Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris

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Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris is …
instance of (P31):
scholarly articleQ13442814

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P6179Dimensions Publication ID1051570494
P356DOI10.1186/S12934-016-0432-8
P932PMC publication ID4744420
P698PubMed publication ID26849882
P5875ResearchGate publication ID293190063

P50authorRochelle AwQ59696469
Karen M PolizziQ46305395
P2093author name stringKaren M Polizzi
Rochelle Aw
P2860cites workMultiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastorisQ46441118
Posttransformational vector amplification in the yeast Pichia pastorisQ47445210
Efficient generation of multi-copy strains for optimizing secretory expression of porcine insulin precursor in yeast Pichia pastorisQ47680232
The generation of multicopy recombinant strainsQ47780725
The effect of gene copy number and co-expression of chaperone on production of albumin fusion proteins in Pichia pastorisQ48047224
Enhancement of protein secretion in Pichia pastoris by overexpression of protein disulfide isomerase.Q51329232
High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the geneQ67527996
Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copiesQ68038239
Can too many copies spoil the broth?Q26865796
Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene expression and synthetic biologyQ28727694
Systematic single-cell analysis of Pichia pastoris reveals secretory capacity limits productivityQ28728709
Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastorisQ33898335
G-418, an elongation inhibitor of 80 S ribosomesQ34707729
A new selective agent for eukaryotic cloning vectorsQ34714375
Protein folding and quality control in the endoplasmic reticulum: Recent lessons from yeast and mammalian cell systemsQ35160159
Stress in recombinant protein producing yeasts.Q35893718
Heterologous protein production using the Pichia pastoris expression system.Q36036987
Gene amplification, radiation sensitivity and DNA double-strand breaksQ37679403
Evaluating the influence of selection markers on obtaining selected pools and stable cell lines in human cellsQ39186977
The effects of G418 on the growth and metabolism of recombinant mammalian cell linesQ39918128
Cassettes of theStreptoalloteichus hindustanus blegene for transformation of lower and higher eukaryotes to phleomycin resistanceQ40518218
Pichia pastoris as a host system for transformationsQ40676523
Direct selection of Pichia pastoris expression strains using new G418 resistance vectorsQ41715811
In Pichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response.Q41808596
Directed gene copy number amplification in Pichia pastoris by vector integration into the ribosomal DNA locus.Q43624481
Cyclic fed-batch culture for production of human serum albumin in Pichia pastorisQ44389334
Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastorisQ44750733
P921main subjectPichia pastorisQ148697
P304page(s)29
P577publication date2016-02-05
P1433published inMicrobial Cell FactoriesQ15766995
P1476titleLiquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris
P478volume15

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cites work (P2860)
Q42094194A Mitochondrial Autonomously Replicating Sequence from Pichia pastoris for Uniform High Level Recombinant Protein Production
Q92755065CRISPR-Cas9-mediated genomic multiloci integration in Pichia pastoris
Q48252015Effect of plasmid design and type of integration event on recombinant protein expression in Pichia pastoris
Q37721762Expressing anti-HIV VRC01 antibody using the murine IgG1 secretion signal in Pichia pastoris.
Q50221377Molecular strategies to increase the levels of heterologous transcripts in Komagataella phaffii for protein production
Q42211412Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker
Q38851411Production of Chimeric Acidic α-Amylase by the Recombinant Pichia pastoris and Its Applications

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