Conformational Mobility in Cytochrome P450 3A4 Explored by Pressure-Perturbation EPR Spectroscopy

scientific article published on April 2016

Conformational Mobility in Cytochrome P450 3A4 Explored by Pressure-Perturbation EPR Spectroscopy is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1016/J.BPJ.2016.02.026
P932PMC publication ID4833771
P698PubMed publication ID27074675

P50authorWayne L. HubbellQ7976400
P2093author name stringJames R Halpert
Zhongyu Yang
Dmitri R Davydov
Nadezhda Davydova
P2860cites workCrystal structure of human cytochrome P450 2D6Q24298673
Crystal structures of human cytochrome P450 3A4 bound to metyrapone and progesteroneQ24299505
Structural and mechanistic insights into the interaction of cytochrome P4503A4 with bromoergocryptine, a type I ligandQ24598661
The challenges of dealing with promiscuous drug-metabolizing enzymes, receptors and transportersQ25112732
Mammalian microsomal cytochrome P450 monooxygenase: structural adaptations for membrane binding and functional diversityQ27621446
Pivotal role of water in the mechanism of P450BM-3Q27635979
Substrate recognition and molecular mechanism of fatty acid hydroxylation by cytochrome P450 from Bacillus subtilis. Crystallographic, spectroscopic, and mutational studiesQ27640290
The 1.92-A structure of Streptomyces coelicolor A3(2) CYP154C1. A new monooxygenase that functionalizes macrolide ring systemsQ27640294
Crystal structure of human cytochrome P450 2C9 with bound warfarinQ27641685
An open conformation of mammalian cytochrome P450 2B4 at 1.6-Å resolutionQ27642370
Structure of human microsomal cytochrome P450 2C8. Evidence for a peripheral fatty acid binding siteQ27642793
Crystal Structures of Cytochrome P450 2B4 in Complex with the Inhibitor 1-Biphenyl-4-methyl-1 H -imidazole: Ligand-Induced Structural Response through α-Helical Repositioning ,Q27655307
P450cin Active Site Water: Implications for Substrate Binding and Solvent AccessibilityQ27678939
Anion-Dependent Stimulation of CYP3A4 MonooxygenaseQ27701001
Pressure effects on intra- and intermolecular interactions within proteins.Q30330245
The ins and outs of cytochrome P450s.Q30356342
Structural features of cytochromes P450 and ligands that affect drug metabolism as revealed by X-ray crystallography and NMR.Q30396630
High pressure effects on protein structure and functionQ30423793
Peripheral ligand-binding site in cytochrome P450 3A4 located with fluorescence resonance energy transfer (FRET)Q30511797
Adaptations to High Hydrostatic PressureQ33427720
How does the reductase help to regulate the catalytic cycle of cytochrome P450 3A4 using the conserved water channel?Q33817658
Identifying conformational changes with site-directed spin labelingQ34019511
Effects of high hydrostatic pressures on living cells: a consequence of the properties of macromolecules and macromolecule-associated waterQ34073329
Role of active site water molecules and substrate hydroxyl groups in oxygen activation by cytochrome P450 158A2: a new mechanism of proton transferQ34461465
High-pressure EPR reveals conformational equilibria and volumetric properties of spin-labeled proteinsQ34534205
The effects of osmotic and hydrostatic pressures on macromolecular systemsQ34620000
Revisiting volume changes in pressure-induced protein unfolding.Q34620061
High pressure, a tool for exploring heme protein active sitesQ34620097
Interactions among cytochromes P450 in microsomal membranes: oligomerization of cytochromes P450 3A4, 3A5, and 2E1 and its functional consequencesQ35055663
A large-scale allosteric transition in cytochrome P450 3A4 revealed by luminescence resonance energy transfer (LRET).Q35078270
Activation volumes in enzymic catalysis: their sources and modification by low-molecular-weight solutesQ35085264
Model-independent decomposition of two-state dataQ35086625
Protein hydration changes during catalysis: a new mechanism of enzymic rate-enhancement and ion activation/inhibition of catalysisQ35087304
A survey of active site access channels in cytochromes P450.Q35818833
Estimating hydration changes upon biomolecular reactions from osmotic stress, high pressure, and preferential hydration experiments.Q36017150
Identification of a functional water channel in cytochrome P450 enzymes.Q36032795
Energetics of heterotropic cooperativity between alpha-naphthoflavone and testosterone binding to CYP3A4Q36113874
Analysis of human cytochrome P450 3A4 cooperativity: construction and characterization of a site-directed mutant that displays hyperbolic steroid hydroxylation kineticsQ36481303
Substrate binding to cytochromes P450.Q37214645
Circular dichroism and site-directed spin labeling reveal structural and dynamical features of high-pressure states of myoglobinQ37377420
Plasticity of CYP2B enzymes: structural and solution biophysical methodsQ37429225
Pivotal role of P450-P450 interactions in CYP3A4 allostery: the case of α-naphthoflavoneQ37483394
Environmental effects in computational spectroscopy: accuracy and interpretation.Q37722646
Conformational plasticity and structure/function relationships in cytochromes P450.Q37743467
Is there a relationship between the substrate preferences and structural flexibility of cytochromes P450?Q37972328
Conformational diversity and ligand tunnels of mammalian cytochrome P450sQ38099062
The effect of high pressure upon proteins and other biomoleculesQ40154461
High pressure effects on proteins and other biomoleculesQ40335414
Deconvolutions based on singular value decomposition and the pseudoinverse: a guide for beginnersQ40736570
P450s: structural similarities and functional differences.Q40993726
CYP261 enzymes from deep sea bacteria: a clue to conformational heterogeneity in cytochromes P450.Q41888032
Role of subunit interactions in P450 oligomers in the loss of homotropic cooperativity in the cytochrome P450 3A4 mutant L211F/D214E/F304W.Q42094327
Cytochrome P450 from Photobacterium profundum SS9, a piezophilic bacterium, exhibits a tightened control of water access to the active siteQ42173613
Allosteric mechanisms in cytochrome P450 3A4 studied by high-pressure spectroscopy: pivotal role of substrate-induced changes in the accessibility and degree of hydration of the heme pocket.Q42173769
Mechanism of interactions of alpha-naphthoflavone with cytochrome P450 3A4 explored with an engineered enzyme bearing a fluorescent probe.Q42173777
Kinetics of dithionite-dependent reduction of cytochrome P450 3A4: heterogeneity of the enzyme caused by its oligomerization.Q42173805
Stochastic ensembles, conformationally adaptive teamwork, and enzymatic detoxificationQ42731458
Comparative investigation of superoxide trapping by cyclic nitrone spin traps: the use of singular value decomposition and multiple linear regression analysisQ44627934
Conformational heterogeneity of cytochrome P450 3A4 revealed by high pressure spectroscopy.Q44663408
Kinetics and thermodynamics of ligand binding by cytochrome P450 3A4.Q46935182
How large are the volume changes accompanying protein transitions and binding?Q47740169
Hydrophobic effects on partial molar volume.Q51975982
High-pressure-induced transitions in microsomal cytochrome P450 2B4 in solution: evidence for conformational inhomogeneity in the oligomers.Q52335945
Component resolution using multilinear models.Q52359467
Dynamics of Water Molecules in the Active-Site Cavity of Human Cytochromes P450Q56975735
Water as biocatalyst in cytochrome P450Q57397808
Protein Dynamics Tightly Connected to the Dynamics of Surrounding and Internal Water MoleculesQ57814723
Hydrophobic interaction electrophoresis under high hydrostatic pressure: study of the effects of pressure upon the interaction of serum albumin with a long-chain aliphatic ligandQ68047031
High affinity of ergopeptides for cytochromes P450 3A. Importance of their peptide moiety for P450 recognition and hydroxylation of bromocriptineQ72119079
The Pressure Dependence of the Spin Equilibrium in Camphor-Bound Ferric Cytochrome P-450Q72935909
Partial volumes and compressibilities of extended polypeptide chains in aqueous solution: additivity scheme and implication of protein unfolding at normal and high pressureQ73581673
Allosteric behavior in cytochrome p450-dependent in vitro drug-drug interactions: a prospective based on conformational dynamicsQ73755989
Dynamics of protein-bound water in the heme domain of P450BM3 studied by high-pressure spectroscopy: comparison with P450cam and P450 2B4Q77795407
High pressure: a new tool to study P450 structure and functionQ78496564
Multiple sequential steps involved in the binding of inhibitors to cytochrome P450 3A4Q79475811
Allosteric P450 mechanisms: multiple binding sites, multiple conformers or both?Q82758182
A singular value decomposition approach for kinetic analysis of reactions of HNO with myoglobinQ85404566
P433issue7
P407language of work or nameEnglishQ1860
P1104number of pages14
P304page(s)1485-1498
P577publication date2016-04-01
P1433published inBiophysical JournalQ2032955
P1476titleConformational Mobility in Cytochrome P450 3A4 Explored by Pressure-Perturbation EPR Spectroscopy
P478volume110

Reverse relations

cites work (P2860)
Q58796074Effects of polymorphic variation on the thermostability of heterogenous populations of CYP3A4 and CYP2C9 enzymes in solution
Q46445841High-Level Production and Properties of the Cysteine-Depleted Cytochrome P450 3A4.
Q50050835High-pressure EPR spectroscopy studies of the E. coli lipopolysaccharide transport proteins LptA and LptC.
Q37611698Physical Studies of P450-P450 Interactions: Predicting Quaternary Structures of P450 Complexes in Membranes from Their X-ray Crystal Structures
Q89353053Steroid bioconjugation to a CYP3A4 allosteric site and its effect on substrate binding and coupling efficiency
Q92860132Structural and dynamic origins of ESR lineshapes in spin-labeled GB1 domain: the insights from spin dynamics simulations based on long MD trajectories

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