Cholera holotoxin assembly requires a hydrophobic domain at the A-B5 interface: mutational analysis and development of an in vitro assembly system

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Cholera holotoxin assembly requires a hydrophobic domain at the A-B5 interface: mutational analysis and development of an in vitro assembly system is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1128/IAI.71.7.4093-4101.2003
P932PMC publication ID162025
P698PubMed publication ID12819100

P50authorWim G J HolQ114292749
P2093author name stringRandall K Holmes
Jarrod L Erbe
Juliette K Tinker
P2860cites workSimple method of purification of Escherichia coli heat-labile enterotoxin and cholera toxin using immobilized galactose.Q54644600
Dissociation and recombination of the subunits of the cholera enterotoxin (choleragen)Q67291725
Assembly of the B subunit pentamer of Escherichia coli heat-labile enterotoxin. Kinetics and molecular basis of rate-limiting steps in vitroQ71245693
Large-scale production of Vibrio cholerae toxin B subunit for use in oral vaccinesQ72271829
In vitro assembly of novel cholera toxin-like complexesQ73886892
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Structure-based discovery of a pore-binding ligand: towards assembly inhibitors for cholera and related AB5 toxinsQ77789698
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Refined structure of Escherichia coli heat-labile enterotoxin, a close relative of cholera toxinQ27732145
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A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genesQ29547324
RASMOL: biomolecular graphics for allQ29547757
Coordinated assembly of multisubunit proteins: oligomerization of bacterial enterotoxins in vivo and in vitroQ33650535
Membrane traffic and the cellular uptake of cholera toxinQ33681186
AB(5) toxins: structures and inhibitor designQ33927939
Biological and biochemical characterization of variant A subunits of cholera toxin constructed by site-directed mutagenesisQ33996430
Identification of motifs in cholera toxin A1 polypeptide that are required for its interaction with human ADP-ribosylation factor 6 in a bacterial two-hybrid systemQ35852634
In vitro formation of hybrid toxins between subunits of Escherichia coli heat-labile enterotoxin and those of cholera enterotoxinQ36430049
Fusion proteins containing the A2 domain of cholera toxin assemble with B polypeptides of cholera toxin to form immunoreactive and functional holotoxin-like chimerasQ36971660
Characterization of monoclonal antibodies that react with unique and cross-reacting determinants of cholera enterotoxin and its subunitsQ37100040
Intermolecular interactions between the A and B subunits of heat-labile enterotoxin from Escherichia coli promote holotoxin assembly and stability in vivoQ37352032
ADP-ribosylation of membrane proteins catalyzed by cholera toxin: basis of the activation of adenylate cyclaseQ37589612
Mutational analysis of ganglioside GM(1)-binding ability, pentamer formation, and epitopes of cholera toxin B (CTB) subunits and CTB/heat-labile enterotoxin B subunit chimeras.Q38291877
Structural basis for the differential toxicity of cholera toxin and Escherichia coli heat-labile enterotoxin. Construction of hybrid toxins identifies the A2-domain as the determinant of differential toxicityQ40975244
A kinetic model of intermediate formation during assembly of cholera toxin B-subunit pentamersQ43904675
Antibodies directed against the toxin-coregulated pilus isolated from Vibrio cholerae provide protection in the infant mouse experimental cholera modelQ45158043
An arabinose-inducible expression vector, pAR3, compatible with ColE1-derived plasmidsQ50143618
Construction and characterization of versatile cloning vectors for efficient delivery of native foreign proteins to the periplasm of Escherichia coli.Q54572886
P433issue7
P407language of work or nameEnglishQ1860
P921main subjectcholeraQ12090
mutational analysisQ1955810
hydrophobicityQ41854968
P304page(s)4093-4101
P577publication date2003-07-01
P1433published inInfection and ImmunityQ6029193
P1476titleCholera holotoxin assembly requires a hydrophobic domain at the A-B5 interface: mutational analysis and development of an in vitro assembly system
P478volume71

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cites work (P2860)
Q33788186Characterization of fluorescent chimeras of cholera toxin and Escherichia coli heat-labile enterotoxins produced by use of the twin arginine translocation system
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Q46379209Expression, immunogenicity and variation of iron-regulated surface protein A from bovine isolates of Staphylococcus aureus
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Q34565122Low cost tuberculosis vaccine antigens in capsules: expression in chloroplasts, bio-encapsulation, stability and functional evaluation in vitro
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Q41865473Structural inferences for Cholera toxin mutations in Vibrio cholerae
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