Engineering Yarrowia lipolytica to produce glycoproteins homogeneously modified with the universal Man3GlcNAc2 N-glycan core

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Engineering Yarrowia lipolytica to produce glycoproteins homogeneously modified with the universal Man3GlcNAc2 N-glycan core is …
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scholarly articleQ13442814

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P819ADS bibcode2012PLoSO...739976D
P356DOI10.1371/JOURNAL.PONE.0039976
P932PMC publication ID3386995
P698PubMed publication ID22768188
P5875ResearchGate publication ID228331781

P50authorNico CallewaertQ49041235
P2093author name stringSteven Geysens
Annelies Van Hecke
Karen De Pourcq
Petra Tiels
Wouter Vervecken
P2860cites workCharacterization of a cDNA encoding a novel human Golgi alpha 1, 2-mannosidase (IC) involved in N-glycan biosynthesisQ22254696
Molecular cloning, chromosomal mapping and tissue-specific expression of a novel human alpha1,2-mannosidase gene involved in N-glycan maturationQ24317680
Molecular cloning and primary structure of Man9-mannosidase from human kidneyQ24317785
A classification of glycosyl hydrolases based on amino acid sequence similaritiesQ24527325
STT3, a highly conserved protein required for yeast oligosaccharyl transferase activity in vivoQ24598071
Cloning and characterization of the ALG3 gene of Saccharomyces cerevisiaeQ27930291
Ordered assembly of the asymmetrically branched lipid-linked oligosaccharide in the endoplasmic reticulum is ensured by the substrate specificity of the individual glycosyltransferasesQ27932071
Yeast mutants deficient in protein glycosylationQ27933198
Yeast GTB1 encodes a subunit of glucosidase II required for glycoprotein processing in the endoplasmic reticulumQ27933980
A novel role for Gtb1p in glucose trimming of N-linked glycans.Q27935261
Substrate specificity analysis of endoplasmic reticulum glucosidase II using synthetic high mannose-type glycans.Q51804438
New disruption cassettes for rapid gene disruption and marker rescue in the yeast Yarrowia lipolytica.Q52552456
Deletion of the glucosidase II gene in Trypanosoma brucei reveals novel N-glycosylation mechanisms in the biosynthesis of variant surface glycoprotein.Q53660014
Glycoprotein biosynthesis in the alg3 Saccharomyces cerevisiae mutant. II. Structure of novel Man6-10GlcNAc2 processing intermediates on secreted invertase.Q54238091
Glycoprotein biosynthesis in the alg3 Saccharomyces cerevisiae mutant. I. Role of glucose in the initial glycosylation of invertase in the endoplasmic reticulum.Q54238093
Structure of Saccharomyces cerevisiae alg3, sec18 mutant oligosaccharides.Q54299841
Identification of the gene encoding the alpha1,3-mannosyltransferase (ALG3) in Arabidopsis and characterization of downstream n-glycan processing.Q54527862
Analysis ofYarrowia lipolyticaextracellular lipase Lip2p glycosylationQ58381924
Glucosylated free oligosaccharides are biomarkers of endoplasmic- reticulum α-glucosidase inhibitionQ63363519
Inhibition of N-linked complex oligosaccharide formation by 1-deoxynojirimycin, an inhibitor of processing glucosidasesQ72769768
Strong hybrid promoters and integrative expression/secretion vectors for quasi-constitutive expression of heterologous proteins in the yeast Yarrowia lipolyticaQ74157870
OCH1 encodes a novel membrane bound mannosyltransferase: outer chain elongation of asparagine-linked oligosaccharidesQ27938727
Biosynthesis of lipid-linked oligosaccharides in yeast: the ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferaseQ27940341
Assembly of asparagine-linked oligosaccharidesQ28131690
The alpha- and beta-subunits are required for expression of catalytic activity in the hetero-dimeric glucosidase II complex from human liverQ28141802
The heterodimeric structure of glucosidase II is required for its activity, solubility, and localization in vivoQ28142779
Endoplasmic reticulum glucosidase II is composed of a catalytic subunit, conserved from yeast to mammals, and a tightly bound noncatalytic HDEL-containing subunitQ28296143
Quality control in the endoplasmic reticulumQ29619929
The accumulation of Man(6)GlcNAc(2)-PP-dolichol in the Saccharomyces cerevisiae Deltaalg9 mutant reveals a regulatory role for the Alg3p alpha1,3-Man middle-arm addition in downstream oligosaccharide-lipid and glycoprotein glycan processingQ30836843
Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichia pastorisQ31139066
N-glycan modification in Aspergillus speciesQ33310581
Characterization of an extracellular lipase encoded by LIP2 in Yarrowia lipolyticaQ33603182
Partial purification and characterization of the glucosidases involved in the processing of asparagine-linked oligosaccharidesQ34285261
Substrate specificities of rat liver microsomal glucosidases which process glycoproteins.Q34285322
Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review.Q35737904
Engineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycansQ35913310
The N-glycan glucosidase system in Trypanosoma bruceiQ35922860
Glucosidase II beta subunit modulates N-glycan trimming in fission yeasts and mammalsQ37327323
Engineering of glycosylation in yeast and other fungi: current state and perspectivesQ37768357
Genetic evidence for the heterodimeric structure of glucosidase II. The effect of disrupting the subunit-encoding genes on glycoprotein foldingQ38321171
Glycoengineering of the methylotrophic yeast Hansenula polymorpha for the production of glycoproteins with trimannosyl core N-glycan by blocking core oligosaccharide assembly.Q38501961
Sugar-binding activity of the MRH domain in the ER alpha-glucosidase II beta subunit is important for efficient glucose trimmingQ39822062
Physiology and genetics of the dimorphic fungus Yarrowia lipolyticaQ41484640
Processing of carbohydrate units of glycoproteins. Characterization of a thyroid glucosidaseQ41739511
Glycosylation of proteins by oligosaccharide-lipids. Studies on a thyroid enzyme involved in oligosaccharide transfer and the role of glucose in this reactionQ41739515
Engineering the yeast Yarrowia lipolytica for the production of therapeutic proteins homogeneously glycosylated with Man₈GlcNAc₂ and Man₅GlcNAc₂.Q42380751
The MRH domain suggests a shared ancestry for the mannose 6-phosphate receptors and other N-glycan-recognising proteinsQ42655325
Use of HDEL-tagged Trichoderma reesei mannosyl oligosaccharide 1,2-alpha-D-mannosidase for N-glycan engineering in Pichia pastorisQ43662722
Protein expression and secretion in the yeast Yarrowia lipolyticaQ44408101
Functional analysis of the ALG3 gene encoding the Dol-P-Man: Man5GlcNAc2-PP-Dol mannosyltransferase enzyme of P. pastorisQ44807390
Identification and characterisation of LIP7 and LIP8 genes encoding two extracellular triacylglycerol lipases in the yeast Yarrowia lipolytica.Q45262663
Genetic analysis of glucosidase II beta-subunit in trimming of high-mannose-type glycans.Q46036021
Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technologyQ46170507
Effects of macromolecular crowding on glycoprotein processing enzymesQ46801040
Glycome mapping on DNA sequencing equipmentQ49057061
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue6
P407language of work or nameEnglishQ1860
P921main subjectYarrowia lipolyticaQ5222203
P304page(s)e39976
P577publication date2012-06-29
P1433published inPLOS OneQ564954
P1476titleEngineering Yarrowia lipolytica to produce glycoproteins homogeneously modified with the universal Man3GlcNAc2 N-glycan core
P478volume7

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cites work (P2860)
Q64244102Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast
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Q37356816Effects of Rho1, a small GTPase on the production of recombinant glycoproteins in Saccharomyces cerevisiae
Q52632444Efficient homologous recombination with short length flanking fragments in Ku70 deficient Yarrowia lipolytica strains.
Q89250893Engineering Yarrowia lipolytica for Use in Biotechnological Applications: A Review of Major Achievements and Recent Innovations
Q39055068Engineering yeast for producing human glycoproteins: where are we now?
Q38684097Lysosomal enzyme replacement therapies: Historical development, clinical outcomes, and future perspectives
Q38659790N-glycoprotein macroheterogeneity: biological implications and proteomic characterization.
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Q57474501Synthetic biology tools for engineering Yarrowia lipolytica
Q38547311Using glyco-engineering to produce therapeutic proteins.
Q38462703Yarrowia lipolytica: recent achievements in heterologous protein expression and pathway engineering.
Q34620008Yarrowia lipolytica: safety assessment of an oleaginous yeast with a great industrial potential

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