Purification and characterization of a novel form of 20 alpha-hydroxysteroid dehydrogenase from Clostridium scindens

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Purification and characterization of a novel form of 20 alpha-hydroxysteroid dehydrogenase from Clostridium scindens is …
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scholarly articleQ13442814

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P356DOI10.1128/JB.171.6.2925-2932.1989
P932PMC publication ID209996
P698PubMed publication ID2722736

P2093author name stringP B Hylemon
A E Krafft
P2860cites workAdrenocortical steroid C-20-keto reductaseQ74566402
SEPARATION OF A PURIFIED ADRENAL 20-ALPHA-HYDROXY-STEROID DEHYDROGENASEQ76944188
20-ALPHA-HYDROXYSTEROID DEHYDROGENASE ACTIVITY A FUNCTION OF HUMAN PLACENTAL 17-BETA-HYDROXYSTEROID DEHYDROGENASEQ76957997
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[20beta-Hydroxysteroid dehydrogenase. 1. Culture of Streptomyces hydrogenans and induction of the enzyme.]Q78943945
The reduction in vitro of 17 alpha-hydroxypregnenolone (3 beta, 17 alpha-dihydroxy-delta 5-pregnen-20-one) by rabbit skeletal muscleQ79081382
Purification and properties of rat ovarian 20 alpha-hydroxysteroid dehydrogenaseQ79106946
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High resolution two-dimensional electrophoresis of proteinsQ24608190
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Q25938983
Induction and purification of alpha- and beta-hydroxysteroid dehydrogenasesQ28212332
Active-site directed inactivation of rat ovarian 20 alpha-hydroxysteroid dehydrogenaseQ28361622
Thymus metabolises progesterone—possible enzymatic marker for T lymphocytesQ34161973
Oxidative degradation of testosterone by adaptive enzymesQ34231176
Human placental 17 beta-estradiol dehydrogenase and 20 alpha-hydroxysteroid dehydrogenase. Two activities at a single enzyme active siteQ34273532
A new spectrophotometric assay for protein in cell extractsQ39167282
Sequence and structure of D-glyceraldehyde 3-phosphate dehydrogenase from Bacillus stearothermophilusQ40068130
Progesterone Metabolism in Normal Human Endometrium During the Menstrual Cycle and in Endometrial CarcinomaQ40343413
The primary structure of a glyceraldehyde-3-phosphate dehydrogenase gene from Saccharomyces cerevisiaeQ41107725
Homologous nucleotide sequences at the 5' termini of messenger RNAs synthesized from the yeast enolase and glyceraldehyde-3-phosphate dehydrogenase gene families. The primary structure of a third yeast glyceraldehyde-3-phosphate dehydrogenase gene.Q41604055
Separation of soluble mouse liver 21-desoxy-20-alpha-hydroxysteroid: NADP 20-alpha-oxidoreductase from 21-hydroxy-20-alpha-hydroxysteroid: NADP 20-alpha-oxidoreductase.Q43561225
Non-oligomeric nature of porcine testicular 20alpha-hydroxysteroid dehydrogenaseQ43756232
Studies of the human testis. IV. Testicular 20 alpha-hydroxysteroid dehydrogenase and steroid 17 alpha-hydroxylaseQ47836700
Cloning and sequencing of a deoxyribonucleic acid copy of glyceraldehyde-3-phosphate dehydrogenase messenger ribonucleic acid isolated from chicken muscle.Q48399275
Structural comparison of two nontandemly repeated yeast glyceraldehyde-3-phosphate dehydrogenase genesQ48413953
Biosynthesis of androgen from cortisol by a species of Clostridium recovered from human fecal floraQ48685239
Pyridine-nucleotide transhydrogenase. 5. Kinetic studies on transhydrogenase from Azotobacter vinelandii.Q53933461
20 -hydroxysteroid dehydrogenase of porcine testes.Q54506749
The glyceraldehyde 3-phosphate dehydrogenases of liver and muscle. Cooperative interactions and conditions for functional reversibility.Q54728509
Glyceraldehyde 3-Phosphate Dehydrogenase from Pig MuscleQ59056349
Amino-acid Sequence of Glyceraldehyde 3-Phosphate Dehydrogenase from Lobster MuscleQ59076368
Isolation and enzymatic properties of the mitochondrial reduced diphosphopyridine nucleotide dehydrogenaseQ68594866
Microsomal 20 alpha-hydroxysteroid dehydrogenase activity for progesterone in human placentaQ68737375
Cofactor requirements of steroid-17-20-desmolase and 20 alpha-hydroxysteroid dehydrogenase activities in cell extracts of Clostridium scindensQ68767888
Expression of 20 alpha-hydroxysteroid dehydrogenase activity in human lymphoid and non lymphoid cellsQ68795192
17 beta-hydroxysteroid and 20 alpha-hydroxysteroid dehydrogenase activities of human placental microsomes: kinetic evidence for two enzymes differing in substrate specificityQ69839296
Purification and characterization of 20 alpha-hydroxysteroid dehydrogenase from bull testisQ69854846
Mode of action of steroid desmolase and reductases synthesized by Clostridium "scindens" (formerly Clostridium strain 19)Q70715630
Kinetic studies of rat ovarian 20 alpha-hydroxysteroid dehydrogenaseQ70939659
D-glyceraldehyde-3-phosphate dehydrogenase. Amino-acid sequence of the enzyme from the extreme thermophile Thermus aquaticusQ71515016
Estradiol 17 beta-dehydrogenase and 20 alpha-hydroxysteroid dehydrogenase from human placental cytosol: one enzyme with two activities?Q71991515
Purification of 20 alpha-hydroxysteroid oxidoreductase from bovine fetal erythrocytesQ72012936
Partial characterization of the cytoplasmic 20 alpha-hydroxysteroid dehydrogenase (EC 1.1.1.149) of the human placenta at termQ72046792
Studies on mouse liver 21-desoxy- and 21-hydroxy-20α-hydroxysteroid : NADP 20α-oxidoreductaseQ72128431
On the molecular specificity of steroid-enzyme combinations; the kinetics of beta-hydroxysteroid dehydrogenaseQ73802928
The C-20alpha reduction of steroids by hog liver preparationsQ74008569
Substrate specificity of enzymes reducing the 11- and 20-keto groups of steroidsQ74091651
P433issue6
P407language of work or nameEnglishQ1860
P921main subjecthydroxysteroidQ2157080
Clostridium scindensQ3681149
P304page(s)2925-2932
P577publication date1989-06-01
P1433published inJournal of BacteriologyQ478419
P1476titlePurification and characterization of a novel form of 20 alpha-hydroxysteroid dehydrogenase from Clostridium scindens
P478volume171

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cites work (P2860)
Q35967895Characterization and regulation of the NADP-linked 7 alpha-hydroxysteroid dehydrogenase gene from Clostridium sordellii
Q30416438Clostridium scindens: a human gut microbe with a high potential to convert glucocorticoids into androgens
Q54295444Escherichia coli kduD encodes an oxidoreductase that converts both sugar and steroid substrates.
Q42290255Identification and characterization of a 20β-HSDH from the anaerobic gut bacterium Butyricicoccus desmolans ATCC 43058.
Q37731880Lipid metabolism in anaerobic ecosystems
Q28369669Molecular cloning of cDNA for rat ovarian 20 alpha-hydroxysteroid dehydrogenase (HSD1)
Q34347668Purification and characterization of a novel dimeric 20 alpha-hydroxysteroid dehydrogenase from Tetrahymena pyriformis
Q64985381The desA and desB genes from Clostridium scindens ATCC 35704 encode steroid-17,20-desmolase.

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