Comprehensive study of liposome-assisted synthesis of membrane proteins using a reconstituted cell-free translation system.

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Comprehensive study of liposome-assisted synthesis of membrane proteins using a reconstituted cell-free translation system. is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1038/SREP18025
P932PMC publication ID4678891
P698PubMed publication ID26667602
P5875ResearchGate publication ID287203883

P50authorMitsuru AndoQ60392129
Kazunari AkiyoshiQ105772006
Takuya UedaQ40394137
Hideki TaguchiQ43293157
P2093author name stringShugo Nakamura
Yoshihiro Sasaki
Tatsuya Niwa
Shinichi Sawada
Eri Uemura
Minato Akiyama
Sada-atu Mukai
P2860cites workWheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complexQ24309572
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Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression systemQ30160050
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Recent advances in producing and selecting functional proteins by using cell-free translationQ31972407
High-level expression of soluble human beta-defensin-2 fused with green fluorescent protein in Escherichia coli cell-free systemQ50753120
Functional analysis of membranous Fo-a subunit of F1Fo-ATP synthase by in vitro protein synthesis.Q54346375
Amphiphilic polysaccharide nanogels as artificial chaperones in cell-free protein synthesis.Q54366783
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Chaperone-assisted folding of a single-chain antibody in a reconstituted translation system.Q54501560
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Functional cell-free synthesis of a seven helix membrane protein: in situ insertion of bacteriorhodopsin into liposomesQ80515596
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Synthesis and in situ insertion of a site-specific fluorescently labeled membrane protein into cell-sized liposomesQ84569511
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In vitro synthesis of the E. coli Sec translocon from DNAQ88012778
Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system.Q33868233
Biology under construction: in vitro reconstitution of cellular function.Q34118587
Co-translational association of cell-free expressed membrane proteins with supplied lipid bilayersQ34282941
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Mechanisms of integral membrane protein insertion and foldingQ35118815
Global analysis of chaperone effects using a reconstituted cell-free translation system.Q36061318
Bimodal protein solubility distribution revealed by an aggregation analysis of the entire ensemble of Escherichia coli proteinsQ37132799
Membrane protein expression: no cells requiredQ37555379
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Production of membrane proteins without cells or detergents.Q37775137
Surface plasmon resonance spectroscopy for characterisation of membrane protein-ligand interactions and its potential for drug discoveryQ38105839
Energetics of membrane protein foldingQ38217499
Membrane protein synthesis in cell-free systems: from bio-mimetic systems to bio-membranesQ38220437
Folding and stability of integral membrane proteins in amphipolsQ38275569
Membrane assembly of the functional KcsA potassium channel in a vesicle-based eukaryotic cell-free translation system.Q39004706
Direct formation of proteo-liposomes by in vitro synthesis and cellular cytosolic delivery with connexin-expressing liposomes.Q39855332
Liposomes-mediated delivery of pro-apoptotic therapeutic membrane proteinsQ40018821
AAindex: amino acid index database, progress report 2008Q42002245
Production of functional bacteriorhodopsin by an Escherichia coli cell-free protein synthesis system supplemented with steroid detergent and lipidQ42691298
Cell-free translation reconstituted with purified componentsQ43692142
Lipid-protein nanodiscs promote in vitro folding of transmembrane domains of multi-helical and multimeric membrane proteinsQ45410607
Direct preparation of giant proteo-liposomes by in vitro membrane protein synthesisQ46977758
Properties and identification of human protein drug targetsQ48301947
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P407language of work or nameEnglishQ1860
P921main subjectmembrane proteinQ423042
P304page(s)18025
P577publication date2015-12-15
P1433published inScientific ReportsQ2261792
P1476titleComprehensive study of liposome-assisted synthesis of membrane proteins using a reconstituted cell-free translation system
P478volume5

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cites work (P2860)
Q91714144Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology
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Q50312615Functional G-Protein-Coupled Receptor (GPCR) Synthesis: The Pharmacological Analysis of Human Histamine H1 Receptor (HRH1) Synthesized by a Wheat Germ Cell-Free Protein Synthesis System Combined with Asolectin Glycerosomes
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Q57823583Proteoliposome Engineering with Cell-Free Membrane Protein Synthesis: Control of Membrane Protein Sorting into Liposomes by Chaperoning Systems
Q93163758The hallmarks of living systems: towards creating artificial cells

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