Next-generation sequencing can reveal in vitro-generated PCR crossover products: some artifactual sequences correspond to HLA alleles in the IMGT/HLA database

scientific article published on January 2014

Next-generation sequencing can reveal in vitro-generated PCR crossover products: some artifactual sequences correspond to HLA alleles in the IMGT/HLA database is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1111/TAN.12269
P698PubMed publication ID24355006
P5875ResearchGate publication ID259393043

P50authorHenry A. ErlichQ110157647
P2093author name stringD Goodridge
A M Lazaro
T C Williams
M Tilanus
C L Holcomb
M Rastrou
P2860cites workPrimer-directed enzymatic amplification of DNA with a thermostable DNA polymeraseQ26778389
Polymorphism of human Ia antigens: gene conversion between two DR beta loci results in a new HLA-D/DR specificityQ28298610
Structural comparison of the genes of two HLA-DR supertypic groups: the loci encoding DRw52 and DRw53 are not truly allelicQ28301301
Universal amplification, next-generation sequencing, and assembly of HIV-1 genomesQ30421453
At least 1 in 20 16S rRNA sequence records currently held in public repositories is estimated to contain substantial anomaliesQ33228706
Sample richness and genetic diversity as drivers of chimera formation in nSSU metagenetic analysesQ34141458
High-resolution, high-throughput HLA genotyping by next-generation sequencingQ34380961
High throughput HLA genotyping using 454 sequencing and the Fluidigm Access Array™ System for simplified amplicon library preparation.Q34582241
Rapid, scalable and highly automated HLA genotyping using next-generation sequencing: a transition from research to diagnosticsQ34655295
Artificial recombination may influence the evolutionary analysis of Newcastle disease virusQ35383109
Analysis of 454 sequencing error rate, error sources, and artifact recombination for detection of Low-frequency drug resistance mutations in HIV-1 DNA.Q36690550
HLA DNA typing: past, present, and futureQ38014961
A multi-site study using high-resolution HLA genotyping by next generation sequencingQ38494977
Hybrid DNA artifact from PCR of closely related target sequences.Q40450365
DNA recombination during PCR.Q40515497
Identification of a novel HLA-DRB3*01 variant, HLA-DRB3*0114, containing a DRB1 sequence motif by micro-temperature gradient gel electrophoresis confirmed by sequence-based typingQ45765588
The IMGT/HLA databaseQ56879486
P433issue1
P921main subjectdatabaseQ8513
P304page(s)32-40
P577publication date2014-01-01
P1433published inTissue AntigensQ1573131
P1476titleNext-generation sequencing can reveal in vitro-generated PCR crossover products: some artifactual sequences correspond to HLA alleles in the IMGT/HLA database
P478volume83

Reverse relations

cites work (P2860)
Q39074755454 screening of individual MHC variation in an endemic island passerine
Q40632388Characterization of MHC class II B polymorphism in multiple populations of wild gorillas using non-invasive samples and next-generation sequencing
Q41905932Cost-efficient high-throughput HLA typing by MiSeq amplicon sequencing
Q35504310Cost-efficient multiplex PCR for routine genotyping of up to nine classical HLA loci in a single analytical run of multiple samples by next generation sequencing.
Q35647298Development and validation of a sample sparing strategy for HLA typing utilizing next generation sequencing
Q35549634Effect of the enzyme and PCR conditions on the quality of high-throughput DNA sequencing results
Q36242890Examining Sources of Error in PCR by Single-Molecule Sequencing.
Q31039365HAPCAD: An open-source tool to detect PCR crossovers in next-generation sequencing generated HLA data
Q38180493HLA typing by next-generation sequencing - getting closer to reality.
Q38983113High-throughput next-generation sequencing to genotype six classical HLA loci from 96 donors in a single MiSeq run.
Q35850731Immune Repertoire Profiling Reveals that Clonally Expanded B and T Cells Infiltrating Diseased Human Kidneys Can Also Be Tracked in Blood
Q90460567Long-Term Outcome and Rejection After Allogeneic Uterus Transplantation in Cynomolgus Macaques
Q38449812Next-generation HLA sequencing using the 454 GS FLX system
Q35526762Power laws for heavy-tailed distributions: modeling allele and haplotype diversity for the national marrow donor program
Q58583841Reference Grade Characterization of Polymorphisms in Full-Length HLA Class I and II Genes With Short-Read Sequencing on the ION PGM System and Long-Reads Generated by Single Molecule, Real-Time Sequencing on the PacBio Platform