Minimizing the unpredictability of transgene expression in plants: the role of genetic insulators

scientific article published on 11 October 2011

Minimizing the unpredictability of transgene expression in plants: the role of genetic insulators is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1007/S00299-011-1167-Y
P698PubMed publication ID21987122

P2093author name stringZongrang Liu
Stacy D Singer
Kerik D Cox
P2860cites workNovel genes influencing the expression of the yellow locus and mdg4 (gypsy) in Drosophila melanogaster.Q52453551
A nuclear matrix/scaffold attachment region co-localizes with the gypsy retrotransposon insulator sequence.Q52561330
Upstream element of the sea urchin arylsulfatase gene serves as an insulator.Q52573489
The Idefix enhancer-blocking insulator also harbors barrier activity.Q52699380
TELOMERASE ACTIVATOR1 induces telomerase activity and potentiates responses to auxin in Arabidopsis.Q53882699
The 35S promoter used in a selectable marker gene of a plant transformation vector affects the expression of the transgeneQ57167518
Matrix attachment regions increase transgene expression levels and stability in transgenic rice plants and their progenyQ57523285
A nuclear DNA attachment element mediates elevated and position-independent gene activityQ59073618
Stable high-level transgene expression inArabidopsis thalianausing gene silencing mutants and matrix attachment regionsQ60544117
Insulation from viral transcriptional regulatory elements enables improvement to hepatoma-specific gene expression from adenovirus vectorsQ64377519
An SAR sequence containing 395 bp DNA fragment mediates enhanced, gene-dosage-correlated expression of a chimaeric heat shock gene in transgenic tobacco plantsQ70765956
Functional dissection of the promoter of the pollen-specific gene NTP303 reveals a novel pollen-specific, and conserved cis-regulatory elementQ72011212
Molecular analysis of transgenic plants generated by microprojectile bombardment: effect of petunia transformation booster sequenceQ72435996
Some like it sticky: targeting of the rice gene WaxyQ73028985
A DNA-binding element for a steroid receptor-binding factor is flanked by dual nuclear matrix DNA attachment sites in the c-myc gene promoterQ73718016
Reduced Position Effect in Mature Transgenic Plants Conferred by the Chicken Lysozyme Matrix-Associated RegionQ74817429
Strategies to mitigate transgene-promoter interactionsQ83978436
Analysis of the enhancer-blocking function of the TBS element from Petunia hybrida in transgenic Arabidopsis thaliana and Nicotiana tabacumQ84449238
An exceptionally conserved transcriptional repressor, CTCF, employs different combinations of zinc fingers to bind diverged promoter sequences of avian and mammalian c-myc oncogenesQ24316326
RNA polymerase III and RNA polymerase II promoter complexes are heterochromatin barriers in Saccharomyces cerevisiaeQ24536343
Analysis of the vertebrate insulator protein CTCF-binding sites in the human genomeQ24648552
Two modes of transvection: enhancer action in trans and bypass of a chromatin insulator in cisQ24655579
Conservation, diversification and expansion of C2H2 zinc finger proteins in the Arabidopsis thaliana genomeQ24793492
SAGE is far more sensitive than EST for detecting low-abundance transcriptsQ24804831
A bivalent chromatin structure marks key developmental genes in embryonic stem cellsQ27860977
The protein CTCF is required for the enhancer blocking activity of vertebrate insulatorsQ28142800
Methylation of a CTCF-dependent boundary controls imprinted expression of the Igf2 geneQ28145754
CTCF mediates methylation-sensitive enhancer-blocking activity at the H19/Igf2 locusQ28145756
CTCF tethers an insulator to subnuclear sites, suggesting shared insulator mechanisms across speciesQ28242132
Insulators: exploiting transcriptional and epigenetic mechanismsQ28257767
Functional noncoding sequences derived from SINEs in the mammalian genomeQ28768519
A novel CpG-free vertebrate insulator silences the testis-specific SP-10 gene in somatic tissues: role for TDP-43 in insulator functionQ30444718
VEZF1 elements mediate protection from DNA methylationQ33523011
Position effects and epigenetic silencing of plant transgenesQ33538759
Distant liaisons: long-range enhancer-promoter interactions in Drosophila.Q33745064
On the molecular mechanism of gypsy-induced mutations at the yellow locus of Drosophila melanogasterQ33880510
Transcriptional augmentation: modulation of gene expression by scaffold/matrix-attached regions (S/MAR elements).Q33920302
Human matrix attachment regions insulate transgene expression from chromosomal position effects in Drosophila melanogasterQ33996776
Use of matrix attachment regions (MARs) to minimize transgene silencing.Q34041282
Position-effect protection and enhancer blocking by the chicken beta-globin insulator are separable activitiesQ34067751
The gypsy insulator of Drosophila melanogaster, together with its binding protein suppressor of Hairy-wing, facilitate high and precise expression of transgenes in Arabidopsis thalianaQ34070754
CTCF is conserved from Drosophila to humans and confers enhancer blocking of the Fab-8 insulatorQ34166628
Molecular farming in plants: host systems and expression technologyQ34277628
Visualization of chromosomal domains with boundary element-associated factor BEAF-32.Q34313007
A 5' element of the chicken beta-globin domain serves as an insulator in human erythroid cells and protects against position effect in DrosophilaQ34352447
Identification of Arabidopsis thaliana transformants without selection reveals a high occurrence of silenced T-DNA integrationsQ34385811
Characterization of the intergenic RNA profile at abdominal-A and Abdominal-B in the Drosophila bithorax complexQ34430162
Transcription defines the embryonic domains of cis-regulatory activity at the Drosophila bithorax complexQ34430171
Insulators: many functions, many mechanisms.Q34515901
Euchromatin and pericentromeric heterochromatin: comparative composition in the tomato genomeQ34588647
Dioxin receptor and SLUG transcription factors regulate the insulator activity of B1 SINE retrotransposons via an RNA polymerase switch.Q34605617
A position-effect assay for boundaries of higher order chromosomal domainsQ34778762
A barrier-only boundary element delimits the formation of facultative heterochromatin in Drosophila melanogaster and vertebratesQ35096621
A barrier-type insulator forms a boundary between active and inactive chromatin at the murine TCRβ locusQ35163141
Engineering crops, a deserving venture.Q35173767
UASrpg can function as a heterochromatin boundary element in yeastQ35203846
The Zw5 protein, a component of the scs chromatin domain boundary, is able to block enhancer-promoter interactionQ35203899
Loss of transcriptional activity of a transgene is accompanied by DNA methylation and histone deacetylation and is prevented by insulatorsQ35209588
Characterization of the chicken beta-globin insulatorQ35956059
Protein:protein interactions and the pairing of boundary elements in vivoQ35964277
The matrix attachment regions of the chicken lysozyme gene co-map with the boundaries of the chromatin domain.Q35978428
A facilitated tracking and transcription mechanism of long-range enhancer functionQ36059486
USF1 recruits histone modification complexes and is critical for maintenance of a chromatin barrierQ36315893
Insulators and promoters: closer than we thinkQ36337521
We gather together: insulators and genome organizationQ36407771
The matrix attachment region-binding protein SATB1 participates in negative regulation of tissue-specific gene expressionQ36571699
'Insulator bodies' are aggregates of proteins but not of insulatorsQ36638827
An enhancer-blocking element between alpha and delta gene segments within the human T cell receptor alpha/delta locusQ36769509
The ins and outs of gene regulation and chromosome territory organisationQ36805999
A group of scs elements function as domain boundaries in an enhancer-blocking assayQ36815755
Boundaries. Boundaries...Boundaries???Q37182068
Chromatin loops in gene regulationQ37233805
Hydrodynamic studies on defined heterochromatin fragments support a 30-nm fiber having six nucleosomes per turnQ37413887
Effects of the su(Hw) insulator protein on the expression of the divergently transcribed Drosophila yolk protein genesQ37626611
Enhancer-promoter interference and its prevention in transgenic plantsQ37822314
Heritable transgene expression pattern imposed onto maize ubiquitin promoter by maize adh-1 matrix attachment regions: tissue and developmental specificity in maize transgenic plantsQ38341620
High-level transgene expression in plant cells: effects of a strong scaffold attachment region from tobacco.Q38358283
A transformation booster sequence (TBS) from Petunia hybrida functions as an enhancer-blocking insulator in Arabidopsis thaliana.Q38473534
Isolation of pea matrix attachment region and study on its function in transgenic tobaccosQ38475863
Matrix attachment region from the chicken lysozyme locus reduces variability in transgene expression and confers copy number-dependence in transgenic rice plantsQ38489348
TM2, a novel strong matrix attachment region isolated from tobacco, increases transgene expression in transgenic rice calli and plants.Q38489503
The rb7 matrix attachment region increases the likelihood and magnitude of transgene expression in tobacco cells: a flow cytometric studyQ38489512
Stable transformation of Theobroma cacao L. and influence of matrix attachment regions on GFP expressionQ38493626
Elevation of transgene expression level by flanking matrix attachment regions (MAR) is promoter dependent: a study of the interactions of six promoters with the RB7 3' MAR.Q38494086
Suppression of transgene silencing by matrix attachment regions in maize: a dual role for the maize 5' ADH1 matrix attachment regionQ38495361
Matrix attachment regions (MARs) enhance transformation frequencies and reduce variance of transgene expression in barleyQ38495963
The beta-phaseolin 5' matrix attachment region acts as an enhancer facilitatorQ38502867
The heat shock cognate 80 gene of tomato is flanked by matrix attachment regionsQ38503723
Dissection of the ability of the chicken lysozyme gene 5' matrix attachment region to stimulate transgene expression and to dampen position effectsQ38503958
Isolation of matrices from maize leaf nuclei: identification of a matrix-binding site adjacent to the Adh1 geneQ38507546
The chicken lysozyme 5' matrix attachment region increases transcription from a heterologous promoter in heterologous cells and dampens position effects on the expression of transfected genesQ38509923
Gene regulation by proteins acting nearby and at a distanceQ39504312
The sea urchin sns5 insulator protects retroviral vectors from chromosomal position effects by maintaining active chromatin structure.Q39509837
Insulation from viral transcriptional regulatory elements improves inducible transgene expression from adenovirus vectors in vitro and in vivoQ40878847
The Drosophila su(Hw) gene, which controls the phenotypic effect of the gypsy transposable element, encodes a putative DNA-binding proteinQ41254885
The role of insulator elements in defining domains of gene expression.Q41441712
Scaffold attachment regions increase reporter gene expression in stably transformed plant cells.Q41550925
An Alu element from the K18 gene confers position-independent expression in transgenic miceQ41712817
Nuclear location of a chromatin insulator in Drosophila melanogasterQ43154902
The cauliflower mosaic virus (CaMV) 35S promoter sequence alters the level and patterns of activity of adjacent tissue- and organ-specific gene promotersQ44475595
Isolation of transcriptionally regulated root-specific genes from tobaccoQ44866599
Expression of the cut locus in the Drosophila wing margin is required for cell type specification and is regulated by a distant enhancerQ44897934
Properties of an isolated transcription stimulating sequence derived from the cauliflower mosaic virus 35S promoterQ45348777
Both the constitutive cauliflower mosaic virus 35S and tissue-specific AGAMOUS enhancers activate transcription autonomously in Arabidopsis thalianaQ45373796
Origins of extrinsic variability in eukaryotic gene expressionQ46189027
The 5' boundary of the human apolipoprotein B chromatin domain in intestinal cellsQ46433478
The human insulin gene displays transcriptionally active epigenetic marks in islet-derived mesenchymal precursor cells in the absence of insulin expressionQ46977255
A drosophila protein that imparts directionality on a chromatin insulator is an enhancer of position-effect variegationQ47072182
Evolutionarily conserved E(y)2/Sus1 protein is essential for the barrier activity of Su(Hw)-dependent insulators in DrosophilaQ47072344
The Drosophila melanogaster suppressor of Hairy-wing protein binds to specific sequences of the gypsy retrotransposonQ47072529
A leucine zipper domain of the suppressor of Hairy-wing protein mediates its repressive effect on enhancer functionQ47072830
Matrix attachment regions and transcribed sequences within a long chromosomal continuum containing maize Adh1.Q48070247
An insulator element from the sea urchin Hemicentrotus pulcherrimus suppresses variation in transgene expression in cultured tobacco cellsQ48359914
Characterization of a plant scaffold attachment region in a DNA fragment that normalizes transgene expression in tobaccoQ49169185
Creation and analysis of a novel chimeric promoter for the complete containment of pollen- and seed-mediated gene flow.Q51712158
The Fab-8 boundary defines the distal limit of the bithorax complex iab-7 domain and insulates iab-7 from initiation elements and a PRE in the adjacent iab-8 domain.Q52171418
P433issue1
P304page(s)13-25
P577publication date2011-10-11
P1433published inPlant Cell ReportsQ7201465
P1476titleMinimizing the unpredictability of transgene expression in plants: the role of genetic insulators
P478volume31

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cites work (P2860)
Q47593191A Brief History of Promoter Development for Use in Transgenic Maize Applications
Q38454834A chimeric cry8Ea1 gene flanked by MARs efficiently controls Holotrichia parallela
Q57471495Arabidopsis UMAMIT24 and 25 are amino acid exporters involved in seed loading
Q36885589Genetic Screening for Arabidopsis Mutants Defective in STA1 Regulation under Thermal Stress Implicates the Existence of Regulators of Its Specific Expression, and the Genetic Interactions in the Stress Signaling Pathways
Q28552635Multiple Patterns of Regulation and Overexpression of a Ribonuclease-Like Pathogenesis-Related Protein Gene, OsPR10a, Conferring Disease Resistance in Rice and Arabidopsis
Q44213043Screening promoters for Anthurium transformation using transient expression
Q64087079The Rapid Methylation of T-DNAs Upon Inoculation in Plant Leaves
Q42328464Towards the development of a sustainable soya bean-based feedstock for aquaculture

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