Simple method for Shiga toxin 2e purification by affinity chromatography via binding to the divinyl sulfone group

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Simple method for Shiga toxin 2e purification by affinity chromatography via binding to the divinyl sulfone group is …
instance of (P31):
scholarly articleQ13442814

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P819ADS bibcode2013PLoSO...883577A
P356DOI10.1371/JOURNAL.PONE.0083577
P932PMC publication ID3858352
P698PubMed publication ID24340102
P5875ResearchGate publication ID259337541

P2093author name stringTakao Tsuji
Hideyuki Arimitsu
Keiko Sasaki
Hiroe Kojima
Tadashi Yanaka
P2860cites workClinical application of Clostridium botulinum type A neurotoxin purified by a simple procedure for patients with urinary incontinence caused by refractory destrusor overactivityQ40095430
Purification and characterization of an Escherichia coli Shiga-like toxin II variantQ40154038
Quantitative microtiter cytotoxicity assay for Shigella toxin.Q40230921
Cloning and nucleotide sequence analysis of the genes determining verocytotoxin production in a porcine edema disease isolate of Escherichia coliQ42653384
Genetically modified Shiga toxin 2e (Stx2e) producing Escherichia coli is a vaccine candidate for porcine edema diseaseQ43654014
Lincomycin-induced over-expression of mature recombinant cholera toxin B subunit and the holotoxin in Escherichia coliQ45357221
Large-scale preparation of Shiga toxin 2 in Escherichia coli for toxoid vaccine antigen production.Q54323381
Simple method of purification of Escherichia coli heat-labile enterotoxin and cholera toxin using immobilized galactose.Q54644600
Construction of mutant genes for a non-toxic verotoxin 2 variant (VT2vp1) of Escherichia coli and characterization of purified mutant toxins.Q54645159
Reproduction of Edema Disease of Swine with Purified Shiga-like Toxin-II VariantQ54703471
Thiophilic adsorption of immunoglobulins--analysis of conditions optimal for selective immobilization and purificationQ68932921
Globotetraosylceramide is recognized by the pig edema disease toxinQ69517260
Thiophilic adsorption--a new method for protein fractionationQ70109252
Binding of avian ovomucoid to shiga-like toxin type 1 and its utilization for receptor analog affinity chromatographyQ73933165
Shiga-toxin-producing Escherichia coli and haemolytic uraemic syndromeQ33365488
Edema diseaseQ33856899
Globotriaosylceramide, Gb3, is an alternative functional receptor for Shiga-like toxin 2e.Q35396919
Vaccination with genetically modified Shiga-like toxin IIe prevents edema disease in swineQ35461166
Protection of piglets against Edema disease by maternal immunization with Stx2e toxoidQ35665444
Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swineQ36215407
The pathogenesis of edema disease in pigs. A reviewQ36303500
Alteration of the carbohydrate binding specificity of verotoxins from Gal alpha 1-4Gal to GalNAc beta 1-3Gal alpha 1-4Gal and vice versa by site-directed mutagenesis of the binding subunitQ36785336
An enzymatic mutant of Shiga-like toxin II variant is a vaccine candidate for edema disease of swineQ36957798
Two copies of Shiga-like toxin II-related genes common in enterohemorrhagic Escherichia coli strains are responsible for the antigenic heterogeneity of the O157:H- strain E32511Q36974749
Comparison of the glycolipid receptor specificities of Shiga-like toxin type II and Shiga-like toxin type II variantsQ36977977
One step high yield affinity purification of shiga-like toxin II variants and quantitation using enzyme linked immunosorbent assays.Q38323078
Purification of Shiga toxin by alpha-D-galactose-(1----4)-beta-D-galactose-(1----4)-beta-D-glucose-(1- ---) receptor ligand-based chromatographyQ38342831
Purification of Shiga-like toxin 1 by pigeon egg white glycoproteins immobilized on Sepharose gelsQ38360747
A new Shiga toxin 2 variant (Stx2f) from Escherichia coli isolated from pigeons.Q39485510
Identification of new verocytotoxin type 2 variant B-subunit genes in human and animal Escherichia coli isolatesQ39556186
Purification of fully activated Clostridium botulinum serotype B toxin for treatment of patients with dystoniaQ39730246
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P4510describes a project that usesaffinity chromatographyQ382799
P433issue12
P407language of work or nameEnglishQ1860
P304page(s)e83577
P577publication date2013-12-10
P1433published inPLOS OneQ564954
P1476titleSimple method for Shiga toxin 2e purification by affinity chromatography via binding to the divinyl sulfone group
P478volume8

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cites work (P2860)
Q88298494Affinity chromatography for vaccines manufacturing: Finally ready for prime time?
Q53144080Development of a simple and rapid diagnosis method for swine edema disease to specifically detect Stx2e protein by immunochromatographic test.
Q38927469Isolation of B subunit-specific monoclonal antibody clones that strongly neutralize the toxicity of Shiga toxin 2.

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