Towards a pathogenic Escherichia coli detection platform using multiplex SYBR®Green Real-time PCR methods and high resolution melting analysis.

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Towards a pathogenic Escherichia coli detection platform using multiplex SYBR®Green Real-time PCR methods and high resolution melting analysis. is …
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scholarly articleQ13442814

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P819ADS bibcode2012PLoSO...739287K
P356DOI10.1371/JOURNAL.PONE.0039287
P932PMC publication ID3382608
P698PubMed publication ID22761753
P5875ResearchGate publication ID228116490

P50authorDafni Maria KagkliQ57448884
Silvia FolloniQ79456533
P2093author name stringElodie Barbau-Piednoir
Marc Van den Bulcke
Guy Van den Eede
P2860cites workProspective genomic characterization of the German enterohemorrhagic Escherichia coli O104:H4 outbreak by rapid next generation sequencing technologyQ21091008
Enteroaggregative Escherichia coli: An Emerging Enteric Food Borne PathogenQ21328680
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Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infectionsQ24550740
Genome sequence analyses of two isolates from the recent Escherichia coli outbreak in Germany reveal the emergence of a new pathotype: Entero-Aggregative-Haemorrhagic Escherichia coli (EAHEC)Q24624844
A new health threat in Europe: Shiga toxin-producing Escherichia coli O104:H4 infectionsQ26862655
Crystal structure of the cell-binding B oligomer of verotoxin-1 from E. coliQ27644365
Detection of Escherichia coli serogroup O103 by real-time polymerase chain reactionQ31160733
Phylogenetic analysis of Shiga toxin 1 and Shiga toxin 2 genes associated with disease outbreaksQ33308321
Escherichia coli harboring Shiga toxin 2 gene variants: frequency and association with clinical symptomsQ33340303
Shiga toxin 1c-producing Escherichia coli strains: phenotypic and genetic characterization and association with human diseaseQ33348352
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Escherichia coli O157.Q33392428
Epidemic profile of Shiga-toxin-producing Escherichia coli O104:H4 outbreak in GermanyQ33395886
Escherichia coli O104, Germany 2011.Q33395931
High-resolution melting system to perform multilocus sequence typing of Campylobacter jejuniQ33812806
Virulence gene profiling of enterohemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli strains: a basis for molecular risk assessment of typical and atypical EPEC strainsQ33937460
Evolution of virulence factors in Shiga-toxin-producing Escherichia coliQ34158603
Vero response to a cytotoxin of Escherichia coliQ34177128
Detection and characterization of verocytotoxin-producing Escherichia coli by automated 5' nuclease PCR assayQ35155869
Application of the modular approach to an in-house validation study of real-time PCR methods for the detection and serogroup determination of verocytotoxigenic Escherichia coli.Q35271625
Nucleotide sequence and promoter mapping of the Escherichia coli Shiga-like toxin operon of bacteriophage H-19B.Q36265701
Shiga toxin-producing Escherichia coli: an overviewQ36645406
Detection of diarrheagenic Escherichia coli by use of melting-curve analysis and real-time multiplex PCR.Q36672988
Verocytotoxin-producing Escherichia coli (VTEC).Q37467549
Shiga toxins--from cell biology to biomedical applicationsQ37659792
Non-O157 Shiga toxin-producing Escherichia coli in foodsQ37786659
Relevance in pathogenesis researchQ37877014
Rapid detection and identification of clinically important bacteria by high-resolution melting analysis after broad-range ribosomal RNA real-time PCR.Q44441817
Diagnostic method validation: High resolution melting (HRM) of small amplicons genotyping for the most common variants in the MTHFR gene.Q46019062
Characteristics of Shiga toxin-producing Escherichia coli from meat and milk products of different origins and association with food producing animals as main contamination sourcesQ46844848
Technical specifications for the monitoring and reporting of verotoxigenic Escherichia coli (VTEC) on animals and food (VTEC surveys on animals and food)Q47147663
Detection by 5'-nuclease PCR of Shiga-toxin producing Escherichia coli O26, O55, O91, O103, O111, O113, O145 and O157:H7, associated with the world's most frequent clinical casesQ47625876
Detection of stx1, stx2, and eae genes of enterohemorrhagic Escherichia coli using SYBR Green in a real-time polymerase chain reactionQ48018244
Rapid differentiation of bacterial species by high resolution melting curve analysis.Q54356366
Rapid differentiation of bacterial species by high resolution melting curve analysisQ58862206
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue6
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
P304page(s)e39287
P577publication date2012-06-25
P1433published inPLOS OneQ564954
P1476titleTowards a pathogenic Escherichia coli detection platform using multiplex SYBR®Green Real-time PCR methods and high resolution melting analysis
P478volume7

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cites work (P2860)
Q37658631A review on detection methods used for foodborne pathogens
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Q40182094Development of a multiplex qPCR in real time for quantification and differential diagnosis of Salmonella Gallinarum and Salmonella Pullorum
Q43312314Evaluation of a multiplex real-time PCR method for detecting shiga toxin-producing Escherichia coli in beef and comparison to the U.S. Department of Agriculture Food Safety and Inspection Service Microbiology laboratory guidebook method
Q33628277Frequency of shiga toxin-producing genes of Escherichia coli isolated from diarrheic stools of Iranian children by PCR
Q36372444Identification and quantification of virulence factors of enterotoxigenic Escherichia coli by high-resolution melting curve quantitative PCR.
Q42372831Molecular Characterization of Diarrheagenic Escherichia Coli in Children Less Than 5 Years of Age with Diarrhea in Ouagadougou, Burkina Faso.
Q91970000Multiplex Real-Time PCR with HRM for Detection of Lactobacillus sakei and Lactobacillus curvatus in Food Samples
Q38602483Plasmid-based controls to detect rpoB mutations in Mycobacterium tuberculosis by quantitative polymerase chain reaction-high-resolution melting