A novel method of selective removal of human DNA improves PCR sensitivity for detection of Salmonella Typhi in blood samples

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A novel method of selective removal of human DNA improves PCR sensitivity for detection of Salmonella Typhi in blood samples is …
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scholarly articleQ13442814

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P6179Dimensions Publication ID1045096436
P356DOI10.1186/1471-2334-12-164
P932PMC publication ID3482578
P698PubMed publication ID22839649
P5875ResearchGate publication ID230575120

P50authorAndrew J. PollardQ64861148
P2093author name stringLiqing Zhou
P2860cites workNew methods for selective isolation of bacterial DNA from human clinical specimens.Q53393267
Preanalytic removal of human DNA eliminates false signals in general 16S rDNA PCR monitoring of bacterial pathogens in blood.Q54547368
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Rapid diagnosis of typhoid fever by PCR assay using one pair of primers from flagellin gene of Salmonella typhi.Q30821057
Evaluation of new-generation serologic tests for the diagnosis of typhoid fever: data from a community-based surveillance in Calcutta, IndiaQ31057455
A study of typhoid fever in five Asian countries: disease burden and implications for controlsQ33330938
Searching for the elusive typhoid diagnosticQ33537360
Quantitation of bacteria in blood of typhoid fever patients and relationship between counts and clinical features, transmissibility, and antibiotic resistanceQ33670230
Effect of Bile on the Action of Blood Against SalmonellaQ35175868
PCR method to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the blood of patients with clinical enteric feverQ36672996
The laboratory diagnosis of enteric fever.Q37595229
Detection of Salmonella typhi in the blood of patients with typhoid fever by polymerase chain reactionQ38532964
Typhoid fever: a review of its impact and diagnostic problems.Q39352095
Quantitation of bacteria in bone marrow from patients with typhoid fever: relationship between counts and clinical featuresQ39464233
One-step 2-minute test to detect typhoid-specific antibodies based on particle separation in tubes.Q39554799
Specimens and culture media for the laboratory diagnosis of typhoid fever.Q39568308
A fast and highly sensitive blood culture PCR method for clinical detection of Salmonella enterica serovar TyphiQ40360977
Further evaluation of a multiplex PCR for differentiation of Salmonella paratyphi A from other salmonellae.Q40413524
Multiplex PCR for differential diagnosis of emerging typhoidal pathogens directly from blood samplesQ40421747
Diagnosis of typhoid fever by polymerase chain reactionQ40435483
Diagnostic value of the Widal test in areas endemic for typhoid feverQ40874778
Selective isolation of bacterial DNA from human clinical specimens.Q44078686
Dot enzyme immunoassay (Typhidot) in diagnosis of typhoid fever in childrenQ50072926
Development of an ultra rapid and simple multiplex polymerase chain reaction technique for detection of Salmonella typhiQ50078277
P275copyright licenseCreative Commons Attribution 2.0 GenericQ19125117
P6216copyright statuscopyrightedQ50423863
P921main subjectSalmonella typhiQ22111431
P304page(s)164
P577publication date2012-07-27
P1433published inBMC Infectious DiseasesQ15759919
P1476titleA novel method of selective removal of human DNA improves PCR sensitivity for detection of Salmonella Typhi in blood samples
P478volume12

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cites work (P2860)
Q91957975Ascertaining the burden of invasive Salmonella disease in hospitalised febrile children aged under four years in Blantyre, Malawi
Q37693233Blood culture-PCR to optimise typhoid fever diagnosis after controlled human infection identifies frequent asymptomatic cases and evidence of primary bacteraemia
Q30749214CRISPR is an optimal target for the design of specific PCR assays for salmonella enterica serotypes Typhi and Paratyphi A.
Q40654661Comparison of microbial DNA enrichment tools for metagenomic whole genome sequencing
Q42321268Detection of Salmonella enterica in Meat in Less than 5 Hours by a Low-Cost and Noncomplex Sample Preparation Method
Q49959408Detection of trace amounts of target DNA from massive background of nucleic acids by using the LM-PCR-based preamplification method
Q35941251Development and Evaluation of a Blood Culture PCR Assay for Rapid Detection of Salmonella Paratyphi A in Clinical Samples
Q35748638Diagnostics for invasive Salmonella infections: Current challenges and future directions
Q28084595Epidemiology, Clinical Presentation, Laboratory Diagnosis, Antimicrobial Resistance, and Antimicrobial Management of Invasive Salmonella Infections
Q38953346Experimental metagenomics and ribosomal profiling of the human skin microbiome
Q64280688Human and Extracellular DNA Depletion for Metagenomic Analysis of Complex Clinical Infection Samples Yields Optimized Viable Microbiome Profiles
Q36709092Metagenomic analysis of bloodstream infections in patients with acute leukemia and therapy-induced neutropenia
Q35215679Nuclease-assisted suppression of human DNA background in sepsis
Q36205210Progress in the molecular diagnosis of Lyme disease
Q40045224Salivary Anti-50 kDa Antibodies as a Useful Biomarker for Diagnosis of Typhoid Fever
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Q57825786Typhoid Fever Diagnosis in Endemic Countries: A Clog in the Wheel of Progress?

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