Spindle microtubule dynamics in sea urchin embryos: analysis using a fluorescein-labeled tubulin and measurements of fluorescence redistribution after laser photobleaching

scientific article

Spindle microtubule dynamics in sea urchin embryos: analysis using a fluorescein-labeled tubulin and measurements of fluorescence redistribution after laser photobleaching is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1083/JCB.99.6.2165
P932PMC publication ID2113564
P698PubMed publication ID6501418

P2093author name stringM L Karow
E D Salmon
J R McIntosh
W M Saxton
R J Leslie
P2860cites workMobility measurement by analysis of fluorescence photobleaching recovery kineticsQ24536888
Implications of treadmilling for the stability and polarity of actin and tubulin polymers in vivoQ24681944
Microtubular origin of mitotic spindle form birefringence. Demonstration of the applicability of Wiener's equationQ31114481
Spindle microtubules: thermodynamics of in vivo assembly and role in chromosome movementQ34019832
Dynamics of mitotic spindle organization and functionQ34060702
The Formation, Structure, and Composition of the Mammalian Kinetochore and Kinetochore FiberQ34249698
Fluorescence photobleaching recovery in solutions of labeled actin.Q34252381
Functional organization of mitotic microtubules. Physical chemistry of the in vivo equilibrium systemQ34271128
Diffusion of injected macromolecules within the cytoplasm of living cellsQ35403864
Experimental manipulation of the amount of tubulin available for assembly into the spindle of dividing sea urchin eggsQ36198374
Distribution of fluorescently labeled actin in living sea urchin eggs during early developmentQ36200600
Distribution of tubulin-containing structures in the egg of the sea urchin Strongylocentrotus purpuratus from fertilization through first cleavageQ36201222
Calcium-labile mitotic spindles isolated from sea urchin eggs (Lytechinus variegatus).Q36203663
Decoration of spindle microtubules with Dynein: evidence for uniform polarityQ36204384
Direct visualization of fluorescein-labeled microtubules in vitro and in microinjected fibroblastsQ36204458
Quantitative studies on the polarization optical properties of living cells II. The role of microtubules in birefringence of the spindle of the sea urchin eggQ36204771
Ultraviolet microbeam irradiations of mitotic diatoms: investigation of spindle elongationQ36206785
Direct electron microscopic visualization of barbed end capping and filament cutting by intestinal microvillar 95-kdalton protein (villin): a new actin assembly assay using the Limulus acrosomal processQ36206873
Microinjection of fluorescent tubulin into dividing sea urchin cellsQ36208072
Rapid rate of tubulin dissociation from microtubules in the mitotic spindle in vivo measured by blocking polymerization with colchicineQ36211372
Diffusion coefficient of fluorescein-labeled tubulin in the cytoplasm of embryonic cells of a sea urchin: video image analysis of fluorescence redistribution after photobleachingQ36211987
Assembly properties of fluorescein-labeled tubulin in vitro before and after fluorescence bleachingQ36212004
Tubulin dynamics in cultured mammalian cellsQ36212188
Interference of GTP hydrolysis in the mechanism of microtubule assembly: an experimental studyQ36253759
Mobility of cytoplasmic and membrane-associated actin in living cells.Q36306376
Cell motility by labile association of molecules. The nature of mitotic spindle fibers and their role in chromosome movementQ36427621
Measurement of the lateral mobility of cell surface components in single, living cells by fluorescence recovery after photobleachingQ36731542
Bioenergetics and kinetics of microtubule and actin filament assembly-disassemblyQ40088525
Microinjection of fluorescently labeled proteins into living cells with emphasis on cytoskeletal proteinsQ40265470
Microtubule treadmills--possible molecular machineryQ40316875
Mitotic mechanism based on intrinsic microtubule behaviourQ45160160
A kinetic analysis of assembly-disassembly at opposite microtubule ends.Q48964676
Incorporation of radioactive tubulin into microtubules at steady state. Experimental and theoretical analyses of diffusional and directional flux.Q52754622
International workshop on the application of fluorescence photobleaching techniques to problems in cell biology.Q53435055
The role of membranes in the organization of the mitotic apparatusQ67428145
Fluorescent analog cytochemistry of contractile proteinsQ70680123
Fluorescent localization of contractile proteins in tissue culture cellsQ71440949
Mobility of microinjected rhodamine actin within living chicken gizzard cells determined by fluorescence photobleaching recoveryQ71843966
P433issue6
P407language of work or nameEnglishQ1860
P921main subjectlaserQ38867
sea urchinQ83483
P304page(s)2165-2174
P577publication date1984-12-01
P1433published inJournal of Cell BiologyQ1524550
P1476titleSpindle microtubule dynamics in sea urchin embryos: analysis using a fluorescein-labeled tubulin and measurements of fluorescence redistribution after laser photobleaching
P478volume99

Reverse relations

cites work (P2860)
Q4170668942 Analyzing dynamic properties of intermediate filaments
Q28074607A Brief History of Research on Mitotic Mechanisms
Q41668719A method improving the accuracy of fluorescence recovery after photobleaching analysis.
Q33390368An experimental and computational study of effects of microtubule stabilization on T-cell polarity
Q41526786An investigation of microtubule organization and functions in living Drosophila embryos by injection of a fluorescently labeled antibody against tyrosinated alpha-tubulin
Q27309143An organelle-exclusion envelope assists mitosis and underlies distinct molecular crowding in the spindle region
Q36214587Analysis of the treadmilling model during metaphase of mitosis using fluorescence redistribution after photobleaching
Q28078705Anaphase B
Q36235181Antibodies to the kinesin motor domain and CENP-E inhibit microtubule depolymerization-dependent motion of chromosomes in vitro
Q36220502Assembly and exchange of intermediate filament proteins of neurons: neurofilaments are dynamic structures
Q36217906Assembly and turnover of detyrosinated tubulin in vivo
Q70119694Assembly of microtubules from nucleotide-depleted tubulin
Q36212004Assembly properties of fluorescein-labeled tubulin in vitro before and after fluorescence bleaching
Q39512555Assembly, disassembly, and exchange of glial fibrillary acidic protein
Q40344463Association of adenovirus with the microtubule organizing center
Q36625777Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle
Q37078593Basal body components exhibit differential protein dynamics during nascent basal body assembly
Q24651410Binding of E-MAP-115 to microtubules is regulated by cell cycle-dependent phosphorylation
Q45005782Biophysical chemical aspects of cellular cryobehavior
Q36221845Biotin-tubulin incorporates into kinetochore fiber microtubules during early but not late anaphase
Q33224616CLAMP, a novel microtubule-associated protein with EB-type calponin homology
Q36220210Cell cycle-dependent changes in the dynamics of MAP 2 and MAP 4 in cultured cells
Q36233793Centrin plays an essential role in microtubule severing during flagellar excision in Chlamydomonas reinhardtii
Q38651018Characteristics of microtubules at the different stages of neuronal differentiation and maturation.
Q36225263Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends
Q36217297Ciliary protein conservation during development in the ciliated protozoan, Oxytricha
Q34888171Connexin 43 connexon to gap junction transition is regulated by zonula occludens-1.
Q41678512Coupling cell division and cell death to microtubule dynamics
Q37452572Crosstalk between microtubule attachment complexes ensures accurate chromosome segregation
Q24672791Cyclin B interaction with microtubule-associated protein 4 (MAP4) targets p34cdc2 kinase to microtubules and is a potential regulator of M-phase microtubule dynamics
Q70220226Detection of single fluorescent microtubules and methods for determining their dynamics in living cells
Q39320845Differential functional interplay of TOGp/XMAP215 and the KinI kinesin MCAK during interphase and mitosis
Q36213842Direct observation of microtubule treadmilling by electron microscopy
Q36214526Distribution of tyrosinated and nontyrosinated alpha-tubulin during mitosis
Q33968956Dosage suppressors of a benomyl-dependent tubulin mutant: evidence for a link between microtubule stability and cellular metabolism
Q36462439Dynamics of microtubule depolymerization in monocytes
Q44975135Effect of microinjected calcium-calmodulin on mitosis in PtK2 cells
Q42828716Effects of 50 Hz magnetic fields on gap junctional intercellular communication in NIH3T3 cells.
Q34291152Efficiency of organelle capture by microtubules as a function of centrosome nucleation capacity: general theory and the special case of polyspermia
Q35575300Endocytosis of adenovirus and adenovirus capsid proteins
Q24680491Exchange of actin subunits at the leading edge of living fibroblasts: possible role of treadmilling
Q32055070Exchangeability of actin in cardiac myocytes and fibroblasts as determined by fluorescence photobleaching recovery
Q36214022Exchangeability of alpha-actinin in living cardiac fibroblasts and muscle cells
Q30492820Fast microtubule dynamics in meiotic spindles measured by single molecule imaging: evidence that the spindle environment does not stabilize microtubules
Q34088131Flow rate measurements in isolated perfused kidney tubules by fluorescence photobleaching recovery
Q36219520Fluorescent microtubules break up under illumination
Q34453605Force generation by microtubule assembly/disassembly in mitosis and related movements
Q42822935Gamma-tubulin is a centrosomal protein required for cell cycle-dependent microtubule nucleation
Q93086464High-resolution imaging reveals how the spindle midzone impacts chromosome movement
Q37376053How the transition frequencies of microtubule dynamic instability (nucleation, catastrophe, and rescue) regulate microtubule dynamics in interphase and mitosis: analysis using a Monte Carlo computer simulation
Q43410846Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules
Q59082527Identification of kinesin in sea urchin eggs and evidence for its localization in the mitotic spindle
Q64375127Impact of dynamin 2 on adenovirus nuclear entry
Q30461866In vitro reactivation of anaphase B in isolated spindles of the sea urchin egg.
Q48401524Incorporation and turnover of labeled exogenous tubulin in the mitotic spindles of Chaetopterus oocytes and HeLa cells
Q68090522Incorporation of fluorescently labeled contractile proteins into freshly isolated living adult cardiac myocytes
Q34630610Insights into the micromechanical properties of the metaphase spindle
Q36217377Interzone microtubule behavior in late anaphase and telophase spindles
Q34191608Kinesin-1 and dynein at the nuclear envelope mediate the bidirectional migrations of nuclei
Q36236166Kinetochore microtubule dynamics and the metaphase-anaphase transition
Q36222387Kinetochores are transported poleward along a single astral microtubule during chromosome attachment to the spindle in newt lung cells
Q36219491Laser-transected microtubules exhibit individuality of regrowth, however most free new ends of the microtubules are stable
Q24680571Listeria monocytogenes exploits normal host cell processes to spread from cell to cell
Q42015218Mal3, the fission yeast homologue of the human APC-interacting protein EB-1 is required for microtubule integrity and the maintenance of cell form
Q38682090Mechanism of Action of the Anti-Inflammatory Connexin43-Mimetic Peptide JM2.
Q37242379Mechanisms of Mitotic Spindle Assembly
Q35746819Medicinal Plants: A Potential Source of Compounds for Targeting Cell Division.
Q37728181Metaphase Spindle Assembly
Q37621924Micromechanics of the vertebrate meiotic spindle examined by stretching along the pole-to-pole axis
Q39696100Microscopic imaging of cells
Q41128629Microtubule dissassembly in vivo: intercalary destabilization and breakdown of microtubules in the heliozoan Actinocoryne contractilis
Q36218473Microtubule dynamics and chromosome motion visualized in living anaphase cells
Q33598778Microtubule dynamics in Xenopus egg extracts
Q36214474Microtubule dynamics in interphase cells
Q31019125Microtubule dynamics in living dividing plant cells: confocal imaging of microinjected fluorescent brain tubulin
Q41555979Microtubule dynamics in nerve cells: analysis using microinjection of biotinylated tubulin into PC12 cells
Q67950302Microtubule dynamics in the chromosomal spindle fiber: analysis by fluorescence and high-resolution polarization microscopy
Q69521265Microtubule dynamics in the spindle. Theoretical aspects of assembly/disassembly reactions in vivo
Q36216411Microtubule dynamics in vivo: a test of mechanisms of turnover
Q41566744Microtubule dynamics investigated by microinjection of Paramecium axonemal tubulin: lack of nucleation but proximal assembly of microtubules at the kinetochore during prometaphase
Q40365906Microtubule flux in mitosis is independent of chromosomes, centrosomes, and antiparallel microtubules
Q34451728Microtubule stability in budding yeast: characterization and dosage suppression of a benomyl-dependent tubulin mutant
Q35633089Microtubule-severing activity in M phase.
Q30483931Microtubules and microscopes: how the development of light microscopic imaging technologies has contributed to discoveries about microtubule dynamics in living cells
Q40954603Microtubules and microtubule motors: mechanisms of regulation.
Q34303771Microtubules are stabilized in confluent epithelial cells but not in fibroblasts
Q39724370Microtubules get dynamic
Q30454177Microtubules in the metaphase-arrested mouse oocyte turn over rapidly
Q30442307Microtubules of the kinetochore fiber turn over in metaphase but not in anaphase
Q34434485Microtubules orient the mitotic spindle in yeast through dynein-dependent interactions with the cell cortex.
Q94069691Microtubules turn over rapidly
Q34200075Microtubules, chromosome movement, and reorientation after chromosomes are detached from the spindle by micromanipulation
Q35474798Mitosis futures: the past is prologue
Q34123562Mitosis: a history of division
Q40883514Mitosis: spindle assembly and chromosome motion
Q37381147Mitotic motors
Q36529300Mitotic spindle assembly by two different pathways in vitro
Q35695904Mos oncogene product associates with kinetochores in mammalian somatic cells and disrupts mitotic progression
Q36844040Mutations at phosphorylation sites of Xenopus microtubule-associated protein 4 affect its microtubule-binding ability and chromosome movement during mitosis
Q27940249Myosin-V is activated by binding secretory cargo and released in coordination with Rab/exocyst function
Q37378781Nonneuronal isoforms of STOP protein are responsible for microtubule cold stability in mammalian fibroblasts
Q36532315Okadaic acid induces interphase to mitotic-like microtubule dynamic instability by inactivating rescue
Q59067304Organization of microtubules in dendrites and axons is determined by a short hydrophobic zipper in microtubule-associated proteins MAP2 and tau
Q36213174Oscillatory movements of monooriented chromosomes and their position relative to the spindle pole result from the ejection properties of the aster and half-spindle
Q30503885Paxillin-dependent stimulation of microtubule catastrophes at focal adhesion sites
Q73878381Photoactivation of green fluorescent protein
Q36532077Poleward kinetochore fiber movement occurs during both metaphase and anaphase-A in newt lung cell mitosis
Q36529365Poleward microtubule flux mitotic spindles assembled in vitro
Q41583375Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence
Q28504095Positive control of cell division: FtsZ is recruited by SsgB during sporulation of Streptomyces
Q45259418Preparation and characterization of caged fluorescein tubulin
Q69900875Primary cilia cycle in PtK1 cells: effects of colcemid and taxol on cilia formation and resorption
Q54373868Probing the mechanisms of mitosis with Dictyostelium discoideum.
Q36212827Properties of the kinetochore in vitro. II. Microtubule capture and ATP-dependent translocation
Q30480485Quantitative analysis of an anaphase B switch: predicted role for a microtubule catastrophe gradient
Q37751257Quantitative characterization of filament dynamics by single-molecule lifetime measurements
Q34016870Rapid assembly dynamics of the Escherichia coli FtsZ-ring demonstrated by fluorescence recovery after photobleaching
Q30450922Reactivation of isolated mitotic apparatus: metaphase versus anaphase spindles
Q28760164Real-time observations of microtubule dynamic instability in living cells
Q33938476Regulation of a major microtubule-associated protein by MPF and MAP kinase
Q27311999Role of the Number of Microtubules in Chromosome Segregation during Cell Division
Q41069511Sea urchin microtubules
Q41439818Selectivity of methyl mercury effects on cytoskeleton and mitotic progression in cultured cells
Q39539389Shogaols at proapoptotic concentrations induce G(2)/M arrest and aberrant mitotic cell death associated with tubulin aggregation
Q34416537Simplified equation to extract diffusion coefficients from confocal FRAP data
Q33397362Spatial Fourier analysis of video photobleaching measurements. Principles and optimization
Q41791570Spindle microtubule dynamics: modulation by metabolic inhibitors
Q36217301Subunit exchange between smooth muscle myosin filaments
Q34333599Synchronous oscillations in microtubule polymerization
Q42338343Ted Salmon: kinetochores at the core of it all. Interview by Caitlin Sedwick
Q24645077The 3Ms of central spindle assembly: microtubules, motors and MAPs
Q30499485The Drosophila kinesin-like protein KLP67A is essential for mitotic and male meiotic spindle assembly
Q34172806The centrosome and its role in the organization of microtubules
Q69849432The cytotoxicity of chrysotile asbestos fibers to pulmonary alveolar macrophages. I. Effects of inhibitors of ADP-ribosyl transferase
Q30549670The differential roles of budding yeast Tem1p, Cdc15p, and Bub2p protein dynamics in mitotic exit
Q69784561The distribution of intermicrotubular bridges in meiotic spindles of the crane fly
Q34827544The dynamics of connexin expression, degradation and localisation are regulated by gonadotropins during the early stages of in vitro maturation of swine oocytes
Q70249152The effects of methylmercury on the cytoskeleton of murine embryonal carcinoma cells
Q33208041The forces that position a mitotic spindle asymmetrically are tethered until after the time of spindle assembly
Q36235584The impact of chromosomes and centrosomes on spindle assembly as observed in living cells
Q33902928The microtubule-destabilizing kinesin XKCM1 regulates microtubule dynamic instability in cells
Q46133042The minimum GTP cap required to stabilize microtubules
Q34123567The spindle: a dynamic assembly of microtubules and motors
Q48398790The stabilization of microtubules in isolated spindles by tubulin-colchicine complex
Q36361680Theoretical model of mitotic spindle microtubule growth for FRAP curve interpretation
Q36212188Tubulin dynamics in cultured mammalian cells
Q37943402Tubulin flux in the mitotic spindle: where does it come from, where is it going?
Q42762803Two Drosophila beta tubulin isoforms are not functionally equivalent
Q36215747Ultrastructural colocalization of tyrosinated and detyrosinated alpha-tubulin in interphase and mitotic cells
Q34138127Using micromanipulation to analyze control of vertebrate meiotic spindle size
Q34553174Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p
Q45290111Visualization of myosin exchange between synthetic thick filaments
Q59067947Visualization of the dynamic instability of individual microtubules by dark-field microscopy
Q36789718XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly
Q34134262XMAP215: a key component of the dynamic microtubule cytoskeleton

Search more.