A novel bicistronic vector for overexpressing Mycobacterium tuberculosis proteins in Escherichia coli

scientific article published on 30 December 2008

A novel bicistronic vector for overexpressing Mycobacterium tuberculosis proteins in Escherichia coli is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1016/J.PEP.2008.12.013
P8608Fatcat IDrelease_jrlxgsztbjeh3cc7doonha6ceq
P932PMC publication ID2747733
P698PubMed publication ID19162193
P5875ResearchGate publication ID23931361

P50authorSusan S WallaceQ41886664
P2093author name stringYin Guo
Viswanath Bandaru
P2860cites workDeciphering the biology of Mycobacterium tuberculosis from the complete genome sequenceQ22122411
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Pathogen DNA as target for host-generated oxidative stress: role for repair of bacterial DNA damage in Helicobacter pylori colonizationQ24554222
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Controlling gene expression in mycobacteria with anhydrotetracycline and Tet repressorQ24802513
High-level heterologous expression and secretion in rapidly growing nonpathogenic mycobacteria of four major Mycobacterium tuberculosis extracellular proteins considered to be leading vaccine candidates and drug targetsQ28487055
Secondary structure of the ribosome binding site determines translational efficiency: a quantitative analysisQ33825350
Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coliQ34058060
Codon usage in the Mycobacterium tuberculosis complexQ34063767
Localization of the target site for translational regulation of the L11 operon and direct evidence for translational coupling in Escherichia coliQ34256940
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Role of mRNA translational efficiency in bovine growth hormone expression in Escherichia coliQ37565694
Cloning and sequencing of the gene which encodes the highly inducible acetamidase of Mycobacterium smegmatisQ38321132
Enhanced production of recombinant Mycobacterium tuberculosis antigens in Escherichia coli by replacement of low-usage codonsQ39513714
Overexpression of an mRNA dependent on rare codons inhibits protein synthesis and cell growthQ39841396
Overexpression of genes of an extreme thermophile Thermus thermophilus, in Escherichia coli cellsQ39931816
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Expression of tetanus toxin fragment C in E. coli: high level expression by removing rare codonsQ40540070
The role of DNA base excision repair in the pathogenesis of Salmonella enterica serovar TyphimuriumQ40562049
Errors of heterologous protein expressionQ41238667
Screening of a mutant plasmid with high expression efficiency of GC-rich leuB gene of an extreme thermophile, Thermus thermophilus, in Escherichia coliQ42666621
Effective structure of a leader open reading frame for enhancing the expression of GC-rich genesQ42679617
Effects of codon usage versus putative 5'-mRNA structure on the expression of Fusarium solani cutinase in the Escherichia coli cytoplasmQ44268096
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Spectroscopic studies of zinc(II)- and cobalt(II)-associated Escherichia coli formamidopyrimidine-DNA glycosylase: extended X-ray absorption fine structure evidence for a metal-binding domain.Q50509102
The RD1 proteins of Mycobacterium tuberculosis: expression in Mycobacterium smegmatis and biochemical characterization.Q52948905
Consecutive low-usage leucine codons block translation only when near the 5' end of a message in Escherichia coli.Q54617430
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Translational coupling at an intercistronic boundary of the Escherichia coli galactose operonQ62029029
Oxidative DNA Glycosylases: Recipes from Cloning to CharacterizationQ62061093
Overexpression of the Thermus aquaticus B malate dehydrogenase-encoding gene in Escherichia coliQ68127392
Codon usage determines translation rate in Escherichia coliQ69088273
A high-level prokaryotic expression system: synthesis of human interleukin 1 alpha and its receptor antagonistQ71818070
Secondary structure of mRNA and efficiency of translation initiationQ72136171
NH2-terminal proline acts as a nucleophile in the glycosylase/AP-lyase reaction catalyzed by Escherichia coli formamidopyrimidine-DNA glycosylase (Fpg) proteinQ73067687
Gene expression, amino acid conservation, and hydrophobicity are the main factors shaping codon preferences in Mycobacterium tuberculosis and Mycobacterium lepraeQ73400095
An inducible expression system permitting the efficient purification of a recombinant antigen from Mycobacterium smegmatisQ77534586
Chemistry of Glycosylases and Endonucleases Involved in Base-Excision RepairQ77646696
P433issue2
P921main subjectEscherichia coliQ25419
Mycobacterium tuberculosisQ130971
overexpressionQ61643320
P304page(s)230-237
P577publication date2008-12-30
P1433published inProtein Expression and PurificationQ7251430
P1476titleA novel bicistronic vector for overexpressing Mycobacterium tuberculosis proteins in Escherichia coli
P478volume65