Determining bacteriophage endopeptidase activity using either fluorophore-quencher labeled peptides combined with liquid chromatography-mass spectrometry (LC-MS) or Förster resonance energy transfer (FRET) assays

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Determining bacteriophage endopeptidase activity using either fluorophore-quencher labeled peptides combined with liquid chromatography-mass spectrometry (LC-MS) or Förster resonance energy transfer (FRET) assays is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1371/JOURNAL.PONE.0173919
P932PMC publication ID5352010
P698PubMed publication ID28296948

P50authorRolf LoodQ87922609
P2093author name stringVincent A Fischetti
Henrik Molina
P2860cites workHighly adaptable and sensitive protease assay based on fluorescence resonance energy transfer.Q53221563
Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus.Q53588360
A new lysozyme assay based on fluorescence polarization or fluorescence intensity utilizing a fluorescent peptidoglycan substrateQ71440038
Bacteriophage lysins as effective antibacterialsQ24648457
Multiple enzymatic activities of the murein hydrolase from staphylococcal phage phi11. Identification of a D-alanyl-glycine endopeptidase activityQ28144392
Biochemical and biophysical characterization of PlyGRCS, a bacteriophage endolysin active against methicillin-resistant Staphylococcus aureusQ30009348
Skyline: an open source document editor for creating and analyzing targeted proteomics experimentsQ30080030
Cell wall-targeting domain of glycylglycine endopeptidase distinguishes among peptidoglycan cross-bridgesQ30160087
Synergism between a novel chimeric lysin and oxacillin protects against infection by methicillin-resistant Staphylococcus aureusQ33525230
Surface proteins of gram-positive bacteria and mechanisms of their targeting to the cell wall envelopeQ33538951
A highly active and negatively charged Streptococcus pyogenes lysin with a rare D-alanyl-L-alanine endopeptidase activity protects mice against streptococcal bacteremiaQ33797903
A peptide-based fluorescence resonance energy transfer assay for Bacillus anthracis lethal factor proteaseQ34067110
The antimicrobial resistance crisis: causes, consequences, and managementQ34192579
FRET-based and other fluorescent proteinase probes.Q35086168
Novel phage lysin capable of killing the multidrug-resistant gram-negative bacterium Acinetobacter baumannii in a mouse bacteremia modelQ35168859
Bacteriophage lytic enzymes: novel anti-infectivesQ36242302
Structural characterization of peptidoglycan muropeptides by matrix-assisted laser desorption ionization mass spectrometry and postsource decay analysisQ36864633
Novel bacteriophage lysin with broad lytic activity protects against mixed infection by Streptococcus pyogenes and methicillin-resistant Staphylococcus aureusQ37026612
Use of 4-sulfophenyl isothiocyanate labeling and mass spectrometry to determine the site of action of the streptococcolytic peptidoglycan hydrolase zoocin AQ37032910
Lysostaphin: an antistaphylococcal agentQ37209589
Visualization of human immunodeficiency virus protease inhibition using a novel Förster resonance energy transfer molecular probeQ38267082
Analysis of peptidoglycan structure from vegetative cells of Bacillus subtilis 168 and role of PBP 5 in peptidoglycan maturation.Q39496321
Rapid killing of Streptococcus pneumoniae with a bacteriophage cell wall hydrolaseQ43820230
Development of a novel fluorescent substrate for Autolysin E, a bacterial type II amidase.Q46089091
Lack of development of new antimicrobial drugs: a potential serious threat to public healthQ48002635
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P4510describes a project that usesliquid chromatography–mass spectrometryQ873864
P433issue3
P407language of work or nameEnglishQ1860
P921main subjectbacteriophageQ165028
P304page(s)e0173919
P577publication date2017-03-15
P1433published inPLOS OneQ564954
P1476titleDetermining bacteriophage endopeptidase activity using either fluorophore-quencher labeled peptides combined with liquid chromatography-mass spectrometry (LC-MS) or Förster resonance energy transfer (FRET) assays
P478volume12

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cites work (P2860)
Q92264517Exebacase in Addition to Daptomycin Is More Active than Daptomycin or Exebacase Alone in Methicillin-Resistant Staphylococcus aureus Osteomyelitis in Rats
Q92050162Implications of Bacteriophage- and Bacteriophage Component-Based Therapies for the Clinical Microbiology Laboratory
Q92764958Postantibiotic and Sub-MIC Effects of Exebacase (Lysin CF-301) Enhance Antimicrobial Activity against Staphylococcus aureus

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