Poliovirus proteinase 3C: large-scale expression, purification, and specific cleavage activity on natural and synthetic substrates in vitro

scientific article published on December 1988

Poliovirus proteinase 3C: large-scale expression, purification, and specific cleavage activity on natural and synthetic substrates in vitro is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1128/JVI.62.12.4586-4593.1988
P932PMC publication ID254243
P698PubMed publication ID2846872

P2093author name stringE Wimmer
P V Pallai
M J Nicklin
K S Harris
P2860cites workL-trans-Epoxysuccinyl-leucylamido(4-guanidino)butane (E-64) and its analogues as inhibitors of cysteine proteinases including cathepsins B, H and LQ24531160
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genesQ27860623
Poliovirus-encoded proteinase 3C: a possible evolutionary link between cellular serine and cysteine proteinase familiesQ28286937
Vectors for selective expression of cloned DNAs by T7 RNA polymeraseQ29620230
Association of poliovirus proteins with the endoplasmic reticulumQ30503119
Identification of poliovirus polypeptide P63 as a soluble RNA-dependent RNA polymeraseQ33925388
Encephalomyocarditis virus 3C protease: efficient cell-free expression from clones which link viral 5' noncoding sequences to the P3 regionQ33926932
Processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structureQ34162845
Primary structure, gene organization and polypeptide expression of poliovirus RNA.Q34252540
Capsid protein VP4 of poliovirus is N-myristoylatedQ34362739
Purification and partial characterization of poliovirus protease 2A by means of a functional assayQ34553558
Amino-terminal processing of mutant forms of yeast iso-1-cytochrome c. The specificities of methionine aminopeptidase and acetyltransferaseQ34564166
Implications of the picornavirus capsid structure for polyprotein processingQ34583851
Poliovirus polypeptide precursors: expression in vitro and processing by exogenous 3C and 2A proteinasesQ34630609
Similarity in gene organization and homology between proteins of animal picornaviruses and a plant comovirus suggest common ancestry of these virus familiesQ35444105
Protein processing map of poliovirusQ36913264
Poliovirus proteinase 2A induces cleavage of eucaryotic initiation factor 4F polypeptide p220.Q36918487
Expression and site-specific mutagenesis of the poliovirus 3C protease in Escherichia coliQ37391477
Strategy and tactics in protein chemistryQ39995528
Systematic nomenclature of picornavirus proteinsQ40140317
Translation of Encephalomyocarditis Virus RNA in vitro Yields an Active Proteolytic Processing EnzymeQ40145381
In vitro molecular genetics as a tool for determining the differential cleavage specificities of the poliovirus 3C proteinaseQ40565594
Evidence for histidine in the active site of papainQ41886439
A second virus-encoded proteinase involved in proteolytic processing of poliovirus polyprotein.Q42028125
Myristylation of picornavirus capsid protein VP4 and its structural significanceQ43521225
Protein 3CD is the major poliovirus proteinase responsible for cleavage of the P1 capsid precursorQ44133857
Expression of a cloned gene segment of poliovirus in E. coli: Evidence for autocatalytic production of the viral proteinaseQ44915778
A detailed kinetic analysis of the in vitro synthesis and processing of encephalomyocarditis virus productsQ45837350
Complete nucleotide sequences of all three poliovirus serotype genomes. Implication for genetic relationship, gene function and antigenic determinantsQ48391182
Poliovirus protein 3CD is the active protease for processing of the precursor protein P1 in vitroQ69832090
Biochemical evidence for intertypic genetic recombination of poliovirusesQ72862176
P433issue12
P407language of work or nameEnglishQ1860
P921main subjectPoliovirusQ70923324
P304page(s)4586-4593
P577publication date1988-12-01
P1433published inJournal of VirologyQ1251128
P1476titlePoliovirus proteinase 3C: large-scale expression, purification, and specific cleavage activity on natural and synthetic substrates in vitro
P478volume62

Reverse relations

cites work (P2860)
Q36686958A cellular cofactor facilitates efficient 3CD cleavage of the poliovirus P1 precursor.
Q73461939A simple purification and fluorescent assay method of the poliovirus 3C protease searching for specific inhibitors
Q24627225Activity of purified biosynthetic proteinase of human immunodeficiency virus on natural substrates and synthetic peptides
Q35376497An overview of enzymatic reagents for the removal of affinity tags
Q45854802Autoproteolytic cleavage of recombinant 3C proteinase of hepatitis a virus
Q27671667Broad-spectrum antivirals against 3C or 3C-like proteases of picornaviruses, noroviruses, and coronaviruses.
Q36310354Chikungunya nsP2 protease is not a papain-like cysteine protease and the catalytic dyad cysteine is interchangeable with a proximal serine
Q36829933Chimeric picornavirus polyproteins demonstrate a common 3C proteinase substrate specificity
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Q39579789Complete protein linkage map of poliovirus P3 proteins: interaction of polymerase 3Dpol with VPg and with genetic variants of 3AB.
Q36561293DNA binding domain and subunit interactions of transcription factor IIIC revealed by dissection with poliovirus 3C protease
Q36641030Degradation of the interferon-induced 68,000-M(r) protein kinase by poliovirus requires RNA
Q34364212Determinants of the recognition of enteroviral cloverleaf RNA by coxsackievirus B3 proteinase 3C.
Q36661993Direct cleavage of human TATA-binding protein by poliovirus protease 3C in vivo and in vitro
Q36831732Effects of mutations in poliovirus 3Dpol on RNA polymerase activity and on polyprotein cleavage
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Q41153572Expression and characterization of poliovirus proteins 3BVPg, 3Cpro, and 3Dpol in recombinant baculovirus-infected Spodoptera frugiperda cells
Q43733755Expression and partial purification of recombinant tomato ringspot nepovirus 3C-like proteinase: comparison of the activity of the mature proteinase and the VPg-proteinase precursor.
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Q40107837Foot-and-mouth disease virus protease 3C induces specific proteolytic cleavage of host cell histone H3.
Q35881158Identification of active-site residues in protease 3C of hepatitis A virus by site-directed mutagenesis.
Q40045340Identification of the cleavage site and determinants required for poliovirus 3CPro-catalyzed cleavage of human TATA-binding transcription factor TBP.
Q36697516Infection of HeLa cells with poliovirus results in modification of a complex that binds to the rRNA promoter
Q35860088Inhibition of basal transcription by poliovirus: a virus- encoded protease (3Cpro) inhibits formation of TBP-TATA box complex in vitro
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Q53070919Multiple poliovirus-induced organelles suggested by comparison of spatiotemporal dynamics of membranous structures and phosphoinositides.
Q39876818Mutagenesis of the coxsackie B3 virus 2B/2C cleavage site: determinants of processing efficiency and effects on viral replication
Q40067609Mutational analysis of the proposed FG loop of poliovirus proteinase 3C identifies amino acids that are necessary for 3CD cleavage and might be determinants of a function distinct from proteolytic activity
Q36806331Myristoylation of the poliovirus polyprotein is required for proteolytic processing of the capsid and for viral infectivity
Q36655208Poliovirus capsid proteins derived from P1 precursors with glutamine-valine cleavage sites have defects in assembly and RNA encapsidation.
Q35931642Poliovirus proteinase 3C converts an active form of transcription factor IIIC to an inactive form: a mechanism for inhibition of host cell polymerase III transcription by poliovirus
Q34566220Poliovirus thiol proteinase 3C can utilize a serine nucleophile within the putative catalytic triad
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Q40398033Shutoff of RNA polymerase II transcription by poliovirus involves 3C protease-mediated cleavage of the TATA-binding protein at an alternative site: incomplete shutoff of transcription interferes with efficient viral replication
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Q33744126The structures of picornaviral proteinases.
Q39682617Translation of polioviral mRNA is inhibited by cleavage of polypyrimidine tract-binding proteins executed by polioviral 3C(pro)
Q33927954beta-Galactosidase containing a human immunodeficiency virus protease cleavage site is cleaved and inactivated by human immunodeficiency virus protease
Q36689121trans rescue of a mutant poliovirus RNA polymerase function

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