Comparison of flow cytometry, fluorescence microscopy and spectrofluorometry for analysis of gene electrotransfer efficiency

scientific article

Comparison of flow cytometry, fluorescence microscopy and spectrofluorometry for analysis of gene electrotransfer efficiency is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1007/S00232-014-9714-4
P698PubMed publication ID25146882
P5875ResearchGate publication ID264985777

P50authorDamijan MiklavčičQ12787232
P2093author name stringMojca Pavlin
Maša Kandušer
Igor Marjanovič
Mateja Manček Keber
P2860cites workGene transfer into mouse lyoma cells by electroporation in high electric fieldsQ24556501
A GFP-based reporter system to monitor nonsense-mediated mRNA decayQ24805903
The actin cytoskeleton has an active role in the electrotransfer of plasmid DNA in mammalian cellsQ30500347
Oligomerization of the human serotonin transporter and of the rat GABA transporter 1 visualized by fluorescence resonance energy transfer microscopy in living cells.Q31546930
Control by membrane order of voltage-induced permeabilization, loading and gene transfer in mammalian cellsQ32022289
Gene expression profiles in skeletal muscle after gene electrotransferQ33289258
Clinical applications of electrochemotherapyQ33904230
Quantitative model of small molecules uptake after in vitro cell electropermeabilizationQ33967478
Electrochemotherapy potentiation of antitumour effect of bleomycin by local electric pulsesQ34000544
Direct visualization at the single-cell level of electrically mediated gene deliveryQ34008999
Phase I trial of interleukin-12 plasmid electroporation in patients with metastatic melanomaQ34887442
High-efficiency gene transfer into skeletal muscle mediated by electric pulsesQ35123357
Electrotransfer as a non viral method of gene deliveryQ36737866
Antitumor effectiveness of electrochemotherapy: a systematic review and meta-analysisQ38043629
In vitro targeted gene electrotransfer to endothelial cells with plasmid DNA containing human endothelin-1 promoterQ39154948
Control by pulse parameters of electric field-mediated gene transfer in mammalian cellsQ39454885
Cell counting tool parameters optimization approach for electroporation efficiency determination of attached cells in phase contrast imagesQ39625817
Analysis and comparison of electrical pulse parameters for gene electrotransfer of two different cell linesQ39678108
Electropermeabilization of mammalian cells to macromolecules: control by pulse durationQ40128465
Gene electrotransfer of plasmid antiangiogenic metargidin peptide (AMEP) in disseminated melanoma: safety and efficacy results of a phase I first-in-man studyQ40236800
Electroporator with automatic change of electric field direction improves gene electrotransfer in-vitro.Q42425117
Effect of Mg ions on efficiency of gene electrotransfer and on cell electropermeabilizationQ43004419
Effect of different parameters used for in vitro gene electrotransfer on gene expression efficiency, cell viability and visualization of plasmid DNA at the membrane levelQ44939380
The role of electrophoresis in gene electrotransferQ45862244
Mechanisms involved in gene electrotransfer using high- and low-voltage pulses--an in vitro studyQ47288400
In vitro and in vivo electric field-mediated permeabilization, gene transfer, and expressionQ47664990
Effect of electric field vectoriality on electrically mediated gene delivery in mammalian cellsQ48558246
Analysis of cytotoxicity of melittin on adherent culture of human endothelial cells reveals advantage of fluorescence microscopy over flow cytometry and haemocytometer assayQ50236548
The role of electrically stimulated endocytosis in gene electrotransferQ50512623
Detection of protein interactions based on GFP fragment complementation by fluorescence microscopy and spectrofluorometry.Q50647410
Electro-mediated gene transfer and expression are controlled by the life-time of DNA/membrane complex formation.Q51776022
Quantification of cell hybridoma yields with confocal microscopy and flow cytometry.Q52004635
In vivo electroporation of skeletal muscle: threshold, efficacy and relation to electric field distribution.Q52207836
Permeability changes induced by electric impulses in vesicular membranesQ52959409
Theory of electroporation: A reviewQ55951425
Electrochemotherapy – An easy, highly effective and safe treatment of cutaneous and subcutaneous metastases: Results of ESOPE (European Standard Operating Procedures of Electrochemotherapy) studyQ56041031
In vivo gene electroinjection and expression in rat liverQ57018147
Importance of association between permeabilization and electrophoretic forces for intramuscular DNA electrotransferQ61445610
Electropermeabilization and fluorescent tracer exchange: the role of whole-cell capacitanceQ70953007
Electrochemotherapy, a new antitumor treatment. First clinical phase I-II trialQ71601162
Electropermeabilization of cell membranesQ73850437
P433issue12
P921main subjectflow cytometryQ1141429
P304page(s)1259-1267
P577publication date2014-08-22
P1433published inJournal of Membrane BiologyQ6295550
P1476titleComparison of flow cytometry, fluorescence microscopy and spectrofluorometry for analysis of gene electrotransfer efficiency
P478volume247

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cites work (P2860)
Q40811874Comparison of two automatic cell-counting solutions for fluorescent microscopic images
Q41682722Fundamental study on a gene transfection methodology for mammalian cells using water-in-oil droplet deformation in a DC electric field.
Q89879062Non-Viral in Vitro Gene Delivery: It is Now Time to Set the Bar!
Q47877224Quantification of green fluorescent protein-(GFP-) tagged membrane proteins by capillary gel electrophoresis.
Q50218513Structural behaviour and gene delivery in complexes formed between DNA and arginine-containing peptide amphiphiles

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