Conserved glutamate residues Glu-343 and Glu-519 provide mechanistic insights into cation/nucleoside cotransport by human concentrative nucleoside transporter hCNT3

scientific article published on 20 April 2009

Conserved glutamate residues Glu-343 and Glu-519 provide mechanistic insights into cation/nucleoside cotransport by human concentrative nucleoside transporter hCNT3 is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1074/JBC.M109.009613
P932PMC publication ID2719363
P698PubMed publication ID19380587

P50authorStephen A. BaldwinQ47037548
P2093author name stringCarol E Cass
James D Young
Edward Karpinski
Amy M L Ng
Kyla M Smith
Melissa D Slugoski
Sylvia Y M Yao
P2860cites workMolecular cloning and functional expression of cDNAs encoding a human Na+-nucleoside cotransporter (hCNT1)Q24317366
Conserved glutamate residues are critically involved in Na+/nucleoside cotransport by human concentrative nucleoside transporter 1 (hCNT1).Q48785548
Specific mutations in transmembrane helix 8 of human concentrative Na+/nucleoside cotransporter hCNT1 affect permeant selectivity and cation couplingQ48802845
Acidic residues involved in cation and substrate interactions in the Na+/dicarboxylate cotransporter, NaDC-1.Q48916125
Aspartate 55 in the Na+/proline permease of Escherichia coli is essential for Na+-coupled proline uptake.Q54567661
Functional roles of Glu-269 and Glu-325 within the lactose permease of Escherichia coliQ72670759
A conserved aspartate residue, Asp187, is important for Na+-dependent proline binding and transport by the Na+/proline transporter of Escherichia coliQ77349888
Human equilibrative nucleoside transporter (ENT) family of nucleoside and nucleobase transporter proteinsQ94705295
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Q25938983
Structure and mechanism of the lactose permease of Escherichia coliQ27641768
The Crystal Structure of a Sodium Galactose Transporter Reveals Mechanistic Insights into Na+/Sugar SymportQ27651031
Structure and Molecular Mechanism of a Nucleobase-Cation-Symport-1 Family TransporterQ27652531
The equilibrative nucleoside transporter family, SLC29Q28184224
The concentrative nucleoside transporter family, SLC28Q28186657
Molecular cloning, functional expression and chromosomal localization of a cDNA encoding a human Na+/nucleoside cotransporter (hCNT2) selective for purine nucleosides and uridineQ28372006
Molecular identification and characterization of novel human and mouse concentrative Na+-nucleoside cotransporter proteins (hCNT3 and mCNT3) broadly selective for purine and pyrimidine nucleosides (system cib)Q28511220
Identification of amino acid residues responsible for the pyrimidine and purine nucleoside specificities of human concentrative Na(+) nucleoside cotransporters hCNT1 and hCNT2Q28610373
Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transportersQ29616590
Structure of a glutamate transporter homologue from Pyrococcus horikoshiiQ30032687
Discontinuous membrane helices in transport proteins and their correlation with functionQ30158032
Neutralization of a conserved amino acid residue in the human Na+/glucose transporter (hSGLT1) generates a glucose-gated H+ channelQ31451799
A novel proton-dependent nucleoside transporter, CeCNT3, from Caenorhabditis elegans.Q31843898
Adenosine in the central nervous system: release mechanisms and extracellular concentrationsQ33955930
Single-channel currents produced by the serotonin transporter and analysis of a mutation affecting ion permeationQ34041264
Transport of physiological nucleosides and anti-viral and anti-neoplastic nucleoside drugs by recombinant Escherichia coli nucleoside-H(+) cotransporter (NupC) produced in Xenopus laevis oocytesQ34282746
Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH.Q34430086
Cation coupling properties of human concentrative nucleoside transporters hCNT1, hCNT2 and hCNT3.Q34622000
Nucleoside anticancer drugs: the role of nucleoside transporters in resistance to cancer chemotherapyQ35567292
A conformationally mobile cysteine residue (Cys-561) modulates Na+ and H+ activation of human CNT3Q35676740
Transport bicyclesQ36154771
Structure and mechanism of the lactose permeaseQ36159720
Nucleoside transporters: from scavengers to novel therapeutic targetsQ36527095
Concentrative nucleoside transporters (CNTs) in epithelia: from absorption to cell signalingQ36920475
Substituted cysteine accessibility method analysis of human concentrative nucleoside transporter hCNT3 reveals a novel discontinuous region of functional importance within the CNT family motif (G/A)XKX3NEFVA(Y/M/F).Q37285173
The use of Xenopus oocytes for the study of ion channels.Q38184933
Mutagenesis of acidic residues in putative membrane-spanning segments of the melibiose permease of Escherichia coli. I. Effect on Na(+)-dependent transport and binding propertiesQ38321677
A proton-mediated conformational shift identifies a mobile pore-lining cysteine residue (Cys-561) in human concentrative nucleoside transporter 3.Q38923874
Glutamate 346 of human Na+-H+ exchanger NHE1 is crucial for modulating both the affinity for Na+ and the interaction with amiloride derivativesQ40604944
Aspartate 345 of the dopamine transporter is critical for conformational changes in substrate translocation and cocaine bindingQ40609766
Nucleoside and nucleobase transport systems of mammalian cellsQ41290973
GAT1 (GABA:Na+:Cl-) cotransport function. Kinetic studies in giant Xenopus oocyte membrane patchesQ41839623
GAT1 (GABA:Na+:Cl-) cotransport function. Database reconstruction with an alternating access modelQ41938539
Localization of broadly selective equilibrative and concentrative nucleoside transporters, hENT1 and hCNT3, in human kidneyQ42510645
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.Q42604432
Cysteine-accessibility analysis of transmembrane domains 11-13 of human concentrative nucleoside transporter 3.Q43246854
Subcellular distribution and membrane topology of the mammalian concentrative Na+-nucleoside cotransporter rCNT1.Q43619454
Sites important for Na+ and substrate binding in the Na+/proline transporter of Escherichia coli, a member of the Na+/solute symporter familyQ43833690
An ancient prevertebrate Na+-nucleoside cotransporter (hfCNT) from the Pacific hagfish (Eptatretus stouti).Q44021405
Is the glutamate residue Glu-373 the proton acceptor of the excitatory amino acid carrier 1?Q44207688
Functional characterization of a H+/nucleoside co-transporter (CaCNT) from Candida albicans, a fungal member of the concentrative nucleoside transporter (CNT) family of membrane proteinsQ44470706
Electrophysiological characterization of a recombinant human Na+-coupled nucleoside transporter (hCNT1) produced in Xenopus oocytesQ44934641
The broadly selective human Na+/nucleoside cotransporter (hCNT3) exhibits novel cation-coupled nucleoside transport characteristicsQ46471209
Functional characterization of a nucleoside-derived drug transporter variant (hCNT3C602R) showing altered sodium-binding capacity.Q46907205
P4510describes a project that usesImageJQ1659584
P433issue25
P407language of work or nameEnglishQ1860
P304page(s)17266-17280
P577publication date2009-04-20
P1433published inJournal of Biological ChemistryQ867727
P1476titleConserved glutamate residues Glu-343 and Glu-519 provide mechanistic insights into cation/nucleoside cotransport by human concentrative nucleoside transporter hCNT3
P478volume284

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cites work (P2860)
Q27677998Crystal structure of a concentrative nucleoside transporter from Vibrio cholerae at 2.4 Å
Q43056101Effects of Na+ and H+ on steady-state and presteady-state currents of the human concentrative nucleoside transporter 3 (hCNT3).
Q46429658Functional analysis of the human concentrative nucleoside transporter-1 variant hCNT1S546P provides insight into the sodium-binding pocket
Q38698643Human Concentrative Nucleoside Transporter 3 (hCNT3, SLC28A3) Forms a Cyclic Homotrimer.
Q27684896Structural basis of nucleoside and nucleoside drug selectivity by concentrative nucleoside transporters
Q41999847Substituted cysteine accessibility method (SCAM) analysis of the transport domain of human concentrative nucleoside transporter 3 (hCNT3) and other family members reveals features of structural and functional importance
Q37285173Substituted cysteine accessibility method analysis of human concentrative nucleoside transporter hCNT3 reveals a novel discontinuous region of functional importance within the CNT family motif (G/A)XKX3NEFVA(Y/M/F).
Q37834510Transporters that translocate nucleosides and structural similar drugs: structural requirements for substrate recognition
Q42349026Visualizing multistep elevator-like transitions of a nucleoside transporter

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