Sensitive multiplex PCR assay to differentiate Lyme spirochetes and emerging pathogens Anaplasma phagocytophilum and Babesia microti

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Sensitive multiplex PCR assay to differentiate Lyme spirochetes and emerging pathogens Anaplasma phagocytophilum and Babesia microti is …
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scholarly articleQ13442814

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P6179Dimensions Publication ID1028399423
P356DOI10.1186/1471-2180-13-295
P932PMC publication ID3890647
P698PubMed publication ID24359556
P5875ResearchGate publication ID259446626

P2093author name stringKamfai Chan
Salvatore A E Marras
Nikhat Parveen
P2860cites workDiagnosis of lyme borreliosisQ22242237
Human granulocytic anaplasmosis and Anaplasma phagocytophilumQ24611005
Sequencing of the smallest Apicomplexan genome from the human pathogen Babesia microtiQ24630337
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Current management of human granulocytic anaplasmosis, human monocytic ehrlichiosis and Ehrlichia ewingii ehrlichiosisQ24658293
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Homogeneous scoring of single-nucleotide polymorphisms: comparison of the 5'-nuclease TaqMan assay and Molecular Beacon probesQ28142186
A clear and present danger: tick-borne diseases in EuropeQ28267851
Direct molecular detection and genotyping of Borrelia burgdorferi from whole blood of patients with early Lyme diseaseQ28730068
Severe babesiosis in Long Island: review of 34 cases and their complicationsQ33336666
Babesiosis diagnosis and treatmentQ33348504
Clinical Presentation of 26 Anaplasma phagocytophilum-Seropositive Dogs Residing in an Endemic AreaQ33392600
Anaplasmosis in dogs: the relation of haematological, biochemical and clinical alterations to antibody titre and PCR confirmed infectionQ33392996
Differences and similarities between culture-confirmed human granulocytic anaplasmosis and early lyme diseaseQ33405204
Detection and quantification of Lyme spirochetes using sensitive and specific molecular beacon probesQ33411988
Coinfection with Babesia microti and Borrelia burgdorferi in a western Wisconsin residentQ33502239
Containment of bioaerosol infection risk by the Xpert MTB/RIF assay and its applicability to point-of-care settings.Q33663182
Anaplasma phagocytophilum APH_1387 is expressed throughout bacterial intracellular development and localizes to the pathogen-occupied vacuolar membraneQ33826005
Assessment of polymicrobial infections in ticks in New York stateQ33905015
Seroprevalence of antibodies that react with Anaplasma phagocytophila, the agent of human granulocytic ehrlichiosis, in different populations in Westchester County, New YorkQ33960957
Rapid and sensitive quantification of Borrelia burgdorferi-infected mouse tissues by continuous fluorescent monitoring of PCR.Q33974550
Endemic babesiosis in another eastern state: New JerseyQ33984412
Nuclease-resistant immunostimulatory phosphodiester CpG oligodeoxynucleotides as human Toll-like receptor 9 agonists.Q34029664
Powassan virus infection: case series and literature review from a single institutionQ34034176
Transfusion-associated babesiosis in the United States: a description of casesQ34213698
Infections and coinfections of questing Ixodes ricinus ticks by emerging zoonotic pathogens in Western SwitzerlandQ34241283
Diagnosis and management of Lyme disease.Q34298628
Efficiencies of fluorescence resonance energy transfer and contact-mediated quenching in oligonucleotide probesQ34330023
Transfusion-transmitted Babesia spp.: bull's-eye on Babesia microtiQ34491565
Human babesiosis.Q34596343
Microbiological and serological diagnosis of Lyme borreliosisQ34608068
Role of experience and context in learning to diagnose Lyme diseaseQ34846060
Improving the yield of blood cultures from patients with early Lyme diseaseQ35066710
Antisense technologies. Improvement through novel chemical modificationsQ35106663
Epidemiology and impact of coinfections acquired from Ixodes ticksQ35153339
Strategies for enhancing the immunostimulatory effects of CpG oligodeoxynucleotidesQ35775517
Comparison of five diagnostic modalities for direct detection of Borrelia burgdorferi in patients with early Lyme disease.Q36040985
Vertical transmission of Babesia microti, United StatesQ36147969
Prevalence and genetic characterization of Powassan virus strains infecting Ixodes scapularis in ConnecticutQ36450867
Clinical diagnosis and treatment of human granulocytotropic anaplasmosisQ36657627
Detection of Borrelia burgdorferi nucleic acids after antibiotic treatment does not confirm viabilityQ36667562
Multiplex MassTag-PCR for respiratory pathogens in pediatric nasopharyngeal washes negative by conventional diagnostic testing shows a high prevalence of viruses belonging to a newly recognized rhinovirus cladeQ37015811
Use of sloppy molecular beacon probes for identification of mycobacterial speciesQ37156889
Thermodynamic basis of the enhanced specificity of structured DNA probesQ37200381
Perpetuation of the agent of human granulocytic ehrlichiosis in a deer tick-rodent cycleQ37578634
Anaplasma phagocytophilum and Ehrlichia chaffeensis: subversive manipulators of host cellsQ37725654
Evaluation of real-time PCR targeting hbb gene for Borrelia species identificationQ37745646
Tired of Lyme borreliosis. Lyme borreliosis in the Netherlands.Q37858866
Babesia: a world emerging.Q38032685
Ticks and tick-borne diseases: a One Health perspectiveQ38035663
Identification of a candidate glycosaminoglycan-binding adhesin of the Lyme disease spirochete Borrelia burgdorferiQ38314563
Short PNA molecular beacons for real-time PCR allelic discrimination of single nucleotide polymorphismsQ38343067
Comparison of Western immunoblotting and the C6 Lyme antibody test for laboratory detection of Lyme diseaseQ39967080
Lyme disease and human granulocytic anaplasmosis coinfection: impact of case definition on coinfection rates and illness severityQ40272063
Prevalence of Lyme disease agents and several emerging pathogens in questing ticks from the German Baltic coastQ40328085
Questing ticks in suburban forest are infected by at least six tick-borne pathogensQ40340687
Human granulocytic anaplasmosisQ40411043
Using molecular beacons to detect single-nucleotide polymorphisms with real-time PCR.Q40659996
Human Babesia microti incidence and Ixodes scapularis distribution, Rhode Island, 1998-2004.Q41908543
A new real-time PCR assay for improved detection of the parasite Babesia microti.Q42135989
Summary of Notifiable Diseases - United States, 2011.Q42256641
Tick-borne encephalitis virus and louping-ill virus may co-circulate in Southern NorwayQ42287438
Detection of tick-borne pathogens by MassTag polymerase chain reactionQ42374776
Comparison of the reservoir competence of medium-sized mammals and Peromyscus leucopus for Anaplasma phagocytophilum in ConnecticutQ43216719
Enzootic Babesia microti in MaineQ43217634
Relationship between tick bites and the seroprevalence of Babesia microti and Anaplasma phagocytophila (previously Ehrlichia sp.) in blood donorsQ43740289
Increasing health burden of human babesiosis in endemic sites.Q44585434
Serological survey in persons occupationally exposed to tick-borne pathogens in cases of co-infections with Borrelia burgdorferi, Anaplasma phagocytophilum, Bartonella spp. and Babesia microti.Q44747426
Evaluation of a rapid test for the diagnosis of pneumococcal pneumonia.Q45036894
Potential effects of mixed infections in ticks on transmission dynamics of pathogens: comparative analysis of published recordsQ45172326
Development of real time PCR to detect Toxoplasma gondii and Borrelia burgdorferi infections in postal samplesQ45230976
Co-circulation of Emerging Tick-Borne Pathogens in Middle GermanyQ48062879
Seroprevalence of Babesia microti in blood donors from Babesia-endemic areas of the northeastern United States: 2000 through 2007.Q50595410
Transfusion-transmitted Babesia microti identified through hemovigilance.Q50602371
Design and optimization of molecular beacon real-time polymerase chain reaction assays.Q50794488
Lyme borreliosis in Sweden--diagnostic performance of five commercial Borrelia serology kits using sera from well-defined patient groups.Q51038614
Retracted: Established and emerging pathogens inIxodes ricinusticks collected from birds on a conservation island in the Baltic SeaQ51618868
Utility of a commercial immunoblot kit (BAG-Borrelia blot) in the diagnosis of the preliminary stages of Lyme disease.Q51826071
Preventing transfusion-transmitted babesiosis: preliminary experience of the first laboratory-based blood donor screening program.Q54317268
Transmission of Babesia microti in Minnesota through four blood donations from the same donor over a 6-month periodQ56776376
Performance of a commercially available in-clinic ELISA for the detection of antibodies againstAnaplasma phagocytophilum, Ehrlichia canis, andBorrelia burgdorferiandDirofilaria immitisantigen in dogsQ57438205
Transfusion-transmitted babesiosis: achieving successful mitigation while balancing cost and donor lossQ60423794
Experimental transmission of Ehrlichia chaffeensis (Rickettsiales: Ehrlichieae) among white-tailed deer by Amblyomma americanum (Acari: Ixodidae)Q71896222
Multicolor molecular beacons for allele discriminationQ74109331
Multiplex detection of single-nucleotide variations using molecular beaconsQ74618457
Lyme borreliosis: clinical case definitions for diagnosis and management in EuropeQ82783114
Summary of notifiable diseases: United States, 2009Q84088142
Seasonal prevalence of serum antibodies to whole cell and recombinant antigens of Borrelia burgdorferi and Anaplasma phagocytophilum in white-tailed deer in ConnecticutQ84676755
Quantitation of cell-associated borrelial DNA in the blood of Lyme disease patients with erythema migransQ84766435
P921main subjectLyme diseaseQ201989
Anaplasma phagocytophilumQ292189
Babesia microtiQ7777786
emerging pathogenQ108429945
P304page(s)295
P577publication date2013-12-20
P1433published inBMC MicrobiologyQ15759430
P1476titleSensitive multiplex PCR assay to differentiate Lyme spirochetes and emerging pathogens Anaplasma phagocytophilum and Babesia microti
P478volume13

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cites work (P2860)
Q36249774A novel quantitative PCR detects Babesia infection in patients not identified by currently available non-nucleic acid amplification tests
Q60909088Age-Related Differential Stimulation of Immune Response by and During Acute Phase of Infection Affects Disease Severity
Q49492331Babesia microti Infection Changes Host Spleen Architecture and Is Cleared by a Th1 Immune Response
Q47381281Borrelia burgdorferi glycosaminoglycan binding protein, Bgp in the B31 strain is not essential for infectivity despite facilitating adherence and tissue colonization
Q40419427Comparison of Babesia microti Real-Time Polymerase Chain Reaction Assays for Confirmatory Diagnosis of Babesiosis
Q50010299Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR.
Q40178121Detection and Differentiation of Lyme Spirochetes and Other Tick-Borne Pathogens from Blood Using Real-Time PCR with Molecular Beacons
Q40230739Detection of Anaplasma phagocytophilum, Babesia microti, Borrelia burgdorferi, Borrelia miyamotoi, and Powassan Virus in Ticks by a Multiplex Real-Time Reverse Transcription-PCR Assay
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Q55354078Efficient detection of symptomatic and asymptomatic patient samples for Babesia microti and Borrelia burgdorferi infection by multiplex qPCR.
Q40099254High-resolution melting PCR assay, applicable for diagnostics and screening studies, allowing detection and differentiation of several Babesia spp. infecting humans and animals
Q64116385KRAS genotyping by digital PCR combined with melting curve analysis
Q92239120Protozoan Parasite Babesia microti Subverts Adaptive Immunity and Enhances Lyme Disease Severity
Q34650391Relevance of chronic lyme disease to family medicine as a complex multidimensional chronic disease construct: a systematic review
Q41145227Utilization of a real-time PCR assay for diagnosis of Babesia microti infection in clinical practice
Q40734686Validate or falsify: Lessons learned from a microscopy method claimed to be useful for detecting Borrelia and Babesia organisms in human blood

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