin and myosin by caldesmon and calponinQ43564183
Neck length and processivity of myosin V.Q44436395
Step-size is determined by neck length in myosin V.Q47358625
Identification of the single specific IQ motif of myosin V from which calmodulin dissociates in the presence of Ca2+.Q48419013
Enzymatic characterization and functional domain mapping of brain myosin-V.Q48962038
Phospholipid-dependent regulation of the motor activity of myosin X.Q52612413
The predicted coiled-coil domain of myosin 10 forms a novel elongated domain that lengthens the head.Q52857621
Myosin subfragment-1 is sufficient to move actin filaments in vitroQ59053550
Sliding movement of single actin filaments on one-headed myosin filamentsQ59068962
[33] Assays for actin sliding movement over myosin-coated surfacesQ63383962
P304page(s)193-210
P577publication date2014-01-01
P1433published inEXSQ27709924
P1476titleUse of fluorescent techniques to study the in vitro movement of myosins
P478volume105

Reverse relations

cites work (P2860)
Q48050711Actomyosin interaction at low ATP concentrations
Q33810384Myosin Va's adaptor protein melanophilin enforces track selection on the microtubule and actin networks in vitro.

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