Adenovirus vector-based incorporation of a photo-cross-linkable amino acid into proteins in human primary cells and cancerous cell lines

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Adenovirus vector-based incorporation of a photo-cross-linkable amino acid into proteins in human primary cells and cancerous cell lines is …
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scholarly articleQ13442814

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P356DOI10.1038/SREP36946
P932PMC publication ID5105139
P698PubMed publication ID27833131

P50authorHiroyuki MizuguchiQ64398820
P2093author name stringSeiji Takashima
Kenji Ishimoto
Takefumi Doi
Kensaku Sakamoto
Nobumasa Hino
Jun Adachi
Takeshi Tomonaga
Yoshiaki Okada
Kazuaki Takafuji
Ryohei Narumi
Ayami Kita
Kohji Hirota
Sakiko Higashi
P2860cites workWide-range protein photo-crosslinking achieved by a genetically encoded N(ε)-(benzyloxycarbonyl)lysine derivative with a diazirinyl moiety.Q51761316
Photo-lysine captures proteins that bind lysine post-translational modifications.Q52986452
MDA-468, a human breast cancer cell line with a high number of epidermal growth factor (EGF) receptors, has an amplified EGF receptor gene and is growth inhibited by EGF.Q54451512
Site-specific incorporation of non-natural amino acids into proteins in mammalian cells with an expanded genetic codeQ57894888
A simple method for constructing E1- and E1/E4-deleted recombinant adenoviral vectorsQ64381304
The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12Q64383973
Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells.Q24540391
Multistep engineering of pyrrolysyl-tRNA synthetase to genetically encode N(epsilon)-(o-azidobenzyloxycarbonyl) lysine for site-specific protein modificationQ27652963
NEMO trimerizes through its coiled-coil C-terminal domainQ28205098
Photo-leucine and photo-methionine allow identification of protein-protein interactions in living cellsQ28241088
Genetically encoded chemical probes in cells reveal the binding path of urocortin-I to CRF class B GPCRQ28572982
Adenovirus vectors for gene deliveryQ33744876
Adenovirus receptorsQ33984229
Approaches for generating recombinant adenovirus vectorsQ34446187
An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3.Q35748116
Co-immunoprecipitations revisited: an update on experimental concepts and their implementation for sensitive interactome investigations of endogenous proteinsQ36855210
Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells.Q37031825
Genetically encoding unnatural amino acids in neural stem cells and optically reporting voltage-sensitive domain changes in differentiated neuronsQ38968081
Genetic incorporation of a photo-crosslinkable amino acid reveals novel protein complexes with GRB2 in mammalian cellsQ39615825
A protocol for rapid generation of recombinant adenoviruses using the AdEasy systemQ40124490
Efficient construction of a recombinant adenovirus vector by an improved in vitro ligation methodQ40988235
Adenoviral vectors for gene transferQ41628950
Mapping the ligand-binding site on a G protein-coupled receptor (GPCR) using genetically encoded photocrosslinkersQ42737253
A versatile platform for single- and multiple-unnatural amino acid mutagenesis in Escherichia coliQ42836961
Circumventing recombination events encountered with production of a clinical-grade adenoviral vector with a double-expression cassetteQ45861650
Protein photo-cross-linking in mammalian cells by site-specific incorporation of a photoreactive amino acidQ46396181
Efficient gene transfer into mouse embryonic stem cells with adenovirus vectorsQ46541016
Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetasesQ46601418
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P4510describes a project that usesImageQuantQ112270642
P407language of work or nameEnglishQ1860
P921main subjectAdenoviridaeQ193447
P304page(s)36946
P577publication date2016-11-11
P1433published inScientific ReportsQ2261792
P1476titleAdenovirus vector-based incorporation of a photo-cross-linkable amino acid into proteins in human primary cells and cancerous cell lines
P478volume6