A cloned Erwinia chrysanthemi Hrp (type III protein secretion) system functions in Escherichia coli to deliver Pseudomonas syringae Avr signals to plant cells and to secrete Avr proteins in culture

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A cloned Erwinia chrysanthemi Hrp (type III protein secretion) system functions in Escherichia coli to deliver Pseudomonas syringae Avr signals to plant cells and to secrete Avr proteins in culture is …
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scholarly articleQ13442814

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P819ADS bibcode1998PNAS...9510206H
P356DOI10.1073/PNAS.95.17.10206
P932PMC publication ID21486
P698PubMed publication ID9707625
P5875ResearchGate publication ID13576938

P2093author name stringCollmer A
Fouts DE
Ham JH
Bauer DW
P2860cites workEvidence that the Pseudomonas syringae pv. syringae hrp-linked hrmA gene encodes an Avr-like protein that acts in an hrp-dependent manner within tobacco cellsQ42659352
Molecular Basis of Gene-for-Gene Specificity in Bacterial Speck Disease of TomatoQ43463829
Erwinia chrysanthemi harpinEch: an elicitor of the hypersensitive response that contributes to soft-rot pathogenesis.Q43818903
Harpin, elicitor of the hypersensitive response produced by the plant pathogen Erwinia amylovoraQ47932082
The hrpC and hrpN operons of Erwinia chrysanthemi EC16 are flanked by plcA and homologs of hemolysin/adhesin genes and accompanying activator/transporter genesQ48013223
Characterization of avrE from Pseudomonas syringae pv. tomato: a hrp-linked avirulence locus consisting of at least two transcriptional units.Q48076249
Pseudomonas syringae pv. syringae harpinPss: a protein that is secreted via the Hrp pathway and elicits the hypersensitive response in plantsQ48111472
Unified nomenclature for broadly conserved hrp genes of phytopathogenic bacteria.Q48786134
Initiation of Plant Disease Resistance by Physical Interaction of AvrPto and Pto KinaseQ52522892
Expression of avrPphB, an avirulence gene from Pseudomonas syringae pv. phaseolicola, and the delivery of signals causing the hypersensitive reaction in bean.Q54570214
Expression of the Pseudomonas syringae avirulence protein AvrB in plant cells alleviates its dependence on the hypersensitive response and pathogenicity (Hrp) secretion system in eliciting genotype-specific hypersensitive cell death.Q54586263
Phenotypic expression of Pseudomonas syringae avr genes in E. coli is linked to the activities of the hrp-encoded secretion system.Q54589765
Analysis of the role of the Pseudomonas syringae pv. syringae HrpZ harpin in elicitation of the hypersensitive response in tobacco using functionally non-polar hrpZ deletion mutations, truncated HrpZ fragments, and hrmA mutations.Q54594313
Complementation of deletion mutations in a cloned functional cluster of Erwinia chrysanthemi out genes with Erwinia carotovora out homologues reveals OutC and OutD as candidate gatekeepers of species-specific secretion of proteins via the type II paQ71662127
AVRXa10 protein is in the cytoplasm of Xanthomonas oryzae pv. oryzaeQ72585981
Bacterial Pathogens in Plants: Life up against the WallQ74806141
Bacterial avirulence genesQ79760388
HrpI of Erwinia amylovora functions in secretion of harpin and is a member of a new protein familyQ24681626
Studies on transformation of Escherichia coli with plasmidsQ27860598
A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxRQ29615324
The cloned avirulence gene avrPto induces disease resistance in tomato cultivars containing the Pto resistance geneQ33206962
Erwinia amylovora secretes DspE, a pathogenicity factor and functional AvrE homolog, through the Hrp (type III secretion) pathwayQ33729324
A new family of RSF1010-derived expression and lac-fusion broad-host-range vectors for gram-negative bacteriaQ33770836
Erwinia amylovora secretes harpin via a type III pathway and contains a homolog of yopN of Yersinia spp.Q35604121
Altered localization of HrpZ in Pseudomonas syringae pv. syringae hrp mutants suggests that different components of the type III secretion pathway control protein translocation across the inner and outer membranes of gram-negative bacteriaQ35624414
The type III (Hrp) secretion pathway of plant pathogenic bacteria: trafficking harpins, Avr proteins, and deathQ35630003
Homology and functional similarity of an hrp-linked pathogenicity locus, dspEF, of Erwinia amylovora and the avirulence locus avrE of Pseudomonas syringae pathovar tomatoQ35807063
Expression of the avirulence gene avrBs3 from Xanthomonas campestris pv. vesicatoria is not under the control of hrp genes and is independent of plant factorsQ36165893
Molecular cloning of a Pseudomonas syringae pv. syringae gene cluster that enables Pseudomonas fluorescens to elicit the hypersensitive response in tobacco plantsQ36219048
Characterization and expression of two avirulence genes cloned from Pseudomonas syringae pv. glycineaQ36219531
Erwinia chrysanthemi hrp genes and their involvement in soft rot pathogenesis and elicitation of the hypersensitive response.Q36725558
ESTIMATION OF THE LOW-DENSITY (BETA) LIPOPROTEINS OF SERUM IN HEALTH AND DISEASE USING LARGE MOLECULAR WEIGHT DEXTRAN SULPHATEQ36730894
Molecular recognition of pathogen attack occurs inside of plant cells in plant disease resistance specified by the Arabidopsis genes RPS2 and RPM1.Q37098663
Cloned Erwinia chrysanthemi out genes enable Escherichia coli to selectively secrete a diverse family of heterologous proteins to its milieuQ37394118
Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteriaQ37608718
Gene-for-gene complementarity in plant-pathogen interactionsQ37794706
External loops at the C terminus of Erwinia chrysanthemi pectate lyase C are required for species-specific secretion through the out type II pathway.Q39565016
Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and MuQ39605636
The hrpA and hrpC operons of Erwinia amylovora encode components of a type III pathway that secretes harpinQ39844794
Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syringae pv. glycineaQ39963641
Customized secretion chaperones in pathogenic bacteriaQ41057356
The Yersinia Yop virulon: a bacterial system for subverting eukaryotic cellsQ41390851
DspA, an essential pathogenicity factor of Erwinia amylovora showing homology with AvrE of Pseudomonas syringae, is secreted via the Hrp secretion pathway in a DspB-dependent way.Q41487148
A mRNA signal for the type III secretion of Yop proteins by Yersinia enterocoliticaQ41487461
P433issue17
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
Pseudomonas syringaeQ311202
Erwinia chrysanthemiQ62896177
P304page(s)10206-10211
P577publication date1998-08-01
P1433published inProceedings of the National Academy of Sciences of the United States of AmericaQ1146531
P1476titleA cloned Erwinia chrysanthemi Hrp (type III protein secretion) system functions in Escherichia coli to deliver Pseudomonas syringae Avr signals to plant cells and to secrete Avr proteins in culture
P478volume95

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cites work (P2860)
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Q43009463A host-specific virulence protein of Erwinia herbicola pv. gypsophilae is translocated into human epithelial cells by the Type III secretion system of enteropathogenic Escherichia coli
Q34048332Analysis of putative Chlamydia trachomatis chaperones Scc2 and Scc3 and their use in the identification of type III secretion substrates.
Q36088671Bioinformatics, genomics and evolution of non-flagellar type-III secretion systems: a Darwinian perspective
Q34334292Calcium-regulated type III secretion of Yop proteins by an Escherichia coli hha mutant carrying a Yersinia pestis pCD1 virulence plasmid
Q34193678Characterization of Salmonella type III secretion hyper-activity which results in biofilm-like cell aggregation
Q41670828Characterization of the Pseudomonas syringae pv. tomato AvrRpt2 protein: demonstration of secretion and processing during bacterial pathogenesis
Q34774479Discovery of plant phenolic compounds that act as type III secretion system inhibitors or inducers of the fire blight pathogen, Erwinia amylovora.
Q42122363Diverse AvrPtoB homologs from several Pseudomonas syringae pathovars elicit Pto-dependent resistance and have similar virulence activities
Q34645388Diverse evolutionary mechanisms shape the type III effector virulence factor repertoire in the plant pathogen Pseudomonas syringae
Q57266282Eukaryotic Fatty Acylation Drives Plasma Membrane Targeting and Enhances Function of Several Type III Effector Proteins from Pseudomonas syringae
Q40945901Expression profiling of virulence and pathogenicity genes of Xanthomonas axonopodis pv. citri.
Q34257073Functional and computational analysis of amino acid patterns predictive of type III secretion system substrates in Pseudomonas syringae
Q33536582Gene-for-gene interactions: bacterial avirulence proteins specify plant disease resistance.
Q34960211Genomic mining type III secretion system effectors in Pseudomonas syringae yields new picks for all TTSS prospectors
Q30852463HecA, a member of a class of adhesins produced by diverse pathogenic bacteria, contributes to the attachment, aggregation, epidermal cell killing, and virulence phenotypes of Erwinia chrysanthemi EC16 on Nicotiana clevelandii seedlings
Q35949246Identification of Pseudomonas syringae pv. syringae 61 type III secretion system Hrp proteins that can travel the type III pathway and contribute to the translocation of effector proteins into plant cells
Q37293186Molecular signals required for type III secretion and translocation of the Xanthomonas campestris AvrBs2 protein to pepper plants
Q37356201Multiple activities of the plant pathogen type III effector proteins WtsE and AvrE require WxxxE motifs
Q58111768Novel Effector Protein EspY3 of Type III Secretion System from Enterohemorrhagic Is Localized in Actin Pedestals
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Q40387827Pseudomonas syringae type III secretion system targeting signals and novel effectors studied with a Cya translocation reporter
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Q37766931Soft rot erwiniae: from genes to genomes
Q33992662The Avr (effector) proteins HrmA (HopPsyA) and AvrPto are secreted in culture from Pseudomonas syringae pathovars via the Hrp (type III) protein secretion system in a temperature- and pH-sensitive manner.
Q35699642The Pseudomonas syringae Hrp pathogenicity island has a tripartite mosaic structure composed of a cluster of type III secretion genes bounded by exchangeable effector and conserved effector loci that contribute to parasitic fitness and pathogenicity
Q53871902The ShcA protein is a molecular chaperone that assists in the secretion of the HopPsyA effector from the type III (Hrp) protein secretion system of Pseudomonas syringae.
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Q42445169WtsE, an AvrE-family type III effector protein of Pantoea stewartii subsp. stewartii, causes cell death in non-host plants.
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Q39678327YopD and LcrH regulate expression of Yersinia enterocolitica YopQ by a posttranscriptional mechanism and bind to yopQ RNA.

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