Selection of highly efficient sgRNAs for CRISPR/Cas9-based plant genome editing

scientific article published on 19 February 2016

Selection of highly efficient sgRNAs for CRISPR/Cas9-based plant genome editing is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1038/SREP21451
P932PMC publication ID4759811
P698PubMed publication ID26891616
P5875ResearchGate publication ID295246349

P2093author name stringDiqiu Yu
Gang Liang
Huimin Zhang
Dengji Lou
P2860cites workGenome-wide prediction of highly specific guide RNA spacers for CRISPR-Cas9-mediated genome editing in model plants and major crops.Q52422933
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Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana.Q52423023
Efficient gene editing in tomato in the first generation using the clustered regularly interspaced short palindromic repeats/CRISPR-associated9 system.Q52423157
Rapid Decoding of Sequence-Specific Nuclease-Induced Heterozygous and Biallelic Mutations by Direct Sequencing of PCR ProductsQ86882233
Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAsQ21559464
Mfold web server for nucleic acid folding and hybridization predictionQ24498105
Multiplex genome engineering using CRISPR/Cas systemsQ24609428
CRISPR RNA maturation by trans-encoded small RNA and host factor RNase IIIQ24628207
A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial ImmunityQ24669850
Crystal Structure of Cas9 in Complex with Guide RNA and Target DNAQ27681727
DNA targeting specificity of RNA-guided Cas9 nucleasesQ29615793
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cellsQ29616045
Genetic screens in human cells using the CRISPR-Cas9 systemQ29617411
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9.Q33931841
Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cellsQ34093413
Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in riceQ34248901
Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA.Q34323082
Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation.Q34436638
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleasesQ34454104
A CRISPR/Cas9 toolkit for multiplex genome editing in plantsQ34676725
Short motif sequences determine the targets of the prokaryotic CRISPR defence systemQ34953078
Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.Q35212505
Sequence determinants of improved CRISPR sgRNA designQ35876047
Efficient inversions and duplications of mammalian regulatory DNA elements and gene clusters by CRISPR/Cas9.Q35914859
Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in ArabidopsisQ37674388
Cas9-Guide RNA Directed Genome Editing in Soybean.Q38423192
U6 snRNA genes of Arabidopsis are transcribed by RNA polymerase III but contain the same two upstream promoter elements as RNA polymerase II-transcribed U-snRNA genesQ40517529
A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot PlantsQ41008314
Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9.Q42055857
Application of the CRISPR-Cas system for efficient genome engineering in plantsQ42573785
RNA-guided genome editing in plants using a CRISPR-Cas system.Q43649257
A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional RegulationQ44859216
Efficient Virus-Mediated Genome Editing in Plants Using the CRISPR/Cas9 SystemQ45186595
Targeted genome modification of crop plants using a CRISPR-Cas system.Q47822803
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P407language of work or nameEnglishQ1860
P921main subjectCRISPRQ412563
Cas9Q16965677
CRISPR-Cas methodQ17310682
P304page(s)21451
P577publication date2016-02-19
P1433published inScientific ReportsQ2261792
P1476titleSelection of highly efficient sgRNAs for CRISPR/Cas9-based plant genome editing
P478volume6

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cites work (P2860)
Q41358737A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants.
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