A single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli

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A single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.3390/TOXINS4070487
P932PMC publication ID3407889
P698PubMed publication ID22852065
P5875ResearchGate publication ID230593368

P2093author name stringRobert E Mandrell
Xiaohua He
Beatriz Quiñones
Stephanie McMahon
P2860cites workPurification and some properties of a Vero toxin from a human strain of Escherichia coli that is immunologically related to Shiga-like toxin II (VT2)Q57563891
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Novel cell-based method to detect Shiga toxin 2 from Escherichia coli O157:H7 and inhibitors of toxin activityQ39896145
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Verotoxins and their glycolipid receptors.Q40844228
Shiga toxin Stx2 is heat-stable and not inactivated by pasteurizationQ42637986
Validation of a cell-free translation assay for detecting shiga toxin 2 in bacterial cultureQ43925107
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Use of a Vero cell-based fluorescent assay to assess relative toxicities of Shiga toxin 2 subtypes from Escherichia coli.Q50520744
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Typing of verotoxins by DNA colony hybridization with poly- and oligonucleotide probes, a bead-enzyme-linked immunosorbent assay, and polymerase chain reaction.Q54596095
Activation of Shiga toxin type 2d (Stx2d) by elastase involves cleavage of the C-terminal two amino acids of the A2 peptide in the context of the appropriate B pentamerQ57563866
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Escherichia coli Shiga toxin.Q33334138
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Enterohaemorrhagic Escherichia coli in human medicineQ33368999
The Shiga toxin genotype rather than the amount of Shiga toxin or the cytotoxicity of Shiga toxin in vitro correlates with the appearance of the hemolytic uremic syndromeQ33376974
Analysis of collection of hemolytic uremic syndrome-associated enterohemorrhagic Escherichia coliQ33380625
Toxin genotypes and plasmid profiles as determinants of systemic sequelae in Escherichia coli O157:H7 infectionsQ33429817
Sequence of a variant Shiga-like toxin type-I operon of Escherichia coli O111:H-.Q33493707
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A monoclonal immunoglobulin G antibody directed against an immunodominant linear epitope on the ricin A chain confers systemic and mucosal immunity to ricinQ33557719
Comparison of the relative toxicities of Shiga-like toxins type I and type II for miceQ33605233
Shiga/verocytotoxins and Shiga/verotoxigenic Escherichia coli in animalsQ33657147
Stx2 subtyping of Shiga toxin-producing Escherichia coli isolated from cattle in France: detection of a new Stx2 subtype and correlation with additional virulence factorsQ33973300
The RNA N-glycosidase activity of ricin A-chain. The characteristics of the enzymatic activity of ricin A-chain with ribosomes and with rRNA.Q34172482
Shiga toxin subtypes display dramatic differences in potencyQ34739913
Identification of Escherichia coli O157 by using a novel colorimetric detection method with DNA microarraysQ35067355
Sensitive detection of Shiga Toxin 2 and some of its variants in environmental samples by a novel immuno-PCR assayQ35080788
Escherichia coli serotype O55:H7 diversity supports parallel acquisition of bacteriophage at Shiga toxin phage insertion sites during evolution of the O157:H7 lineageQ35867741
Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swineQ36215407
Identification and characterization of Shiga toxin type 2 variants in Escherichia coli isolates from animals, food, and humansQ36899078
Two copies of Shiga-like toxin II-related genes common in enterohemorrhagic Escherichia coli strains are responsible for the antigenic heterogeneity of the O157:H- strain E32511Q36974749
Affinity purification and characterization of Shiga-like toxin II and production of toxin-specific monoclonal antibodiesQ36997934
Surveillance for Shiga toxin-producing Escherichia coli, Michigan, 2001-2005.Q37302625
First-time isolation and characterization of a bacteriophage encoding the Shiga toxin 2c variant, which is globally spread in strains of Escherichia coli O157.Q37624022
Specificity of PCR and serological assays in the detection of Escherichia coli Shiga toxin subtypes.Q38628823
Isolation of a lysogenic bacteriophage carrying the stx(1(OX3)) gene, which is closely associated with Shiga toxin-producing Escherichia coli strains from sheep and humansQ39466924
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue7
P921main subjectEscherichia coliQ25419
P304page(s)487-504
P577publication date2012-06-25
P1433published inToxinsQ15724569
P1476titleA single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli
P478volume4

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cites work (P2860)
Q35908198A New Immunoassay for Detecting All Subtypes of Shiga Toxins Produced by Shiga Toxin-Producing E. coli in Ground Beef
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Q33734620New high-affinity monoclonal antibodies against Shiga toxin 1 facilitate the detection of hybrid Stx1/Stx2 in vivo
Q28488900Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2
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Q34426721Toxin instability and its role in toxin translocation from the endoplasmic reticulum to the cytosol
Q41508665Virulence characteristics of Shiga toxin-producing Escherichia coli from raw meats and clinical samples

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