A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs

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A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs is …
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scholarly articleQ13442814

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P6179Dimensions Publication ID1007137584
P356DOI10.1186/1472-6750-13-41
P932PMC publication ID3702409
P698PubMed publication ID23663692
P5875ResearchGate publication ID236689600

P50authorKetan MalhotraQ42596330
P2093author name stringArlene D Albert
Nathan N Alder
Anthony Watts
Ashley R Long
Catherine C O'Brien
Christine T Schwall
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Single-molecule fluorescence spectroscopy using phospholipid bilayer nanodiscsQ41870217
Monitoring shifts in the conformation equilibrium of the membrane protein cytochrome P450 reductase (POR) in nanodiscsQ42519476
Rhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F(0) during ATP synthesisQ44594994
Regulation of respiration in brain mitochondria and synaptosomes: restrictions of ADP diffusion in situ, roles of tubulin, and mitochondrial creatine kinaseQ46070091
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Effect of alterations in the amphipathic microenvironment on the conformational stability of bovine opsin. 1. Mechanism of solubilization of disk membranes by the nonionic detergent, octyl glucosideQ54001193
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Integral Membrane Proteins in Nanodiscs Can Be Studied by Solution NMR SpectroscopyQ57243814
On cytochrome c oxidase. I. The extinction coefficients of cytochrome a and cytochrome a3Q68562704
Dimer to monomer conversion of the cytochrome b6 f complex. Causes and consequencesQ73617888
A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMSQ76859027
Tetramethyl rhodamine methyl ester (TMRM) is suitable for cytofluorometric measurements of mitochondrial membrane potential in cells treated with digitoninQ77915120
Separation of yeast phospholipids using one-dimensional thin-layer chromatographyQ81395881
Detergent-free formation and physicochemical characterization of nanosized lipid-polymer complexes: LipodisqQ83803102
Lipid-protein interactionsQ84157392
Supercomplexes in the respiratory chains of yeast and mammalian mitochondriaQ24599994
Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organismsQ24673118
Arrangement of electron transport chain components in bovine mitochondrial supercomplex I1III2IV1Q27673724
The whole structure of the 13-subunit oxidized cytochrome c oxidase at 2.8 AQ27732721
Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondriaQ27935509
Blue native PAGEQ28296330
Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active formQ29619372
Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymerQ30157249
How membranes shape protein structure.Q30328356
Amphipols can support the activity of a membrane enzyme.Q30331883
Lipid bilayer composition influences small multidrug transportersQ33387030
Amphipols from A to Z.Q33890444
Solubilization of membranes by detergentsQ33911350
The ratio of oxidative phosphorylation complexes I-V in bovine heart mitochondria and the composition of respiratory chain supercomplexesQ33953875
Quantitative Analysis of Spontaneous Mitochondrial DepolarizationsQ34183689
Amphipols: polymers that keep membrane proteins soluble in aqueous solutionsQ34412679
Responsive hydrophobically associating polymers: a review of structure and propertiesQ34456759
Applications of phospholipid bilayer nanodiscs in the study of membranes and membrane proteins.Q34607664
Blue Native electrophoresis to study mitochondrial and other protein complexesQ34675865
Mitochondrial membrane potential probes and the proton gradient: a practical usage guideQ35047150
Bicelles in structure-function studies of membrane-associated proteinsQ35087061
Amphipols: polymeric surfactants for membrane biology research.Q35545388
Disease-related misassembly of membrane proteinsQ35771542
How lipids affect the activities of integral membrane proteinsQ35935383
Membrane proteins, lipids and detergents: not just a soap opera.Q35935390
Solubilization of membrane protein complexes for blue native PAGE.Q37198139
Lipid-protein interactions drive membrane protein topogenesis in accordance with the positive inside ruleQ37346899
Native electrophoretic techniques to identify protein-protein interactionsQ37616080
Amphipols, nanodiscs, and fluorinated surfactants: three nonconventional approaches to studying membrane proteins in aqueous solutionsQ37715989
P304page(s)41
P577publication date2013-05-11
P1433published inBMC BiotechnologyQ15753225
P1476titleA detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs
P478volume13

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