Purification of proteins fused to glutathione S-transferase

scientific article published on January 1, 2011

Purification of proteins fused to glutathione S-transferase is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1007/978-1-60761-913-0_14
P953full work available at URLhttps://europepmc.org/articles/pmc3584333?pdf=render
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20978970/?tool=EBI
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20978970/pdf/?tool=EBI
https://europepmc.org/articles/PMC3584333
https://doi.org/10.1007/978-1-60761-913-0_14
https://europepmc.org/articles/PMC3584333?pdf=render
P932PMC publication ID3584333
P698PubMed publication ID20978970

P2093author name stringSandra Harper
David W. Speicher
P2860cites workSingle-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferaseQ27860571
Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferaseQ28131695
Solubilization and refolding of bacterial inclusion body proteinsQ28277733
Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteinsQ29615167
Vectors for the inducible overexpression of glutathione S-transferase fusion proteins in yeastQ29618547
New vectors for high level expression of recombinant proteins in bacteria.Q30341857
Structural analysis of regulatory protein domains using GST-fusion proteinsQ34466476
Crystal structures of fusion proteins with large-affinity tagsQ35161672
Glutathione-S-transferase-fusion based assays for studying protein-protein interactionsQ35738259
Hydrogel-based protein nanoarraysQ38299377
Recombinant baculovirus vectors expressing glutathione-S-transferase fusion proteinsQ41539893
Comparison of phage pIII, pVIII and GST as carrier proteins for peptide immunisation in Balb/c miceQ43766116
High-throughput analysis of GST-fusion protein expression and activity-dependent protein interactions on GST-fusion protein arrays with a spectral surface plasmon resonance biosensorQ46886720
Localization and characterization of protein-protein interaction sitesQ46968964
Isolation, solubilization, refolding, and chromatographic purification of human growth hormone from inclusion bodies of Escherichia coli cells: a case study.Q54481207
An improved procedure for the purification of protein fused with glutathione S-transferaseQ67979721
Reduced background expression and improved plasmid stability with pET vectors in BL21 (DE3)Q73318089
Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instabilityQ74386329
P407language of work or nameEnglishQ1860
P921main subjectglutathioneQ116907
P304page(s)259-280
P577publication date2011-01-01
P1433published inMethods in Molecular BiologyQ15752859
P1476titlePurification of proteins fused to glutathione S-transferase
P478volume681

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