A highly efficient, cell-free translation/translocation system prepared fromXenopuseggs

scientific article published on December 11, 1991

A highly efficient, cell-free translation/translocation system prepared fromXenopuseggs is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1093/NAR/19.23.6405
P953full work available at URLhttp://academic.oup.com/nar/article-pdf/19/23/6405/7075028/19-23-6405.pdf
https://europepmc.org/articles/PMC329185
https://europepmc.org/articles/PMC329185?pdf=render
P932PMC publication ID329185
P698PubMed publication ID1754376
P5875ResearchGate publication ID21402166

P2093author name stringG. Matthews
A. Colman
P2860cites workTranslocation of proteins across the endoplasmic reticulum. I. Signal recognition protein (SRP) binds to in-vitro-assembled polysomes synthesizing secretory proteinQ24681488
Assembly of asparagine-linked oligosaccharidesQ28131690
Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulumQ28275854
The biogenesis of lysosomesQ29617860
Developmental expression of the protein product of Vg1, a localized maternal mRNA in the frog Xenopus laevisQ33562558
p40MO15, a cdc2-related protein kinase involved in negative regulation of meiotic maturation of Xenopus oocytesQ33922124
A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptidesQ34172156
A role for the nuclear envelope in controlling DNA replication within the cell cycleQ34175944
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggsQ34190376
Cyclin synthesis drives the early embryonic cell cycleQ34667885
Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extractsQ34694399
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic componentsQ34711651
DNA ligase I from Xenopus laevis eggsQ35913730
Oligonucleotide site-directed mutagenesis in Xenopus egg extractsQ36065391
Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous componentsQ36204254
Expression of human cathepsin D in Xenopus oocytes: phosphorylation and intracellular targetingQ36217785
Site of addition of N-acetyl-galactosamine to the E1 glycoprotein of mouse hepatitis virus-A59Q36218544
Protein transport from endoplasmic reticulum to the Golgi complex can occur during meiotic metaphase in Xenopus oocytesQ36221570
Signal recognition particle mediates a transient elongation arrest of preprolactin in reticulocyte lysateQ36222313
Repair of UV-induced lesions in Xenopus laevis oocytesQ36925101
The spectrum of anionic oligosaccharides released by endo-beta-N-acetylglucosaminidase H from glycoproteins. Structural studies and interactions with the phosphomannosyl receptor.Q38354844
Mechanism of protein translocation across the endoplasmic reticulum membraneQ39507809
Regulation of protein export from the endoplasmic reticulumQ39544463
Cell-free synthesis and membrane insertion of mouse H-2Dd histocompatibility antigen and β2-microglobulinQ39566854
Assembly of SV40 chromatin in a cell-free system from Xenopus eggsQ40047973
Preparative synthesis of globin in a continuous cell-free translation system from rabbit reticulocytes.Q40450389
Binding to membrane proteins within the endoplasmic reticulum cannot explain the retention of the glucose-regulated protein GRP78 in Xenopus oocytesQ41091416
Protein translocation across wheat germ microsomal membranes requires an SRP-like componentQ41345594
Signal recognition particle (SRP) does not mediate a translational arrest of nascent secretory proteins in mammalian cell-free systemsQ41408691
Secretion in yeast: reconstitution of the translocation and glycosylation of alpha-factor and invertase in a homologous cell-free systemQ44301810
A developmentally regulated activity that unwinds RNA duplexesQ45204994
The secretory pathway is blocked between the trans-Golgi and the plasma membrane during meiotic maturation in Xenopus oocytesQ46519437
Preparation of microsomal membranes for cotranslational protein translocationQ49487241
Efficient cleavage and segregation of nascent presecretory proteins in a reticulocyte lysate supplemented with microsomal membranesQ49487721
Generation of a lysosomal enzyme targeting signal in the secretory protein pepsinogen.Q50804016
The control of DNA replication in a cell-free extract that recapitulates a basic cell cycle in vitro.Q52251088
Anti-ovalbumin monoclonal antibodies interact with their antigen in internal membranes of Xenopus oocytesQ61759009
Synthesis and secretion of mouse immunoglobulin chains from Xenopus oocytesQ61759011
An efficient cell-free translation system from Aspergillus nidulans and in vitro translocation of prepro-alpha-factor across Aspergillus microsomesQ62692098
Expression of coronavirus E1 and rotavirus VP10 membrane proteins from synthetic RNAQ69016094
Preparation and characterization of cell-free protein synthesis systems from oocytes and eggs of Xenopus laevisQ69452411
In vitro protein translocation across the yeast endoplasmic reticulum: ATP-dependent posttranslational translocation of the prepro-alpha-factorQ70137148
Protein synthesis during maturation promoting factor- and progesterone-induced maturation in Xenopus oocytesQ70202275
Substrate recognition by oligosaccharyl transferase. Inhibition of co-translational glycosylation by acceptor peptidesQ71240131
Quantitative changes in protein synthesis during oogenesis in Xenopus laevisQ93573325
P433issue23
P407language of work or nameEnglishQ1860
P921main subjectprotein biosynthesisQ211935
post-translational protein modificationQ898362
geneticsQ7162
cell-free systemQ1989580
P304page(s)6405-6412
P577publication date1991-12-01
1991-12-11
P1433published inNucleic Acids ResearchQ135122
P1476titleA highly efficient, cell-free translation/translocation system prepared from Xenopus eggs
A highly efficient, cell-free translation/translocation system prepared fromXenopuseggs
P478volume19

Reverse relations

cites work (P2860)
Q28344641A 250-nucleotide UA-rich element in the 3' untranslated region of Xenopus laevis Vg1 mRNA represses translation both in vivo and in vitro.
Q36693482A role for GPRx, a novel GPR3/6/12-related G-protein coupled receptor, in the maintenance of meiotic arrest in Xenopus laevis oocytes
Q39631353Activation of the kexin from Schizosaccharomyces pombe requires internal cleavage of its initially cleaved prosequence
Q33917446Association of prenylated proteins with the plasma membrane and the inner nuclear membrane is mediated by the same membrane-targeting motifs
Q36049558Content of intrinsic disorder influences the outcome of cell-free protein synthesis
Q33946685Cyclin B/cdc2 induces c-Mos stability by direct phosphorylation in Xenopus oocytes
Q47855261Differences in pH optima and calcium requirements for maturation of the prohormone convertases PC2 and PC3 indicates different intracellular locations for these events
Q42991629Differences in the autocatalytic cleavage of pro-PC2 and pro-PC3 can be attributed to sequences within the propeptide and Asp310 of pro-PC2.
Q40752538Effect of C-domain N-glycosylation and deletion on rat pancreatic alpha-amylase secretion and activity
Q74151887Efficient translocation and processing with Xenopus egg extracts of proteins synthesized in rabbit reticulocyte lysate
Q42354373Excluded-volume effects in living cells
Q39580168Functional interaction between the bovine papillomavirus virus type 1 replicative helicase E1 and cyclin E-Cdk2.
Q28367202Identification of the glycosylation sites utilized on the V1a vasopressin receptor and assessment of their role in receptor signalling and expression
Q48886407Immunofluorescence and biochemical techniques to detect nuclear localization of ciliary neurotrophic factor in glial cells
Q40867543Involvement of the membrane lipid bilayer in sorting prohormone convertase 2 into the regulated secretory pathway
Q37639868Isolation and characterization of krp, a dibasic endopeptidase required for cell viability in the fission yeast Schizosaccharomyces pombe.
Q33843693Preparation of a highly active cell-free translation system from immature Xenopus laevis oocytes
Q34133507Screening for small molecule inhibitors of embryonic pathways: sometimes you gotta crack a few eggs
Q34068785Secretion and mesoderm-inducing activity of the TGF-beta-related domain of Xenopus Vg1.
Q41113020Stringent thiol-mediated retention in B lymphocytes and Xenopus oocytes correlates with inefficient IgM polymerization
Q24684570The effect of macromolecular crowding on chaperonin-mediated protein folding
Q49043028Ubiquitination of full‐length cyclin

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