Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells

scientific article published on 2 June 2015

Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells is …
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scholarly articleQ13442814

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P6179Dimensions Publication ID1022253904
P356DOI10.1186/S12896-015-0145-9
P932PMC publication ID4450478
P698PubMed publication ID26033090
P5875ResearchGate publication ID277561518

P2093author name stringSay Kong Ng
Hing Kah Chin
Christine Lin Chin
Cara Sze Hui Chin
Ethan Tingfeng Lai
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A detailed understanding of the enhanced hypothermic productivity of interferon-gamma by Chinese-hamster ovary cellsQ40500220
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IRES bicistronic expression vectors for efficient creation of stable mammalian cell linesQ41145934
High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable markerQ41642360
Translational efficiency of EMCV IRES in bicistronic vectors is dependent upon IRES sequence and gene locationQ42598836
BAK and BAX deletion using zinc-finger nucleases yields apoptosis-resistant CHO cellsQ43272141
Decreasing lactate level and increasing antibody production in Chinese Hamster Ovary cells (CHO) by reducing the expression of lactate dehydrogenase and pyruvate dehydrogenase kinasesQ44735643
N-glycan analysis of human α1-antitrypsin produced in Chinese hamster ovary cellsQ45049201
Virus susceptibility of Chinese hamster ovary (CHO) cells and detection of viral contaminations by adventitious agent testingQ45375753
Effect of Akt overexpression on programmed cell death in antibody-producing Chinese hamster ovary cellsQ47254100
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Selection of high-producing CHO cells using NPT selection marker with reduced enzyme activity.Q51820133
Highly efficient selection of the stable clones expressing antibody-IL-2 fusion protein by a dicistronic expression vector containing a mutant neo gene.Q51820694
Selective charging of tRNA isoacceptors explains patterns of codon usage.Q54523461
Selection pressures on codon usage in the complete genome of bacteriophage T7Q72415262
Differential effect of exocytic SNAREs on the production of recombinant proteins in mammalian cellsQ83234334
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P304page(s)44
P577publication date2015-06-02
P1433published inBMC BiotechnologyQ15753225
P1476titleEngineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells
P478volume15

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cites work (P2860)
Q91286868A human expression system based on HEK293 for the stable production of recombinant erythropoietin
Q39299700Case study on human α1-antitrypsin: Recombinant protein titers obtained by commercial ELISA kits are inaccurate
Q90249925Glyco-engineered CHO cell lines producing alpha-1-antitrypsin and C1 esterase inhibitor with fully humanized N-glycosylation profiles
Q38701179New Mammalian Expression Systems
Q42064451Segmented linear modeling of CHO fed-batch culture and its application to large scale production.

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