Purification and reconstitution of functional lactose carrier from Escherichia coli.

scientific article

Purification and reconstitution of functional lactose carrier from Escherichia coli. is …
instance of (P31):
scholarly articleQ13442814

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P698PubMed publication ID7028742

P50authorHoward Ronald KabackQ1562621
P2093author name stringKaback HR
Foster DL
Wilson TH
Newman MJ
P433issue22
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
P1104number of pages5
P304page(s)11804-11808
P577publication date1981-11-01
P1433published inJournal of Biological ChemistryQ867727
P1476titlePurification and reconstitution of functional lactose carrier from Escherichia coli
P478volume256

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cites work (P2860)
Q26995227A retrospective: use of Escherichia coli as a vehicle to study phospholipid synthesis and function
Q38309375A suppressor analysis of residues involved in cation transport in the lactose permease: identification of a coupling sensor
Q36282878Alkalophilic Bacillus firmus RAB generates variants which can grow at lower Na+ concentrations than the parental strain
Q37056022Anion-exchange mechanisms in bacteria.
Q38344569Binding of monoclonal antibody 4B1 to homologs of the lactose permease of Escherichia coli
Q45263507Bioenergetics of alkalophilic bacteria
Q42166449Characteristics of n-octyl β-d-thioglucopyranoside, a new non-ionic detergent useful for membrane biochemistry
Q42141434Characterization of the 70 kDa polypeptide of the Na/Ca exchanger
Q34426779Characterization of the thyroid Na+/I- symporter with an anti-COOH terminus antibody
Q39965717Cloning of the tyrP gene and further characterization of the tyrosine-specific transport system in Escherichia coli K-12
Q24814923Comparative analyses of fundamental differences in membrane transport capabilities in prokaryotes and eukaryotes
Q39975458Comparison of lactose uptake in resting and energized Escherichia coli cells: high rates of respiration inactivate the lac carrier.
Q35641812Conformational flexibility at the substrate binding site in the lactose permease of Escherichia coli
Q38318851Cysteine scanning mutagenesis of putative transmembrane helices IX and X in the lactose permease of Escherichia coli
Q38345138Dependence on pH of substrate binding to a mutant lactose carrier, lacYun, in Escherichia coli. A model for H+/lactose symport
Q37704678Design of a membrane transport protein for fluorescence spectroscopy
Q33404282Detergent binding explains anomalous SDS-PAGE migration of membrane proteins
Q34318277Detergent disruption of bacterial inner membranes and recovery of protein translocation activity
Q69905388Effect of different phospholipids on the reconstitution of two functions of the lactose carrier of Escherichia coli
Q36405049Effect of silver ions on transport and retention of phosphate by Escherichia coli.
Q37357814Effects of impurities on membrane-protein crystallization in different systems
Q37164425Electrophysiological characterization of LacY.
Q36254287Energy coupling in bacterial periplasmic permeases
Q47176089Evidence for high molecular weight Na-Ca exchange in cardiac sarcolemmal vesicles
Q41947432Exploring the world of phospholipids and their interactions with proteins: the work of William Dowhan
Q38289728Fluorescence of native single-Trp mutants in the lactose permease from Escherichia coli: structural properties and evidence for a substrate-induced conformational change
Q36845441Functional complementation of internal deletion mutants in the lactose permease of Escherichia coli
Q36248757Functional molecular weight of the lac carrier protein from Escherichia coli as studied by radiation inactivation analysis
Q89930646H. Ronald Kaback 1936-2019
Q33891527Helix packing of lactose permease in Escherichia coli studied by site-directed chemical cleavage
Q37574581Identification and partial purification of the cardiac sodium-calcium exchange protein
Q33618213In vivo expression of the lacY gene in two segments leads to functional lac permease
Q35996552Inhibition of human GLUT1 and GLUT5 by plant carbohydrate products; insights into transport specificity
Q37560344Intramolecular dislocation of the COOH terminus of the lac carrier protein in reconstituted proteoliposomes
Q37691170Isolation and nucleotide sequencing of lactose carrier mutants that transport maltose
Q92418260It takes two to tango: The dance of the permease
Q35629460Lactose carrier mutants of Escherichia coli with changes in sugar recognition (lactose versus melibiose)
Q36475370Lessons from lactose permease
Q40141191Mechanism, regulation and physiological significance of the loop diuretic-sensitive NaCl/KCl symport system in animal cells
Q36425698Molecular cloning of the phosphate (inorganic) transport (pit) gene of Escherichia coli K12. Identification of the pit+ gene product and physical mapping of the pit-gor region of the chromosome.
Q34213970Opening the periplasmic cavity in lactose permease is the limiting step for sugar binding
Q42653914Phospholipid-assisted protein folding: phosphatidylethanolamine is required at a late step of the conformational maturation of the polytopic membrane protein lactose permease
Q39965880Polypeptide involved in the Escherichia coli plasmid-mediated citrate transport system
Q36320486Preparation, characterization, and properties of monoclonal antibodies against the lac carrier protein from Escherichia coli
Q35454494Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli
Q37055169Protein phosphorylation and allosteric control of inducer exclusion and catabolite repression by the bacterial phosphoenolpyruvate: sugar phosphotransferase system.
Q36125379Protein secretion in Pseudomonas aeruginosa: the xcpA gene encodes an integral inner membrane protein homologous to Klebsiella pneumoniae secretion function protein PulO
Q42873690Proteoliposomes as tool for assaying membrane transporter functions and interactions with xenobiotics
Q36672016Proton-dependent multidrug efflux systems
Q37896714Proton-linked sugar transport systems in bacteria
Q45913430Proton-solute coupling mechanism of the maltose transporter from Saccharomyces cerevisiae.
Q36255042Purified reconstituted lac carrier protein from Escherichia coli is fully functional
Q34303297Reconstitution of a bacterial periplasmic permease in proteoliposomes and demonstration of ATP hydrolysis concomitant with transport
Q37345325Reconstitution of active transport in proteoliposomes containing cytochrome o oxidase and lac carrier protein purified from Escherichia coli
Q37447578Sequence alignment and homology threading reveals prokaryotic and eukaryotic proteins similar to lactose permease
Q36747253Single gene deletions of mrpA to mrpG and mrpE point mutations affect activity of the Mrp Na+/H+ antiporter of alkaliphilic Bacillus and formation of hetero-oligomeric Mrp complexes
Q30478719Site-directed alkylation and the alternating access model for LacY.
Q37327186Site-directed spin labeling and chemical crosslinking demonstrate that helix V is close to helices VII and VIII in the lactose permease of Escherichia coli
Q37534919Site-specific mutagenesis of histidine residues in the lac permease of Escherichia coli
Q83271775Solubilization, partial purification, and reconstitution of the glycolate/glycerate transporter from chloroplast inner envelope membranes
Q37612695Structural bases for the interaction and stabilization of the human amino acid transporter LAT2 with its ancillary protein 4F2hc.
Q30361148Substrate-induced changes in the structural properties of LacY
Q36942219The N-terminal 22 amino acid residues in the lactose permease of Escherichia coli are not obligatory for membrane insertion or transport activity
Q35191643The central cytoplasmic loop of the major facilitator superfamily of transport proteins governs efficient membrane insertion
Q36210605The ndvA gene product of Rhizobium meliloti is required for beta-(1----2)glucan production and has homology to the ATP-binding export protein HlyB.
Q42845778The role of helix VIII in the lactose permease of Escherichia coli: I. Cys-scanning mutagenesis
Q41845257The role of helix VIII in the lactose permease of Escherichia coli: II. Site-directed sulfhydryl modification
Q42844021The role of transmembrane domain III in the lactose permease of Escherichia coli
Q41432949The size of the lactose permease derived from rotational diffusion measurements
Q42107611The transient kinetics of uptake of galactosides into Escherichia coli
Q40163942TheLac carrier protein inEscherichia coli
Q34206680Topology of polytopic membrane protein subdomains is dictated by membrane phospholipid composition
Q37613547Topology of the lac carrier protein in the membrane of Escherichia coli
Q35342512Toward rational design of protein detergent complexes: determinants of mixed micelles that are critical for the in vitro stabilization of a G-protein coupled receptor.
Q70369350Transport by reconstituted lactose carrier from parental and mutant strains of Escherichia coli
Q39953416Truncated forms of Escherichia coli lactose permease: models for study of biosynthesis and membrane insertion
Q39296619Understanding phospholipid function: Why are there so many lipids?
Q40734067What's new with lactose permease.
Q88793098ZMPSTE24 missense mutations that cause progeroid diseases decrease prelamin A cleavage activity and/or protein stability

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