scholarly article | Q13442814 |
P50 | author | J. Keith Joung | Q28033740 |
Martin J. Aryee | Q57526614 | ||
Benjamin P Kleinstiver | Q57661613 | ||
Zachary McCaw | Q58257197 | ||
P2093 | author name string | Shengdar Q Tsai | |
Nhu T Nguyen | |||
Moira M Welch | |||
Jose M Lopez | |||
Michelle S Prew | |||
P2860 | cites work | RNA-guided human genome engineering via Cas9 | Q24598394 |
Multiplex genome engineering using CRISPR/Cas systems | Q24609428 | ||
CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III | Q24628207 | ||
Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system | Q24669821 | ||
A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity | Q24669850 | ||
Genome-editing Technologies for Gene and Cell Therapy | Q26769662 | ||
RNA-programmed genome editing in human cells | Q28044562 | ||
Development and applications of CRISPR-Cas9 for genome engineering | Q28241526 | ||
Genome editing. The new frontier of genome engineering with CRISPR-Cas9 | Q28252298 | ||
CRISPR-Cas systems for editing, regulating and targeting genomes | Q29615781 | ||
Improving CRISPR-Cas nuclease specificity using truncated guide RNAs | Q29616043 | ||
Open-source guideseq software for analysis of GUIDE-seq data. | Q31092099 | ||
Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases | Q33593394 | ||
Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo | Q33652566 | ||
An updated evolutionary classification of CRISPR-Cas systems | Q33660852 | ||
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing | Q33875102 | ||
The Cpf1 CRISPR-Cas protein expands genome-editing tools. | Q34045416 | ||
Rationally engineered Cas9 nucleases with improved specificity | Q34045530 | ||
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases | Q34454104 | ||
Engineered CRISPR-Cas9 nucleases with altered PAM specificities | Q34481737 | ||
High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. | Q34507554 | ||
Biology and Applications of CRISPR Systems: Harnessing Nature's Toolbox for Genome Engineering | Q34509196 | ||
Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases. | Q35060760 | ||
DNA-binding-domain fusions enhance the targeting range and precision of Cas9. | Q36372381 | ||
Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition | Q36398694 | ||
FLASH assembly of TALENs for high-throughput genome editing | Q36573684 | ||
Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells | Q38911544 | ||
Characterization of Staphylococcus aureus Cas9: a smaller Cas9 for all-in-one adeno-associated virus delivery and paired nickase applications. | Q41105167 | ||
Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors. | Q42174678 | ||
First indication for a functional CRISPR/Cas system in Francisella tularensis | Q46151591 | ||
P433 | issue | 8 | |
P921 | main subject | CRISPR | Q412563 |
P304 | page(s) | 869-874 | |
P577 | publication date | 2016-06-27 | |
P1433 | published in | Nature Biotechnology | Q1893837 |
P1476 | title | Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells | |
P478 | volume | 34 |
Q47601711 | A 'new lease of life': FnCpf1 possesses DNA cleavage activity for genome editing in human cells. |
Q38955410 | A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants. |
Q90657564 | A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing |
Q95324869 | A Cas9 with PAM recognition for adenine dinucleotides |
Q92861918 | A Novel Hybrid CNN-SVR for CRISPR/Cas9 Guide RNA Activity Prediction |
Q89129497 | A Single Multiplex crRNA Array for FnCpf1-Mediated Human Genome Editing |
Q52430518 | A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs. |
Q55601613 | A large-scale whole-genome sequencing analysis reveals highly specific genome editing by both Cas9 and Cpf1 (Cas12a) nucleases in rice. |
Q92578973 | A split CRISPR-Cpf1 platform for inducible genome editing and gene activation |
Q89419306 | Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize |
Q47199964 | Advances in Engineering the Fly Genome with the CRISPR-Cas System. |
Q92483205 | Allele specific repair of splicing mutations in cystic fibrosis through AsCas12a genome editing |
Q92767814 | An anti-CRISPR protein disables type V Cas12a by acetylation |
Q90591046 | Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies |
Q61804726 | Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis |
Q52431317 | Applications of CRISPR/Cas System to Bacterial Metabolic Engineering. |
Q40823601 | Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs. |
Q33705629 | BLISS is a versatile and quantitative method for genome-wide profiling of DNA double-strand breaks |
Q52430888 | Base editing with a Cpf1-cytidine deaminase fusion. |
Q46319869 | Basics of genome editing technology and its application in livestock species |
Q51206445 | Beyond CRISPR: A guide to the many other ways to edit a genome. |
Q66679292 | CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity |
Q64059789 | CRISPR Cpf1 proteins: structure, function and implications for genome editing |
Q91996453 | CRISPR Genome Editing Systems in the Genus Clostridium: a Timely Advancement |
Q39014648 | CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes |
Q90244326 | CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects |
Q49959398 | CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity |
Q97589304 | CRISPR-Cas12a with an oAd Induces Precise and Cancer-Specific Genomic Reprogramming of EGFR and Efficient Tumor Regression |
Q41357802 | CRISPR-Cas12a-Assisted Recombineering in Bacteria |
Q64085639 | CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool |
Q89540005 | CRISPR-Cpf1 Activation of Endogenous BMP4 Gene for Osteogenic Differentiation of Umbilical-Cord-Derived Mesenchymal Stem Cells |
Q46244913 | CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing |
Q64389322 | CRISPR-Cpf1-Assisted Multiplex Genome Editing and Transcriptional Repression in Streptomyces |
Q92600942 | CRISPR-LbCas12a-mediated modification of citrus |
Q54941286 | CRISPR-LbCpf1 prevents choroidal neovascularization in a mouse model of age-related macular degeneration. |
Q47103147 | CRISPR-offinder: a CRISPR guide RNA design and off-target searching tool for user-defined protospacer adjacent motif |
Q98226241 | CRISPR/Cas12a Mediated Genome Editing Enhances Bombyx mori Resistance to BmNPV |
Q47161696 | CRISPR/Cas9 Genome-Editing System in Human Stem Cells: Current Status and Future Prospects. |
Q50132399 | CRISPR/Cas9: A tool for immunological research |
Q47990831 | Cas9, Cpf1 and C2c1/2/3-What's next? |
Q52431512 | Chemically Modified Cpf1-CRISPR RNAs Mediate Efficient Genome Editing in Mammalian Cells. |
Q47441552 | Class 2 CRISPR-Cas RNA-guided endonucleases: Swiss Army knives of genome editing |
Q59127565 | Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing |
Q91041537 | Computational Analysis Concerning the Impact of DNA Accessibility on CRISPR-Cas9 Cleavage Efficiency |
Q92341595 | Computational approaches for effective CRISPR guide RNA design and evaluation |
Q92777200 | Conformational Dynamics and Cleavage Sites of Cas12a Are Modulated by Complementarity between crRNA and DNA |
Q39613576 | Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria |
Q64074687 | Cpf1 enables fast and efficient genome editing in Aspergilli |
Q30374204 | Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells. |
Q38430453 | Cpf1 proteins excise CRISPR RNAs from mRNA transcripts in mammalian cells |
Q47397445 | Cpf1-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cpf1. |
Q50066023 | Deep learning improves prediction of CRISPR-Cpf1 guide RNA activity |
Q57810989 | Defining CRISPR-Cas9 genome-wide nuclease activities with CIRCLE-seq |
Q61136274 | Direct observation of DNA target searching and cleavage by CRISPR-Cas12a |
Q59345200 | Discovery of widespread type I and type V CRISPR-Cas inhibitors |
Q63740288 | Disruptive Technology: CRISPR/Cas-Based Tools and Approaches |
Q47365243 | Diverse Class 2 CRISPR-Cas Effector Proteins for Genome Engineering Applications. |
Q34536517 | Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems |
Q36256836 | Diversity and evolution of class 2 CRISPR-Cas systems |
Q92224257 | Editor's cut: DNA cleavage by CRISPR RNA-guided nucleases Cas9 and Cas12a |
Q37460202 | Efficient targeted mutagenesis of rice and tobacco genomes using Cpf1 from Francisella novicida |
Q62490800 | Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing |
Q47094058 | Engineered Cpf1 variants with altered PAM specificities |
Q88847067 | Engineering CRISPR-Cpf1 crRNAs and mRNAs to maximize genome editing efficiency |
Q52431448 | Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems. |
Q57128439 | Engineering the Direct Repeat Sequence of crRNA for Optimization of FnCpf1-Mediated Genome Editing in Human Cells |
Q64039779 | Enhanced Cas12a editing in mammalian cells and zebrafish |
Q61799299 | Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds |
Q58771152 | Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo |
Q90389401 | Francisella novicida Cas9 interrogates genomic DNA with very high specificity and can be used for mammalian genome editing |
Q36306820 | Functional interrogation of non-coding DNA through CRISPR genome editing |
Q47119622 | Fusion guide RNAs for orthogonal gene manipulation with Cas9 and Cpf1. |
Q36372386 | GUIDEseq: a bioconductor package to analyze GUIDE-Seq datasets for CRISPR-Cas nucleases. |
Q46398292 | Gene Editing and Human Pluripotent Stem Cells: Tools for Advancing Diabetes Disease Modeling and Beta-Cell Development |
Q97567741 | Gene Therapy Intervention in Neovascular Eye Disease: A Recent Update |
Q49849235 | Gene drives to fight malaria: current state and future directions |
Q50452674 | Gene editing in mouse zygotes using the CRISPR/Cas9 system |
Q57170220 | Gene editing in the context of an increasingly complex genome |
Q33658492 | Generation of targeted mutant rice using a CRISPR-Cpf1 system |
Q55668720 | Genetic editing and interrogation with Cpf1 and caged truncated pre-tRNA-like crRNA in mammalian cells. |
Q57073075 | Genome Editing in Rice: Recent Advances, Challenges, and Future Implications |
Q51147648 | Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system. |
Q89144123 | Genome editing by natural and engineered CRISPR-associated nucleases |
Q36290952 | Genome editing for inborn errors of metabolism: advancing towards the clinic. |
Q97521544 | Genome editing of CCR5 by AsCpf1 renders CD4+T cells resistance to HIV-1 infection |
Q96641394 | Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors |
Q50086294 | Genome-Wide Profiling of DNA Double-Strand Breaks by the BLESS and BLISS Methods |
Q98463768 | Genome-wide specificity of dCpf1 cytidine base editors |
Q90298756 | Harnessing "A Billion Years of Experimentation": The Ongoing Exploration and Exploitation of CRISPR-Cas Immune Systems |
Q101455781 | Highly efficient and safe genome editing by CRISPR-Cas12a using CRISPR RNA with a ribosyl-2'-O-methylated uridinylate-rich 3'-overhang in mouse zygotes |
Q58759613 | Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3'-overhang |
Q37692030 | How to Train a Cell-Cutting-Edge Molecular Tools |
Q92934013 | Identification of Guide-Intrinsic Determinants of Cas9 Specificity |
Q45873745 | Immunity to CRISPR Cas9 and Cas12a therapeutics |
Q90298751 | Impact of Genetic Variation on CRISPR-Cas Targeting |
Q38651944 | Implications of human genetic variation in CRISPR-based therapeutic genome editing |
Q89927571 | Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases |
Q93332560 | Improvement of the CRISPR-Cpf1 system with ribozyme-processed crRNA |
Q90317559 | In vivo genome editing using the Cpf1 ortholog derived from Eubacterium eligens |
Q33807299 | In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni |
Q36227944 | In vivo high-throughput profiling of CRISPR-Cpf1 activity |
Q93112660 | Increasing the specificity of CRISPR systems with engineered RNA secondary structures |
Q91708149 | Induced Genetic Variation in Crop Plants by Random or Targeted Mutagenesis: Convergence and Differences |
Q46200383 | Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors. |
Q95266243 | Interfering with retrotransposition by two types of CRISPR effectors: Cas12a and Cas13a |
Q47161048 | In Vivo Knockout of the Vegfa Gene by Lentiviral Delivery of CRISPR/Cas9 in Mouse Retinal Pigment Epithelium Cells. |
Q39383306 | Mammalian synthetic biology in the age of genome editing and personalized medicine |
Q92796969 | Manipulation of spermatogonial stem cells in livestock species |
Q48266764 | Marker-free coselection for CRISPR-driven genome editing in human cells |
Q99207790 | Massively parallel kinetic profiling of natural and engineered CRISPR nucleases |
Q105031548 | Massively parallel kinetic profiling of natural and engineered CRISPR nucleases |
Q58708498 | Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants |
Q59357423 | Meganuclease targeting of PCSK9 in macaque liver leads to stable reduction in serum cholesterol |
Q38919610 | Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity |
Q39201233 | Methods for decoding Cas9 protospacer adjacent motif (PAM) sequences: A brief overview |
Q91594179 | Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis |
Q96167183 | Molecular mechanisms, off-target activities, and clinical potentials of genome editing systems |
Q33767636 | Multiplex gene regulation by CRISPR-ddCpf1 |
Q90596815 | Multiplexed orthogonal genome editing and transcriptional activation by Cas12a |
Q99711158 | Novel Therapeutic Approaches for the Treatment of Retinal Degenerative Diseases: Focus on CRISPR/Cas-Based Gene Editing |
Q52429666 | Nuclease-Mediated Gene Therapies for Inherited Metabolic Diseases of the Liver. |
Q91907979 | One-step generation of modular CAR-T cells with AAV-Cpf1 |
Q97531694 | Optimization of AsCas12a for combinatorial genetic screens in human cells |
Q89380038 | Optimized CRISPR-Cpf1 system for genome editing in zebrafish |
Q38716335 | Organoid technologies meet genome engineering |
Q92060000 | Overcoming challenges and dogmas to understand the functions of pseudogenes |
Q42375190 | Perfectly matched 20-nucleotide guide RNA sequences enable robust genome editing using high-fidelity SpCas9 nucleases |
Q98217495 | Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities |
Q91968214 | Pooled library screening with multiplexed Cpf1 library |
Q41051871 | Precise insertion and guided editing of higher plant genomes using Cpf1 CRISPR nucleases. |
Q64950555 | Prediction of activity and specificity of CRISPR-Cpf1 using convolutional deep learning neural networks. |
Q89579863 | Prediction of off-target activities for the end-to-end design of CRISPR guide RNAs |
Q97587427 | Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment |
Q49916186 | Primordial germ cell-mediated transgenesis and genome editing in birds |
Q37613647 | Programmable Genome Editing Tools and their Regulation for Efficient Genome Engineering |
Q37639765 | Progress in Genome Editing Technology and Its Application in Plants |
Q28079621 | Rapid Evolution of Manifold CRISPR Systems for Plant Genome Editing |
Q47198422 | Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System. |
Q98564790 | Rapid repair of human disease-specific single-nucleotide variants by One-SHOT genome editing |
Q88596052 | Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a) |
Q39227008 | Refining strategies to translate genome editing to the clinic |
Q100437380 | Robustly improved base editing efficiency of Cpf1 base editor using optimized cytidine deaminases |
Q101225482 | SHERLOCK and DETECTR: CRISPR-Cas Systems as Potential Rapid Diagnostic Tools for Emerging Infectious Diseases |
Q90242502 | SHERLOCK: nucleic acid detection with CRISPR nucleases |
Q92063627 | Selective targeting of the oncogenic KRAS G12S mutant allele by CRISPR/Cas9 induces efficient tumor regression |
Q103037446 | Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene Editing |
Q52431255 | Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells. |
Q89637245 | Specificity profiling of CRISPR system reveals greatly enhanced off-target gene editing |
Q51041590 | Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a. |
Q48035504 | Structural Basis for the Altered PAM Recognition by Engineered CRISPR-Cpf1. |
Q47828036 | Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1. |
Q50857661 | Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage. |
Q38784006 | Synthetic Biology-The Synthesis of Biology |
Q60949520 | Synthetic Oligonucleotides Inhibit CRISPR-Cpf1-Mediated Genome Editing |
Q91666030 | Synthetic chimeric nucleases function for efficient genome editing |
Q42289899 | Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering |
Q55069666 | Systematic evaluation of CRISPR-Cas systems reveals design principles for genome editing in human cells. |
Q41235149 | Targeted Disruption of V600E-Mutant BRAF Gene by CRISPR-Cpf1. |
Q42292457 | Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting |
Q55168127 | Targeting repair pathways with small molecules increases precise genome editing in pluripotent stem cells. |
Q40113930 | Technical considerations for the use of CRISPR/Cas9 in hematology research. |
Q51148515 | The Biology of CRISPR-Cas: Backward and Forward. |
Q33701679 | The CCTL (Cpf1-assisted Cutting and Taq DNA ligase-assisted Ligation) method for efficient editing of large DNA constructs in vitro |
Q47299533 | The Conspicuity of CRISPR-Cpf1 System as a Significant Breakthrough in Genome Editing |
Q64111182 | The Effect of DNA Topology on Observed Rates of R-Loop Formation and DNA Strand Cleavage by CRISPR Cas12a |
Q56876353 | The Francisella novicida Cas12a is sensitive to the structure downstream of the terminal repeat in CRISPR arrays |
Q55159081 | The Non-Coding RNA Journal Club: Highlights on Recent Papers-4. |
Q38608686 | The Power of Zebrafish in Personalised Medicine. |
Q47594743 | The Revolution Continues: Newly Discovered Systems Expand the CRISPR-Cas Toolkit. |
Q91973691 | The Rise of the CRISPR/Cpf1 System for Efficient Genome Editing in Plants |
Q37720304 | The Search for Resistance to Cassava Mosaic Geminiviruses: How Much We Have Accomplished, and What Lies Ahead |
Q58588347 | The applications of CRISPR/Cas system in molecular detection |
Q91904611 | The emerging and uncultivated potential of CRISPR technology in plant science |
Q47552046 | The evolution of CRISPR/Cas9 and their cousins: hope or hype? |
Q49335959 | The prospects of CRISPR-based genome engineering in the treatment of neurodegenerative disorders |
Q64955168 | Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d. |
Search more.